Direct infusion mass spectrometry or liquid chromatography mass spectrometry for human metabonomics? A serum metabonomic study of kidney cancer

Lin, Lin; Yu, Quan; Yan, Xiaomei; Hang, Wei; Zheng, Jiaxin; Xing, Jinchun; Huang, Benli

doi:10.1039/c0an00265h

Cited by 139 publications

(95 citation statements)

References 51 publications

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“…According to the study of Lin et al, direct-infusion MS saves time, requiring only 5% of the analysis time of LC-MS. However, LC-MS is capable of finding twice as many differential metabolites as direct-infusion MS [17].…”

Section: Methodsmentioning

confidence: 99%

Metabolomics for tumor marker discovery and identification based on chromatography–mass spectrometry

Yin

2013

Expert Review of Molecular Diagnostics

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Early detection is the most effective way to improve the clinical outcome of malignancies. Although some tumor markers are now widely used in the clinic, their sensitivity and specificity are still not satisfactory. Thus, there is an urgent requirement for the discovery of new tumor markers. By measuring holistic endogenous metabolites, metabolomics can be used for delineating metabolic networks and discovering metabolic markers. Chromatography-mass spectrometry is the most widely used tool for metabolomics and has shown great potential for biomarker screening. In this review, the authors summarize: recent advances in the protocols and methodologies of chromatography-mass spectrometry-based metabolomics in the discovery of tumor markers; recently identified tumor metabolic markers for primary liver cancer, gynecologic cancer and genitourinary cancer and their applications; and commonly encountered problems in the translational research of metabolic markers.

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Section: Methodsmentioning

confidence: 99%

Metabolomics for tumor marker discovery and identification based on chromatography–mass spectrometry

Yin

2013

Expert Review of Molecular Diagnostics

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“…DIMS provides a high-throughput system for metabolic profiling because a large number (hundreds or thousands) of samples can be analyzed in a given period of time. The use of DIMS for the large sample sets probably reflects the difficulty in maintaining reproducibility of the LC-MS technique over many weeks or months, where chromatographic drift or small changes in instrument performance can impair the data analysis (Barrett, 2012;Lin et al, 2010).…”

Section: Human Blood Metabolomementioning

confidence: 99%

Postgenomics Diagnostics: Metabolomics Approaches to Human Blood Profiling

Trifonova

Lokhov

Archakov

2013

OMICS: A Journal of Integrative Biology

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We live in exciting times with the prospects of postgenomics diagnostics. Metabolomics is a novel ''omics'' dataintensive science that is accelerating the development of postgenomics diagnostics, particularly with use of accessible peripheral tissue compartments. Metabolomics involves the study of a comprehensive set of low molecular weight substances (metabolites) present in biological systems. The metabolite profiles represent the molecular phenotype of biological systems and reflect the information encoded at the genomic level and implemented at the transcriptomic and proteomic levels. Analysis of the human blood metabolite profile is a universal and highly promising tool for clinical postgenomics applications because it reflects both the endogenous and exogenous (environmental) factors influencing an individual organism. This article presents a critical synthesis and original analysis of both the technical implementation of metabolic profiling of blood and statistical analysis of metabolite profiles for effective disease diagnostics and risk assessment in the present postgenomics era.

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“…When large-scale multi-batch experiments are designed, robust workflows have to be developed to minimize experiment analytical variation [25]. Lin et al compared the classification and biomarker discovery capacities of direct infusion ESI-MS and liquid chromatography (LC)-MS [26]. For that purpose, serum samples from kidney cancer patients and healthy controls were analyzed by both analytical techniques.…”

Section: Direct Infusion Msmentioning

confidence: 99%

Direct Mass Spectrometry-Based Approaches in Metabolomics

Ibáñez

García‐Cañas

Valdés

et al. 2014

Fundamentals of Advanced Omics Technologies: From Genes to Metabolites

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Metabolomics is one of the newest omics technologies concerned with the identification and quantification of small molecules in a high-throughput manner. Considering the number of different types of metabolites present in a wide dynamic range of concentrations in any single living system, still actual analytical technologies can only capture a part of the metabolome.Currently MS-based approaches yield a higher sensitivity than NMR when analyzing minimal amounts of complex mixtures. Most MS-based approaches in metabolomics involve a physical/chemical purification/fractionation prior to MS analysis, to avoid sample matrix effects, at the expenses of low high-throughput performance. In the quest to achieve the maximum highthroughput production of metabolite information in the largest possible number of samples an extensive array of direct ionization or desorption/ionization techniques have been developed and combined. In the present Chapter an overview of the main desorption/ionization techniques coupled to MS applied to direct metabolite profiling or fingerprinting is presented.

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Direct infusion mass spectrometry or liquid chromatography mass spectrometry for human metabonomics? A serum metabonomic study of kidney cancer

Cited by 139 publications

References 51 publications

Metabolomics for tumor marker discovery and identification based on chromatography–mass spectrometry

Metabolomics for tumor marker discovery and identification based on chromatography–mass spectrometry

Postgenomics Diagnostics: Metabolomics Approaches to Human Blood Profiling

Direct Mass Spectrometry-Based Approaches in Metabolomics

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