Direct immune-detection of cortisol by chemiresistor graphene oxide sensor

Kim, Yo-Han; Lee, Kyung-Min; Jung, Hunsang; Kang, Hee Kyung; Jo, Jihoon; Park, In Kyu; Lee, Hyun Ho

doi:10.1016/j.bios.2017.07.017

Cited by 70 publications

(32 citation statements)

References 19 publications

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“…In these studies, the detection of the cortisol is achieved by the inhibition of the surface for redox reaction [19][20][21][22][23] . In addition, chemiresistor-immunosensors followed a similar strategy 24,25 . Among the electrochemical sensors, the ones using graphene and its derivatives 26…”

mentioning

confidence: 99%

Extended gate field-effect-transistor for sensing cortisol stress hormone

et al. 2021

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Cortisol is a hormone released in response to stress and is a major glucocorticoid produced by adrenal glands. Here, we report a wearable sensory electronic chip using label-free detection, based on a platinum/graphene aptamer extended gate field effect transistor (EG-FET) for the recognition of cortisol in biological buffers within the Debye screening length. The device shows promising experimental features for real-time monitoring of the circadian rhythm of cortisol in human sweat. We report a hysteresis-free EG-FET with a voltage sensitivity of the order of 14 mV/decade and current sensitivity up to 80% over the four decades of cortisol concentration. The detection limit is 0.2 nM over a wide range, between 1 nM and 10 µM, of cortisol concentrations in physiological fluid, with negligible drift over time and high selectivity. The dynamic range fully covers those in human sweat. We propose a comprehensive analysis and a unified, predictive analytical mapping of current sensitivity in all regimes of operation.

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confidence: 99%

Extended gate field-effect-transistor for sensing cortisol stress hormone

et al. 2021

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“…Molecularly imprinted polymers (MIPs) are recently studied as alternatives and used in assays to overcome the economic downsides of antibody-based assays [ 43 ]. Similarly, bio- or chemiluminescent assays may serve as alternatives [ [44] , [45] , [46] , [47] , [48] , [49] ].…”

Section: Assaysmentioning

confidence: 99%

“…In addition to classical immunoassays, immunological methods and electrochemical analysis are often combined in stress marker analyses [ [50] , [51] , [52] , [53] , [54] , [55] ]. Bio- and immunosensors are widespread thanks to their versatility, easy use, sensitivity, and short analysis time [ 45 , 46 , [50] , [51] , [52] , [53] , [54] , [55] , [56] ]. Furthermore, due to their possibility of miniaturization, they offer an excellent opportunity for developing point of care (POC) devices.…”

Section: Assaysmentioning

confidence: 99%

“… Saliva collection LOD Comment Bioluminescence [ 44 ] n.s. 360 pg/mL Chemiresistor Immunosensor [ 45 ] [ 46 ] Artificial saliva n.s. 1 pg/mL 10 pg/mL Single-walled, carbon nanotube-based chemiresistive transducer c-Mab covalently immobilized on rGO CL [ 47 ] Artificial saliva 80 pg/mL CLIA [ 110 ] [ 111 ] Sponge Artificial saliva 160 pg/mL 0.47 pg/mL In pigs, SPE Electrochemical biosensor (immunosensor and IA) [ 50 ] [ 51 ] [ 52 ] [ 53 ] [ 54 ] [ 55 ] Salivette® PBS solution Artificial saliva n.s.…”

Section: Stress Related Biomarkersmentioning

confidence: 99%

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Current methods for stress marker detection in saliva

Giacomello

Scholten

Parr

2020

Journal of Pharmaceutical and Biomedical Analysis

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“…Recently, electrochemical sensors [25][26][27], surface plasmon resonance (SPR) sensors [28] and impedimetric biosensors [29] have been introduced into cortisol investigation and achieved great progress, even though these technologies require mediators, complex electrode fabrication, indirect cortisol measurement, high-voltage investigation, or modification of the analyte itself. Generally, extensive efforts regarding high-sensitivity cortisol estimation via electrochemical immunosensors have been published and excellent detection limits of 1 pM to 1 µM have been obtained by the sensors [18,25,26,[30][31][32]. In these studies, different dimensions of nanomaterials have been utilized in the electrochemical sensors to improve the biocompatible, electroactive and biologically functional immobilization matrices.…”

Section: Introductionmentioning

confidence: 99%

Salivary Cortisol Determination on Smartphone-Based Differential Pulse Voltammetry System

Liu

Men

et al. 2020

Sensors

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Cortisol is commonly used as a significant biomarker of psychological or physical stress. With the accelerated pace of life, non-invasive cortisol detection at the point of care (POC) is in high demand for personal health monitoring. In this paper, an ultrasensitive immunosensor using gold nanoparticles/molybdenum disulfide/gold nanoparticles (AuNPs/MoS2/AuNPs) as transducer was explored for non-invasive salivary cortisol monitoring at POC with the miniaturized differential pulse voltammetry (DPV) system based on a smartphone. Covalent binding of cortisol antibody (CORT-Ab) onto the AuNPs/MoS2/AuNPs transducer was achieved through the self-assembled monolayer of specially designed polyethylene glycol (PEG, SH-PEG-COOH). Non-specific binding was avoided by passivating the surface with ethanolamine. The miniaturized portable DPV system was utilized for human salivary cortisol detection. A series current response of different cortisol concentrations decreased and exhibited a linear range of 0.5–200 nM, the detection limit of 0.11 nM, and high sensitivity of 30 μA M−1 with a regression coefficient of 0.9947. Cortisol was also distinguished successfully from the other substances in saliva. The recovery ratio of spiked human salivary cortisol and the variation of salivary cortisol level during one day indicated the practicability of the immunosensor based on the portable system. The results demonstrated the excellent performance of the smartphone-based immunosensor system and its great potential application for non-invasive human salivary cortisol detection at POC.

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Direct immune-detection of cortisol by chemiresistor graphene oxide sensor

Cited by 70 publications

References 19 publications

Extended gate field-effect-transistor for sensing cortisol stress hormone

Extended gate field-effect-transistor for sensing cortisol stress hormone

Current methods for stress marker detection in saliva

Salivary Cortisol Determination on Smartphone-Based Differential Pulse Voltammetry System

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