Direct Measurement of Human Lung Cancerous and Noncancerous Tissues by Fourier Transform Infrared Microscopy: Can an Infrared Microscope Be Used as a Clinical Tool?

Yano, Kazuyuki; Ohoshima, Susumu; Gotou, Y.; Kumaido, Kuniyoshi; Moriguchi, Takeshi; Katayama, Hiroo

doi:10.1006/abio.2000.4872

Cited by 132 publications

(71 citation statements)

References 17 publications

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“…Acquired spectra of cells/ tissues give a detailed biochemical fingerprint that varies dependent on the clinical status. It has been successfully applied in the study of various human tissues such as mineralized tissue (Kolovou and Anastasopoulou, 2007), skin (McIntosh, 1999), colon (Conti et al, 2008), breast (Anastassopoulou et al, 2009), arteries (Mamarelis et al, 2010), cartilage (Petra et al, 2005), the urinary tract (prostate, bladder) (Gazi etal., 2003), lung (Yano et al, 2000), liver (Li et al, 2004), heart and spleen (Chua-anusorn and Webb, 2000;Gough et al, 2003).…”

Section: Medical Applications Of Ft-ir Spectroscopymentioning

confidence: 99%

FT-IR Spectroscopy in Medicine

Dritsa¹

2012

Infrared Spectroscopy - Life and Biomedical Sciences

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Section: Medical Applications Of Ft-ir Spectroscopymentioning

confidence: 99%

FT-IR Spectroscopy in Medicine

Dritsa¹

2012

Infrared Spectroscopy - Life and Biomedical Sciences

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“…Technologies such as Fourier transform infrared (FTIR), Raman, and confocal fluorescence microscopy have been used to elucidate the origin and progression of cancer. [1][2][3][4][5][6] These technologies have also been used for the early detection of cancer and biochemical changes in cells and tissues causing cancer. 7,8 Among these spectroscopic techniques, Raman spectroscopy has attracted considerable attention for medical diagnosis since it is nondestructive, does not require a sample preparation process, and provides detailed information about the molecular structure of normal and diseased tissues.…”

Section: Introductionmentioning

confidence: 99%

Direct observation of spectral differences between normal and basal cell carcinoma (BCC) tissues using confocal Raman microscopy

et al. 2005

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“…These vibrational modes include stretching and bending that can occur in either a symmetric or asymmetric fashion (10,11). FTIR is a fast, efficient technique for collecting an infrared absorption spectrum over a broad spectral band with high sensitivity (12), and this technique has been used to identify neoplasia in breast (13), cervix (14), colon (15), lung (16), stomach (17), and pancreas (18). However, FTIR can be limited by the predominance of the water-absorption spectrum, and therefore, in this study, spectra are collected in ATR mode, which limits the influence of the water band.…”

mentioning

confidence: 99%

Detection of endogenous biomolecules in Barrett's esophagus by Fourier transform infrared spectroscopy

Wang

Triadafilopoulos²,

Crawford

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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Fourier transform infrared (FTIR) spectroscopy provides a unique molecular fingerprint of tissue from endogenous sources of light absorption; however, specific molecular components of the overall FTIR signature of precancer have not been characterized. In attenuated total reflectance mode, infrared light penetrates only a few microns of the tissue surface, and the influence of water on the spectra can be minimized, allowing for the analyses of the molecular composition of tissues. Here, spectra were collected from 98 excised specimens of the distal esophagus, including 38 squamous, 38 intestinal metaplasia (Barrett's), and 22 gastric, obtained endoscopically from 32 patients. We show that DNA, protein, glycogen, and glycoprotein comprise the principal sources of infrared absorption in the 950-to 1,800-cm ؊1 regime. The concentrations of these biomolecules can be quantified by using a partial leastsquares fit and used to classify disease states with high sensitivity, specificity, and accuracy. Moreover, use of FTIR to detect premalignant (dysplastic) mucosa results in a sensitivity, specificity, positive predictive value, and total accuracy of 92%, 80%, 92%, and 89%, respectively, and leads to a better interobserver agreement between two gastrointestinal pathologists for dysplasia ( ‫؍‬ 0.72) versus histology alone ( ‫؍‬ 0.52). Here, we demonstrate that the concentration of specific biomolecules can be determined from the FTIR spectra collected in attenuated total reflectance mode and can be used for predicting the underlying histopathology, which will contribute to the early detection and rapid staging of many diseases.dysplasia ͉ pathology ͉ optical biopsy ͉ diagnostics

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Direct Measurement of Human Lung Cancerous and Noncancerous Tissues by Fourier Transform Infrared Microscopy: Can an Infrared Microscope Be Used as a Clinical Tool?

Cited by 132 publications

References 17 publications

FT-IR Spectroscopy in Medicine

FT-IR Spectroscopy in Medicine

Direct observation of spectral differences between normal and basal cell carcinoma (BCC) tissues using confocal Raman microscopy

Detection of endogenous biomolecules in Barrett's esophagus by Fourier transform infrared spectroscopy

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