Direct Measurement of Glycine-Extended Adrenomedullin in Plasma and Tissue Using an Ultrasensitive Immune Complex Transfer Enzyme Immunoassay in Rats

Yamaga, Junichi; Hashida, Seiichi; Kïtamura, Kazuo; Tokashiki, Mariko; Aoki, Toshihiro; Inatsu, Haruhiko; Ishikawa, Naoto; Kangawa, Kenji; Morishita, Kazuhiro; Eto, Tanenao

doi:10.1291/hypres.26.s45

Cited by 12 publications

(4 citation statements)

References 31 publications

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“…However, it has not been possible to measure peptides, insulin, CRH, -ANP, adrenomedullin or PAMP at less than attomole levels with an ultra-sensitive assay (ICT-EIA) using two monoclonal antibodies [Hashida, 2004;Katakami, 2002;Yamaga, 2003]. The major reason for this failure appeared to be due more to a decreased signal rather than because of dilution degree.…”

Section: Discussionmentioning

confidence: 99%

Development of an Ultra-SensitiveEnzyme Immunoassay for Insulin and Its Application to the Evaluation of Diabetic Risk by Analysis of Morning Urine

Hashida¹,

Miyzawa²,

Hirajima³

et al. 2012

Trends in Immunolabelled and Related Techniques

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Section: Discussionmentioning

confidence: 99%

Development of an Ultra-SensitiveEnzyme Immunoassay for Insulin and Its Application to the Evaluation of Diabetic Risk by Analysis of Morning Urine

Hashida¹,

Miyzawa²,

Hirajima³

et al. 2012

Trends in Immunolabelled and Related Techniques

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“…ICT-EIA. b-d-Galactosidase-labeled anti-L-PGDS Fab′ and dinitrophenyl (DNP) biotin-labeled anti-L-PGDS Fab′ were prepared for ICT-EIA as previously reported (22). After L-PGDS and the two conjugates formed into an immunocomplex, they were trapped onto polystyrene beads coated with anti-DNP IgG.…”

Section: Methodsmentioning

confidence: 99%

Preparation and Application of Monoclonal Antibodies for an Immune Complex Transfer Enzyme Immunoassay of an Egg White Allergen, Lipocalin-Type Prostaglandin D Synthase

Numata

Luo

Suzuki

2023

J Nutr Sci Vitaminol

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We have previously reported that lipocalin-type prostaglandin D synthase (L-PGDS) in egg white reacts with IgE antibodies from children with egg allergies. However, antibodies against chicken L-PGDS are not commercially available, and the amount of L-PGDS in egg white is unclear. In this study, we prepared four monoclonal antibodies against chicken L-PGDS and developed a sandwich enzyme-linked immunosorbent assay (ELISA) and a highly sensitive immune complex transfer enzyme immunoassay (ICT-EIA) to quantify L-PGDS in hen egg whites. The detection sensitivity of ICT-EIA for L-PGDS (0.01 ng/mL) was 2,000-fold higher than that of ELISA, which could not be adapted to determine the amount of L-PGDS in egg white. Thus, ICT-EIA is a better method for quantification of trace allergens and expected to be applied to the quantification of other food allergens. Hen eggs (white-shelled eggs from Julia Lite hens, brown-shelled eggs, and iodine-enriched eggs from Boris Brown hens) were purchased from markets in Kochi City, Japan, and the amounts of L-PGDS in them were determined by ICT-EIA. The amounts of L-PGDS per hen egg white were: brown-shelled eggs, 1,179.36214.3 mg/egg; iodine-enriched eggs, 607.76126.1 mg/egg; and white-shelled eggs, 350.0674.1 mg/egg. These results show that the amount of L-PGDS in hen eggs varies depending on the hen lineage; it could also be affected to some extent by other factors, such as feeds and breeding environment.

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“…After reaching confluence, the cells were incubated in serum free-media with or without m-PSL (Sigma, St. Louis, Mo., USA) for 24 h. After 24 h, more than 90% of the cells were viable under these conditions. The total AM in the supernatants of the cultured rat MCs was measured using a two-site immunoenzymometric assay, as described previously [21]. …”

Section: Methodsmentioning

confidence: 99%

Increased Production of Adrenomedullin in Glomeruli from Anti-Glomerular Basement Membrane Glomerulonephritis Rats Treated with Methylprednisolone

Iwatsubo¹,

Fujimoto²,

Matsumoto³

et al. 2006

Nephron Exp Nephrol

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Background/Aims: Adrenomedullin (AM) has anti-proliferative and apoptotic effects on mesangial cells (MCs). Both effects play an important role in the progression of glomerulonephritis (GN). Glucocorticoids are widely used for the treatment of GN; however, the relationship between AM regulation in MCs or glomeruli and glucocorticoid treatment has not been clarified. Methods: Using the cultured rat MCs, AM secretion induced by methylprednisolone (m-PSL), and MC proliferation and apoptosis caused by AM were examined. In addition, the role of AM receptor antagonist, AM(22-52), was also investigated. Then, we made an anti-glomerular basement membrane (GBM) GN rat model and compared the AM expression and production in each glomeruli obtained from the control or m-PSL-treated anti-GBM GN rats. Results: In the cultured rat MCs, AM secretion was increased by m-PSL. MC proliferation was inhibited, while MC apoptosis was increased by AM. MC apoptosis was inhibited by the addition of AM(22-52). M-PSL therapy ameliorated the progression of anti-GBM GN rats. AM expression and production were increased in the glomeruli from m-PSL-treated rats compared to the controls. Conclusion: Considering the anti-proliferative and apoptotic effects of AM on MCs, increased AM in the glomeruli might participate in the improvement of glomerular lesions in anti-GBM GN rats treated with m-PSL.

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Direct Measurement of Glycine-Extended Adrenomedullin in Plasma and Tissue Using an Ultrasensitive Immune Complex Transfer Enzyme Immunoassay in Rats

Cited by 12 publications

References 31 publications

Development of an Ultra-SensitiveEnzyme Immunoassay for Insulin and Its Application to the Evaluation of Diabetic Risk by Analysis of Morning Urine

Development of an Ultra-SensitiveEnzyme Immunoassay for Insulin and Its Application to the Evaluation of Diabetic Risk by Analysis of Morning Urine

Preparation and Application of Monoclonal Antibodies for an Immune Complex Transfer Enzyme Immunoassay of an Egg White Allergen, Lipocalin-Type Prostaglandin D Synthase

Increased Production of Adrenomedullin in Glomeruli from Anti-Glomerular Basement Membrane Glomerulonephritis Rats Treated with Methylprednisolone

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