Direct microtuber formation and enhanced growth in the acclimatization of in vitro plantlets of taro (Colocasia esculenta spp.) using hydroponics

Nhựt, Dương Tấn; Hương, Nguyễn Thị; Khiem, Dinh Van

doi:10.1016/j.scienta.2003.10.012

Cited by 15 publications

(8 citation statements)

References 3 publications

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“…Transferring the young plantlets to hydroponic culture before establishing in the soil enhanced the growth and increased the survival rate of the plantlets. The hydroponic system allows for normal, well-developed root growth, higher survival rate of the plants and is usually considered an acclimatization step before transferring to soil (Nhut et al 2004). In addition, hydroponically grown Taxus plants can be "milked" using hydropony and permeabilization (Gontier et al 2002).…”

Section: Resultsmentioning

confidence: 99%

Rapid germination and development of Taxus baccata L. by in vitro embryo culture and hydroponic growth of seedlings

Tafreshi

Shariati

Mofid

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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A highly promising procedure to obtain seedlings of Taxus baccata L. has been developed, which involves a combination of in vitro embryo culture and growth under hydroponic conditions. Embryos isolated from freshly collected seeds were 100% sterile, even though the seeds were not treated with acid or soaked in water prior to culturing. The embryo germination level of non-leached seeds was slightly lower (85%) than those leached in running water for 7 d (100%). The leached embryos germinated with extended roots while the non-leached embryos had abnormal shapes. The embryos cultured on media supplemented with an absorbent (PVP or activated charcoal) had extended roots and shoots and were a larger size without any browning, as compared to those grown without the supplement; activated charcoal gave better results. There were no significant differences in germination rates of T. baccata embryos between the media with differing strengths of macronutrients; however, for further development of the shoot, it was necessary to sub-culture the seedlings in MS in the light. To obtain seedlings with longer roots, they had to be maintained in one-half strength MS in darkness. Approximately 90% of the plants survived when grown hydroponically for 2 mo. The surviving plants showed well-extended roots and were a good starting material for genomic, proteomic, and conservational studies as well as Taxol permeabilization investigations.

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Section: Resultsmentioning

confidence: 99%

Rapid germination and development of Taxus baccata L. by in vitro embryo culture and hydroponic growth of seedlings

Tafreshi

Shariati

Mofid

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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“…The maximum survival rate of 100% was observed in hydroponic and coconut fiber cultures followed by those cultured in sand and soil, respectively. Nhut et al (2004) also showed that taro (Colocasia esculenta) plantlets cultivated in hydroponic system had higher survival rate (100%) than those cultivated in soil (85%). While, Kurtar et al (2010) reported that the lowest survival rate (12.5% -16.7%) of winter squash (Cucurbita maxima) and pumpkin (C. moschata) plantlets was found when cultivated in sand and soil.…”

Section: Effects Of Different Soilless Culture On Survival Rate and Gmentioning

confidence: 95%

“…In Heliconia bihai, the high survival rate of plantlets was found in sand and PlantMax substrates but low in vermiculite (Rodrigues et al, 2005). Nhut et al (2004) reported that the survival rate, plant height and leaves number of Colocasia esculenta plantlets cultivated in hydroponic were higher than those in soil culture. Moreover, microtuber formation was only found in the plantlets grown in hydroponics for 15 or 30 days.…”

Section: Effects Of Different Soilless Culture On Survival Rate and Gmentioning

confidence: 99%

Influence of Plantlet Age and Different Soilless Culture on Acclimatization of Stemona curtisii Hook.f.

