Settling of one or two large solid particles in a bioconvection flow induced by gyrotactic motile microorganisms is investigated using a 2D numerical model. The results of varying the initial positions of large particles on the bioconvection flow pattern are investigated. The Chimera method is utilized to generate subgrids around the moving particles. It is demonstrated that the introduction of a single large particle displaces bioconvection plume and changes its shape. The introduction of two particles on the same side of the bioconvection plume further displaces the plume while the introduction of two particles on opposite sides reduces this displacement. The influence of the bioconvection plume on the particles' settling paths and particles' settling velocities is investigated. Copyright © 2006 John Wiley & Sons, Ltd.