Direct measurement of hepatic indocyanine green clearance with near-infrared spectroscopy: Separate evaluation of uptake and removal

Shinohara, Hisashi; Tanaka, Akira; Kitai, Toshiyuki; Yanabu, Nobuharu; Inomoto, Takuya; Satoh, Seiji; Hatano, Etsuro; Yamaoka, Yoshio; Hirao, Konomu

doi:10.1002/hep.510230119

Cited by 117 publications

(52 citation statements)

References 27 publications

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“…The release of ICG from cells is due to cytoplasmic and biliary excretion mechanisms. Furthermore, excretion relies on the membrane potentialdependent transport system established by an ATP-utilizing pump (24).…”

Section: Discussionmentioning

confidence: 42%

“…Experimental scheme followed for the labeling with indocyanine green (ICG), imaging, and protein analysis of undifferentiated human embryonic stem cells (hESCs) and cardiomyocytes. Representative optical images of ICG-labeled hESCs in 1 ml of KSR using the optimized labeling protocol (2.0 mg ICG/ml, 60-min incubation) at different time points after labeling (1,24,48,72,96, and 120 h) showing decreasing fluorescent signal over time. The three rows represent triplicates.…”

Section: Discussionmentioning

confidence: 49%

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Labeling Human Embryonic Stem Cell-Derived Cardiomyocytes with Indocyanine Green for Noninvasive Tracking with Optical Imaging: An FDA-Compatible Alternative to Firefly Luciferase

et al. 2010

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Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) have demonstrated the ability to improve myocardial function following transplantation into an ischemic heart; however, the functional benefits are transient possibly due to poor cell retention. A diagnostic technique that could visualize transplanted hESC-CMs could help to optimize stem cell delivery techniques. Thus, the purpose of this study was to develop a labeling technique for hESCs and hESC-CMs with the FDAapproved contrast agent indocyanine green (ICG) for optical imaging (OI). hESCs were labeled with 0.5, 1.0, 2.0, and 2.5 mg/ml of ICG for 30, 45, and 60 min at 37°C. Longitudinal OI studies were performed with both hESCs and hESC-CMs. The expression of surface proteins was assessed with immunofluorescent staining. hESCs labeled with 2 mg ICG/ml for 60 min achieved maximum fluorescence. Longitudinal studies revealed that the fluorescent signal was equivalent to controls at 120 h postlabeling. The fluorescence signal of hESCs and hESC-CMs at 1, 24, and 48 h was significantly higher compared to precontrast data (p < 0.05). Immunocytochemistry revealed retention of cell-specific surface and nuclear markers postlabeling. These data demonstrate that hESCs and hESC-CMs labeled with ICG show a significant fluorescence up to 48 h and can be visualized with OI. The labeling procedure does not impair the viability or functional integrity of the cells. The technique may be useful for assessing different delivery routes in order to improve the engraftment of transplanted hESC-CMs or other stem cell progenitors.

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Section: Discussionmentioning

confidence: 42%

Section: Discussionmentioning

confidence: 49%

Labeling Human Embryonic Stem Cell-Derived Cardiomyocytes with Indocyanine Green for Noninvasive Tracking with Optical Imaging: An FDA-Compatible Alternative to Firefly Luciferase

et al. 2010

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“…are the uptake and excretion rates of fluorophores, which have physiological significance for quantitative evaluation of organ function [31]. In our previous work [7], ICG has been used to investigate the metabolic features of organs in the mouse.…”

Section: Dynamic Forward Problemsupporting

confidence: 39%

Acceleration of dynamic fluorescence molecular tomography with principal component analysis

Zhang

et al. 2015

Biomed. Opt. Express

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Dynamic fluorescence molecular tomography (FMT) is an attractive imaging technique for three-dimensionally resolving the metabolic process of fluorescent biomarkers in small animal. When combined with compartmental modeling, dynamic FMT can be used to obtain parametric images which can provide quantitative pharmacokinetic information for drug development and metabolic research. However, the computational burden of dynamic FMT is extremely huge due to its large data sets arising from the long measurement process and the densely sampling device. In this work, we propose to accelerate the reconstruction process of dynamic FMT based on principal component analysis (PCA). Taking advantage of the compression property of PCA, the dimension of the sub weight matrix used for solving the inverse problem is reduced by retaining only a few principal components which can retain most of the effective information of the sub weight matrix. Therefore, the reconstruction process of dynamic FMT can be accelerated by solving the smaller scale inverse problem. Numerical simulation and mouse experiment are performed to validate the performance of the proposed method. Results show that the proposed method can greatly accelerate the reconstruction of parametric images in dynamic FMT almost without degradation in image quality. Szabo, and Y. Wang, "CAM-CM: A signal deconvolution tool for in vivo dynamic contrast-enhanced imaging of complex tissues," Bioinformatics 27(18), 2607-2609 (2011). 34. X. Guo, X. Liu, X. Wang, F. Tian, F. Liu, B. Zhang, G. Hu, and J. Bai, "A combined fluorescence and microcomputed tomography system for small animal imaging," IEEE Trans.

