Direct monitoring of ethanol in the brain

Rocchitta, Gaia; Serra, Pier Andrea

doi:10.13172/2053-0285-1-2-840

Cited by 3 publications

(8 citation statements)

References 33 publications

(37 reference statements)

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“…As previously reported [15], these techniques are particularly suitable for patients, as well as animal models, for their non-invasiveness. However, they are characterized by low spatial and temporal resolution, as well as a moderately high limit of detection [16].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the coupling with HPLC allowed the measurements of numerous compounds present in microsdialysates obtained from extracellular fluids [21][22][23]. Low temporal resolution, the relatively large probe size (∼200 µm), as well as the need for coupling with an analytical technique to quantify analytes in the microdialysate, are the fundamental limitations [16,24].…”

Section: Introductionmentioning

confidence: 99%

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A New Perspective on Using Glycols in Glutamate Biosensor Design: From Stabilizing Agents to a New Containment Net

et al. 2020

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Glutamate is a major excitatory neurotransmitter in the brain. It is involved in many normal physiological brain activities, but also neurological disorders and excitotoxicity. Hence, glutamate measurement is important both in clinical and pre-clinical studies. Pre-clinical studies often use amperometric biosensors due to their low invasiveness and the relatively small size of the devices. These devices also provide fast, real-time measurements because of their high sensitivity. In the present study, diethylene glycol (DEG), neopentyl glycol (NPG), triethylene glycol (TEG), and glycerol (GLY) were used to increase the long-term stability of glutamate biosensors. The evaluation was made by measuring variations of the main enzymatic (VMAX and KM) and analytical (Linear Region Slope (LRS)) parameters. Of the glycols tested, TEG was the most promising stabilizer, showing about twice as high VMAX maintained over a greater duration than with other stabilizers tested. It is also yielded the most stable linear region slope (LRS) values over the study duration. Moreover, we highlighted the ability of glycols to interact with enzyme molecules to form a containment network, able to maintain all the layered components of the biosensor adhering to the transducer.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A New Perspective on Using Glycols in Glutamate Biosensor Design: From Stabilizing Agents to a New Containment Net

et al. 2020

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“…Numerous techniques in animal models have been aimed at detecting ethanol concentration either in the whole brain or in discrete regions (Rocchitta & Serra, 2013). Among them, although mostly used to assess changes in neurotransmitters concentrations, in vivo brain microdialysis has been the most exploited.…”

Section: Introductionmentioning

confidence: 99%

“…This technique allows collecting information about neurochemicals in the extracellular space by means of the implantation of a semipermeable probe (300-mm outer diameter). Indeed, after over 30 years, brain microdialysis still represents a very powerful approach whose pros and cons have been elegantly and thoroughly discussed in seminal reviews (Di Chiara, Tanda, & Carboni, 1996;Kennedy, 2013;Rocchitta & Serra, 2013). In particular, brain microdialysis has proven to be slightly invasive and characterized by a relatively low temporal resolution, and characterized by requiring an association with an analytical instrumentation for the analysis of collected samples (Rocchitta & Serra, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, after over 30 years, brain microdialysis still represents a very powerful approach whose pros and cons have been elegantly and thoroughly discussed in seminal reviews (Di Chiara, Tanda, & Carboni, 1996;Kennedy, 2013;Rocchitta & Serra, 2013). In particular, brain microdialysis has proven to be slightly invasive and characterized by a relatively low temporal resolution, and characterized by requiring an association with an analytical instrumentation for the analysis of collected samples (Rocchitta & Serra, 2013). In an attempt to circumvent these limitations, in recent years different electrochemical devices have been developed, such as the amperometric biosensors (Rocchitta & Serra, 2013), that allow real-time monitoring of molecules present in the extracellular fluids of the brain with greater temporal resolution.…”

Section: Introductionmentioning

confidence: 99%

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