Direct Measurement of Pancreatic Enzymes After Stimulation With Secretin Versus Secretin Plus Cholecystokinin

Rosario, Del; Jf, Fitzgerald; Gupta, Sudhir; Jm, Croffie

doi:10.1097/00005176-200007000-00008

Cited by 22 publications

(22 citation statements)

References 17 publications

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“…The reason for the difference is not clear, and further clin− ical studies are needed to confirm the apparent usefulness of this protocol. Using direct (endoscopic) measurement of pancreatic enzymes (the "gold standard" for assessing exocrine function), Del Rosario and colleagues demonstrated that secretin was es− sentially as effective statistically as secretin plus cholecystokinin in provoking enzyme release [40]. Thus, although not commonly used as a marker of exocrine function, plasma amylase may be a convenient surrogate for more invasive testing procedures.…”

Section: Discussionmentioning

confidence: 99%

Secretin-stimulated Amylase Release into Blood is Impaired in Type 1 Diabetes Mellitus

Swislocki¹,

Noth²,

Hallstone³

et al. 2005

Horm Metab Res

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Patients with type 1 diabetes mellitus, but not type 2 diabetes mellitus, have reduced pancreatic exocrine function, supporting the concept of a local paracrine effect of insulin on pancreatic acinar cells. Further studies are needed to determine the clinical impact of this deficiency, and whether such patients with type 1 diabetes mellitus would benefit from therapy with pancreatic enzyme supplementation.

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Section: Discussionmentioning

confidence: 99%

Secretin-stimulated Amylase Release into Blood is Impaired in Type 1 Diabetes Mellitus

Swislocki¹,

Noth²,

Hallstone³

et al. 2005

Horm Metab Res

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“…It is well known that pancreatic enzyme secretion is stimulated by the cholinergic system and by cholecystokinin (Adler et al 1991;Del Rosario et al 2000). Treatment of the rats with carbamyl ␤-methylcholine, an analog of acetylcholine, led to decreases in levels of Cpn60 that coincided with decreases in pancreatic enzymes (Bendayan et al 1985).…”

Section: Discussionmentioning

confidence: 99%

Expression differences in mitochondrial and secretory chaperonin 60 (Cpn60) in pancreatic acinar cells

Gingras²,

Londono³

et al. 2003

Cell Stress Chaper

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In pancreatic acinar cells, chaperonin Cpn60 is present in all the cellular compartments involved in protein secretion as well as in mitochondria. To better understand the role Cpn60 plays in pancreatic secretion, we have evaluated its changes under experimental conditions known to alter pancreatic secretion. Quantitative protein A-gold immunocytochemistry was used to reveal Cpn60 in pancreatic acinar cells. Cpn60 immunolabelings in cellular compartments involved in secretion were found to decrease in acute pancreatitis as well as upon stimulation of secretion and in starvation conditions. A major increase in Cpn60 was recorded in diabetic condition. This was normalized by insulin treatment. Although in certain situations changes in secretory enzymes and in Cpn60 correlate well, in others, nonparallel secretion seemed to take place. In contrast, expression of mitochondrial Cpn60 in acinar cells appeared to remain stable in all conditions except starvation, where its levels decreased. Expression of Cpn60 in the secretory pathway and in mitochondria thus appears to behave differently, and Cpn60 in the secretory pathway must be important for quality control and integrity of secretion.

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“…Hopson et al 10.3389/fped.2022.908542 (129). Interpretation of the test results should be based on the sample with the highest (peak) enzyme activities (129,130).…”

Section: Methods Of Fluid Collection and Analysismentioning

confidence: 99%

“…There is a known dilutional effect of enzyme activities by ductal cell secretions if the fluid is collected beyond 10 min ( 129 ). Interpretation of the test results should be based on the sample with the highest (peak) enzyme activities ( 129 , 130 ).…”

Section: Direct (Stimulatory) Exocrine Function Testsmentioning

confidence: 99%

Assessment of exocrine pancreatic function in children and adolescents with direct and indirect testing

et al. 2022

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The exocrine pancreas plays an important role in digestion. Understanding of the physiology and regulation of exocrine function provides insight into disease processes and basis of functional testing. Specifically, exocrine pancreatic insufficiency (EPI) can cause maldigestion and thus a proper assessment of exocrine pancreatic function is important. There are indirect and direct methods for evaluating pancreatic function. Indirect methods are varied and include stool, serum, urine, and breath tests. Fecal elastase is a commonly used indirect test today. Direct methods involve stimulated release of pancreatic fluid that is collected from the duodenum and analyzed for enzyme activity. The most used direct test today is the endoscopic pancreatic function test. Indirect pancreatic function testing is limited in identifying cases of mild to moderate EPI, and as such in these cases, direct testing has higher sensitivity and specificity in diagnosing EPI. This review provides a comprehensive guide to indirect and direct pancreatic function tests as well as an in-depth look at exocrine pancreatic function including anatomy, physiology, and regulatory mechanisms.

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Direct Measurement of Pancreatic Enzymes After Stimulation With Secretin Versus Secretin Plus Cholecystokinin

Cited by 22 publications

References 17 publications

Secretin-stimulated Amylase Release into Blood is Impaired in Type 1 Diabetes Mellitus

Secretin-stimulated Amylase Release into Blood is Impaired in Type 1 Diabetes Mellitus

Expression differences in mitochondrial and secretory chaperonin 60 (Cpn60) in pancreatic acinar cells

Assessment of exocrine pancreatic function in children and adolescents with direct and indirect testing

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