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“…The use and validation of this technology in evaluating hepatic function in liver transplant recipients have been described elsewhere. 6,[8][9][10] The DDG-2001 analyzer constructs a dye densitogram (graphical representation) of serum ICG-PDR with a noninvasive optical pulse-spectrometry nasal probe. This graph of ICG-PDR is actually a decay curve, with the slope designated as k. Thus, k represents the rate of disappearance of ICG from the blood as the liver extracts it from the circulation.…”

Section: Methodsmentioning

confidence: 45%

Predicting immunosuppressant dosing in the early postoperative period with noninvasive indocyanine green elimination following orthotopic liver transplantation

et al. 2007

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Twenty adult patients undergoing orthotopic liver transplantation (OLT) were enrolled in this study, with the noninvasive indocyanine green plasma disappearance rate (ICG-PDR) measured both during and after OLT to assess the relationship between ICG-PDR and the ability of patients to achieve therapeutic postoperative tacrolimus immunosuppressant blood levels. Liver function was determined at both 2 and 18 hours post reperfusion with the ICG-PDR k value (1/min). Postoperative standard serum measures of liver function as well as liver biopsies were also collected and analyzed. The median ICG-PDR k value for the study group at 2 hours post reperfusion was 0.20 (0.16, 0.27), whereas at 18 hours post reperfusion, it was 0.22 (0.18, 0.35). The median change in the k value between the two ICG-PDR measurements was 0.05 (Ϫ0.02, 0.07) with P ϭ 0.02. There was an interaction between the postoperative day 1 (18 hours post reperfusion) ICG-PDR k value and the linear increase in the tacrolimus blood level, such that the greater the k value was, the more gradual the observed rise was in tacrolimus over time [that is, the longer it took to achieve a therapeutic blood level (Ͼ12 ng/mL), P ϭ 0.003]. Of the 16 patients that received tacrolimus, comparable dosing on a per kilogram body weight basis was observed. Also, no significant association between ICG-PDR k values and postoperative liver biopsy results was seen. This study demonstrates that the ICG-PDR measurement is a modality with the potential to assist in achieving adequate blood levels of tacrolimus following OLT. Liver Transpl 14: [46][47][48][49][50][51][52] 2008. © 2007 AASLD.Received April 20, 2007; accepted July 31, 2007. Postoperative liver allograft function can significantly influence immunosuppressant levels, and as a result, frequent dosing changes of these agents are often required. Common immunosuppressive agents including cyclosporine, tacrolimus, and sirolimus are frequently dose-adjusted, as determined by measurements of whole blood concentration. In addition, these agents are principally metabolized by hepatic cytochrome enzymes, which are variably affected by age, blood flow, ischemia reperfusion (IR) injury, and other factors.1-3 Although this approach is commonly used in clinical practice, it provides a relatively late prediction of the patient's response to these drugs and can potentially lead to a prolonged period of suboptimal levels of a given immunosuppressant in the postoperative period, posing an increased risk for rejection. The ability to assess and predict graft metabolic function could be beneficial in determining appropriate individual immunosuppressant dosing in the posttransplant period. Because of the continuing shortage of suitable cadaveric donor livers for the ever-growing number of pa-

show abstract

Direct measurement of hepatic indocyanine green clearance with near-infrared spectroscopy: Separate evaluation of uptake and removal

Cited by 117 publications

References 27 publications

Labeling Human Embryonic Stem Cell-Derived Cardiomyocytes with Indocyanine Green for Noninvasive Tracking with Optical Imaging: An FDA-Compatible Alternative to Firefly Luciferase

Labeling Human Embryonic Stem Cell-Derived Cardiomyocytes with Indocyanine Green for Noninvasive Tracking with Optical Imaging: An FDA-Compatible Alternative to Firefly Luciferase

Acceleration of dynamic fluorescence molecular tomography with principal component analysis

Predicting immunosuppressant dosing in the early postoperative period with noninvasive indocyanine green elimination following orthotopic liver transplantation

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