Direct, immunological and molecular techniques for a fasciolosis survey in a rural area of San Luis, Argentina

Abstract

Fasciolosis is a zoonosis caused by the trematode Fasciola hepatica, prevalent in cattle, that is actually emerging as a cause of disease in humans. The goal of this work was to describe the characteristics of fasciolosis in arroyo El Juncal region, La Toma, San Luis province, Argentina. In order to get this objective, a transversal, quantitative study was carried out by a fieldwork that allowed the collection of data, human, animal, and environmental samples. The materials were processed by direct, immunological and/or molecular diagnostic techniques.According to the geographical characteristics and in presence of all the definitive and intermediate hosts, reservoirs, and sources of infection, it was possible to describe the persistence of fasciolosis in the area. The prevalence was 11.90 % in humans (by serology), 5.26 % in cattle (by coprological analysis) and 61.76 % in snails (by PCR). The situation that was found for this area indicates that any measure of intervention for the control of this zoonosis should be adopted by multidisciplinary teams.

Prevalence of liver fluke infections in slaughtered animals in Lorestan, Iran

“…The trematodes F. hepatica and D. dendriticum are recognized as the most important helminthic parasites in ruminants and causing economic losses to ranchers due to reductions in milk and meat production, condemnation of parasitized livers, abortion, increased mortality and the expense of control measures (Carnevale et al 2013). In Iran, the main enzootic area of sheep liver trematodes lies in the north of Iran, but Fasciola spp.…”

Neglected Tropical Diseases - Latin America and the Caribbean

“…2009a). 2000), Colombia (see review in Bargues et al 2011c), Venezuela (see review in Bargues et al 2011b), and recently also in Argentina (Carnevale et al 2013). Human infection has also been described in altitude areas of Ecuador (Trueba et al.…”

Human fascioliasis endemic areas in Argentina: multigene characterisation of the lymnaeid vectors and climatic-environmental assessment of the transmission pattern

“…However, different epidemiological situations were recently detected in two areas of this country as a result of two serological surveys performed with an ELISA test for the detection of anti-recombinant procathepsine L1 of F. hepatica (Fh-rproCL1) antibodies [ 59 , 60 ]. In the area of Arroyo El Juncal, La Toma, province of San Luis, between 500 and 600 m altitude, the prevalence was 11.90 % in a total of 42 human subjects (2 infected out of 23 males and 3 out of 19 females) serologically analysed [ 61 ]. This corresponds to a human hyperendemic situation according to the WHO classification [ 27 ].…”

“…This corresponds to a human hyperendemic situation according to the WHO classification [ 27 ]. In the same El Juncal area, 5.26 % out of 19 livestock species proved to be infected after coprological analysis (only in cattle; sheep and horses were not infected) and a surprisingly high prevalence (61.76 % out of 34 snails) was detected by sequencing a 447 bp long fragment of the mtDNA cox 1 gene of F. hepatica [ 61 ] in snails identified as Lymnaea viator (= L. viatrix ; for nomenclature see [ 38 ]).…”