Palee¹,

Dheeranupattana²,

Jatisatienr³

et al. 2012

Asian J. of Plant Sciences

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Influence of plantlet age and different soilless culture on acclimatization of Stemona curtisii Hook.f. AbstractThe aim of this experiment was to study the optimal age of Stemona curtisii plantlets for acclimatization. The in vitro shoots of S. curtisii were cultured on Murashige and Skoog solid medium supplemented with 1 mg L-1 naphthalene acetic acid to induce roots. Then, the plantlets from in vitro culture of 4, 8 and 12 week-old were transferred into the soil and their survival rate during the acclimatization process in the greenhouse was investigated. It was found that the 8 week-old plantlets had the highest survival rate of 80%. However, to enhance the survival rate of these plantlets, a soilless culture technique as a possible approach for the acclimatization was considered. Eight week-old plantlets were transferred into either, soil, a hydroponic system (nutrient film technique), coconut fiber or sand. The plantlets which were cultivated in coconut fiber or hydroponic culture showed 100% survival rate with the highest average number of new roots per plant. However, the mean root length of the plantlets grown in hydroponic system was significantly higher than that grown in coconut fiber. AbstractThe aim of this experiment was to study the optimal age of Stemona curtisii plantlets for acclimatization. The in vitro shoots of S. curtisii were cultured on Murashige and Skoog solid medium supplemented with 1 mg/L naphthalene acetic acid to induce roots. Then, the plantlets from in vitro culture of 4, 8 and 12 week-old were transferred into the soil and their survival rate during the acclimatization process in the greenhouse was investigated. It was found that the 8 week-old plantlets had the highest survival rate of 80%. However, to enhance the survival rate of these plantlets, a soilless culture technique as a possible approach for the acclimatization was considered. Eight week-old plantlets were transferred into either, soil, a hydroponic system (nutrient film technique), coconut fiber or sand. The plantlets which were cultivated in coconut fiber or hydroponic culture showed 100% survival rate with the highest average number of new roots per plant. However, the mean root length of the plantlets grown in hydroponic system was significantly higher than that grown in coconut fiber.2

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“…The reactor was operated at 44 ± 3˚C by a cooling roots [21]. Nowadays, hydroponics is considered as a promising technique not only for plant physiology experiments but also for commercial production [22,23]. Therefore, an application of this culture technique can be considered as an alternate approach for large-scale production of some desired and valuable crops.…”

Section: Production Of Liquid Fertilizermentioning

confidence: 99%

Complete reuse of raw fishmeal wastewater: Evidence from a field cultivation study and economic analysis

Kang

Jung

Kim

2018

Environmental Engineering Research

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A scaled-up bioconversion of fishmeal wastewater (FMW) into liquid fertilizer was performed five times in a 1 m 3 reactor in order to examine the feasibility of commercialization. The importance of aeration was marked. Analyses indicated that dissolved oxygen (DO) level was closely related to the value of oxidation-reduction potential (ORP) and it was crucial to achieve high-quality liquid fertilizer. When pure oxygen was supplied through four diffusers into the reactor, DO levels and ORP values were maintained over 1.2 mg/L and 0.2 mV, respectively all the time during 52 hr of bioconversion. The pH changed from 6.8 to 5.9. The average removal percentages of chemical oxygen demand (COD Cr ) and total nitrogen (TN) were 75.0% and 71.6%, respectively. Compared to the result acquired in a 5-L reactor, bioconversion of FMW into liquid fertilizer was achieved in a shorter time under the same removal percentages of COD Cr and TN. The 52-hr culture of inoculated FMW was phytotoxic-free and it possessed comparable fertilizing ability to a liquid fertilizer made from the fish waste in hydroponic culture with amino acid contents of 5.93 g/ 100 g sample. From all the above results, transferring labscale data to large-scale production appeared to be successful. As a result, the commercialization of a liquid fertilizer made from FMW was feasible.

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Direct microtuber formation and enhanced growth in the acclimatization of in vitro plantlets of taro (Colocasia esculenta spp.) using hydroponics

Cited by 15 publications

References 3 publications

Rapid germination and development of Taxus baccata L. by in vitro embryo culture and hydroponic growth of seedlings

Rapid germination and development of Taxus baccata L. by in vitro embryo culture and hydroponic growth of seedlings

Influence of Plantlet Age and Different Soilless Culture on Acclimatization of Stemona curtisii Hook.f.

Complete reuse of raw fishmeal wastewater: Evidence from a field cultivation study and economic analysis

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