Direct imaging of capillaries reveals the mechanism of arteriovenous interlacing in the chick chorioallantoic membrane

Richard, Sophie; Brun, Amanda; Tedesco, Antônio Cláudio; Gallois, Benjamin; Taghi, Naoual; Dantan, Philippe; Séguin, Johanne

doi:10.1038/s42003-018-0229-x

Cited by 6 publications

(4 citation statements)

References 35 publications

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“…The maturity of blood vessels may be contributed to these variations, also the process of angiogenesis requires more quantity and quality of blood vessels in the embryo [22,23]. Our results reveal that the effects of PGF 2 α analogue on the blood vessels may be influenced in a time-dependent manner, so many interacting factors play a key role in VEGF gene expression [24].…”

Section: Molecular Study a Mrna Isolation: Mrna Isolated From Cammentioning

confidence: 68%

VEGF Gene Expression and Angiogenesis in the Chorioallantoic Membrane: the Role of Cloprostenol

Abdullah

shindala

Mustafa

2023

Egyptian Journal of Veterinary Sciences

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B ACKGROUND: Cloprostenol, as a common clinically used PGF 2 α analogue, is widely employed in veterinary practice to induce parturition. It is main action, as a potent luteolytic, carried by vasoconstriction of the blood vessels that supply the corpus luteum. While vascular endothelial growth factor (VEGF) is a key regulator of angiogenesis. Objective: This study aimed to investigate the precise effects of PGF 2 α analogue (cloprostenol) on the VEGF gene expression. Methods: Chorioallantoic membranes (CAM) of local chicken fertilized eggs were used at 6 and 9 days of incubation. The control group, without treatment, and other groups treated with (22.5µg/egg) of cloprostenol sodium (Galapan) ® and eggs were re-incubated. CAM blood vessels were observed and documented and samples of them were isolated at 1, 2, 4, 6, 12, and 24 hours after incubation. mRNA was isolated and converted to cDNA, and RT-PCR was done to determine the gene expression of VEGF. Results: It shows that the peak of gene expression of VEGF gene was at 24 hours of day 9 of incubation. Furthermore, the best antiangiogenic effects were at the same time of 6 hours of day 6 of incubation. Conclusion: It could be concluded that PGF 2 α analogue (cloprostenol) has vasoactive properties depend particularly on the VEGF, this action is in a time-dependent manner and may be based on the hypoxia-induced pathways that elicited by vasoconstrictive action of cloprostenol.

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Section: Molecular Study a Mrna Isolation: Mrna Isolated From Cammentioning

confidence: 68%

VEGF Gene Expression and Angiogenesis in the Chorioallantoic Membrane: the Role of Cloprostenol

Abdullah

shindala

Mustafa

2023

Egyptian Journal of Veterinary Sciences

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“…We have developed a custom macro to register the frames prior to extracting the blood vessels by the Z-stack (min) function in ImageJ. This produces a projection of the darkest erythrocytes, and the result is the formation of a composite image forming a digital cast of the blood vessels, in vivo, without any staining 38 . Imaging of the sagittal vessels in vivo is particularly challenging.…”

Section: Methodsmentioning

confidence: 99%

“…Then, a movie of the blood flow is generated and integrated following the procedure described in Ref. 38 , in order to get the enhanced images of Fig. 6 D. Images were acquired at different orientations for the HH21 stage (Fig.…”

Section: Methodsmentioning

confidence: 99%

Electrical stimulation of chicken embryo development supports the Inside story scenario of human development and evolution

Fleury

2024

Sci Rep

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Animal evolution is driven by random mutations at the genome level. However, it has long been suggested that there exist physical constraints which limit the set of possible outcomes. In craniate evolution, it has been observed that head features, notably in the genus homo, can be ordered in a morphological diagram such that, as the brain expands, the head rocks more forward, face features become less prognathous and the mouth tends to recede. One school of paleontologists suggests that this trend is wired somewhere structurally inside the anatomy, and that random modifications of genes push up or down animal forms along a pre-determined path. No actual experiment has been able to settle the dispute. I present here an experiment of electric stimulation of the head in the chicken embryo which is able to enhance the magnitude of tension forces during development. This experimental intervention causes a correlated brain shrinkage and rotatory movement of the head, congruent with tissue texture, which proves that head dilation and flexure are intimately linked. Numerical modelling explains why the brain curls when it dilates. This gives support to the idea that there exists, in the texture of the vertebrate embryo, a latent dynamic pattern for the observed paleontological trends in craniates towards homo, a concept known as Inside story.

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“…The chick chorioallantoic membrane (CAM) presents a versatile in vivo model for the study of angiogenesis and vascular adaptation [ 30 ] as it facilitates optical access to a developing vascular system in a transparent, two-dimensional layout. Importantly, the CAM allows for extended observation periods for up to 1–2 days, permitting to follow the successive steps of angiogenic development over time [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Coalescent angiogenesis—evidence for a novel concept of vascular network maturation

et al. 2021

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Angiogenesis describes the formation of new blood vessels from pre-existing vascular structures. While the most studied mode of angiogenesis is vascular sprouting, specific conditions or organs favor intussusception, i.e., the division or splitting of an existing vessel, as preferential mode of new vessel formation. In the present study, sustained (33-h) intravital microscopy of the vasculature in the chick chorioallantoic membrane (CAM) led to the hypothesis of a novel non-sprouting mode for vessel generation, which we termed “coalescent angiogenesis.” In this process, preferential flow pathways evolve from isotropic capillary meshes enclosing tissue islands. These preferential flow pathways progressively enlarge by coalescence of capillaries and elimination of internal tissue pillars, in a process that is the reverse of intussusception. Concomitantly, less perfused segments regress. In this way, an initially mesh-like capillary network is remodeled into a tree structure, while conserving vascular wall components and maintaining blood flow. Coalescent angiogenesis, thus, describes the remodeling of an initial, hemodynamically inefficient mesh structure, into a hierarchical tree structure that provides efficient convective transport, allowing for the rapid expansion of the vasculature with maintained blood supply and function during development.

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Direct imaging of capillaries reveals the mechanism of arteriovenous interlacing in the chick chorioallantoic membrane

Cited by 6 publications

References 35 publications

VEGF Gene Expression and Angiogenesis in the Chorioallantoic Membrane: the Role of Cloprostenol

VEGF Gene Expression and Angiogenesis in the Chorioallantoic Membrane: the Role of Cloprostenol

Electrical stimulation of chicken embryo development supports the Inside story scenario of human development and evolution

Coalescent angiogenesis—evidence for a novel concept of vascular network maturation

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