2021
DOI: 10.3390/ijms22158240
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Direct Measurement of the Affinity between tBid and Bax in a Mitochondria-Like Membrane

Abstract: The execution step in apoptosis is the permeabilization of the outer mitochondrial membrane, controlled by Bcl-2 family proteins. The physical interactions between the different proteins in this family and their relative abundance literally determine the fate of the cells. These interactions, however, are difficult to quantify, as they occur in a lipid membrane and involve proteins with multiple conformations and stoichiometries which can exist both in soluble and membrane. Here we focus on the interaction bet… Show more

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Cited by 5 publications
(3 citation statements)
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References 81 publications
(147 reference statements)
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“…Similarly, BH3‐only Bcl‐2 members need multidomain Bcl‐2 members to trigger apoptosis (Renault et al, 2014). Indeed, binding of BH3‐only members with multidomain members is very essential for inducing apoptosis by stimulating structural alterations and other events that ultimately lead to the permeabilization of the outer mitochondrial membrane (Bloch et al, 2021; Rose et al, 2021). On the other hand, pro‐apoptotic BH3‐only members, such as p53 upregulated modulator of apoptosis (PUMA), bind anti‐apoptotic Bcl‐2 proteins neutralizing them and subsequently inducing apoptosis (Chen et al, 2018; Vavrova & Rezacova, 2014).…”
Section: Apoptosismentioning
confidence: 99%
“…Similarly, BH3‐only Bcl‐2 members need multidomain Bcl‐2 members to trigger apoptosis (Renault et al, 2014). Indeed, binding of BH3‐only members with multidomain members is very essential for inducing apoptosis by stimulating structural alterations and other events that ultimately lead to the permeabilization of the outer mitochondrial membrane (Bloch et al, 2021; Rose et al, 2021). On the other hand, pro‐apoptotic BH3‐only members, such as p53 upregulated modulator of apoptosis (PUMA), bind anti‐apoptotic Bcl‐2 proteins neutralizing them and subsequently inducing apoptosis (Chen et al, 2018; Vavrova & Rezacova, 2014).…”
Section: Apoptosismentioning
confidence: 99%
“…( x, y, z ) is the position of the pixel, where x = id and y = j d. ω 0 , ω z are the short and long 1/ e 2 radii of the Gaussian confocal detection volume, respectively. The average signal at each pixel was then calculated as the sum of the signal contributed by each particle, plus a constant background intensity I bckg (97). When needed, the effect of photon noise was taken into account by replacing, at each pixel, the calculated average signal by a value drawn from a Poisson noise with mean and variance equal to that average signal.…”
Section: Discussionmentioning
confidence: 99%
“…A critical step in apoptosis is the permeabilization of the outer-mitochondrial membrane, controlled by Bcl-2 family proteins. Binding and conformational dynamics of two main Bcl-2 family members, the pore-forming protein Bax and the truncated form of the activator protein Bid (tBid) are imaged and characterized at the single-particle level in a mitochondria-like planar lipid bilayer [27]. Comparable to apoptosis, ferroptosis is programmed cell death mediated by iron-dependent lipid peroxidation.…”
mentioning
confidence: 99%