1993
DOI: 10.1002/bms.1200220307
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Direct observation of a DNA quadruplex by electrospray ionization mass spectrometry

Abstract: In 10 mM sodium phosphate, pH 7.6, containing 0.1 mM ethylenediaminetetraacetic acid, ions correspondings to the non-calent, four-stranded oligonucleotide, d(CGCG4GCG)4, were detected by negative ion electrospray ionization (ESI) mass spectrometry at a low nozzle-skimmer (delta NS) bias (-150 V), but not at a higher delta NS bias (> -250 V). In contrast, when the sample was desalted and analyzed by ESI mass spectrometry at a low delta NS bias only ions for the single-stranded d(CGCG4GCG) species were observed.… Show more

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Cited by 106 publications
(58 citation statements)
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“…Electrospray Ionization Mass Spectrometry (ESI-MS) was successfully employed for detection a G-quadruplex structure formation in 1993 (Goodlett et al, 1993). Since then the technique has been a useful tool in structural studies of G-quadruplexes, including strand stoichiometry (Joly et al, 2012;Borbone et al, 2011;Cui and Yuan, 2011;Rosu et al, 2002 andZhou et al, 2007), quadruplex-ligand interactions Li et al, 2008;Gabelica et al, 2007), structural transitions Amato et al, 2009 andLi et al, 2009), the kinetics of structure formation (Rosu et al, 2010;Gabelica et al, 2009) and many others (for a review, see Yuan et al, 2011).…”
Section: Electrospray Ionization Mass Spectrometry (Esi-ms)mentioning
confidence: 99%
“…Electrospray Ionization Mass Spectrometry (ESI-MS) was successfully employed for detection a G-quadruplex structure formation in 1993 (Goodlett et al, 1993). Since then the technique has been a useful tool in structural studies of G-quadruplexes, including strand stoichiometry (Joly et al, 2012;Borbone et al, 2011;Cui and Yuan, 2011;Rosu et al, 2002 andZhou et al, 2007), quadruplex-ligand interactions Li et al, 2008;Gabelica et al, 2007), structural transitions Amato et al, 2009 andLi et al, 2009), the kinetics of structure formation (Rosu et al, 2010;Gabelica et al, 2009) and many others (for a review, see Yuan et al, 2011).…”
Section: Electrospray Ionization Mass Spectrometry (Esi-ms)mentioning
confidence: 99%
“…It is not disrupted by guanidine hydrochloride, pH extremes, SDS, reducing agents or heat and it is resistant to many proteases [14]. Electrospray ionization mass spectrometry (ESMS) [15] has previously been used to characterise non-covalent receptor-ligand complexes [16], oligonucleotide associations [17,18], non-covalent protein-peptide complexes [19] and more recently to study the tetramers formed by avidin [20], streptavidin [21], hemoglobin [20] as well as yeast alcohol dehydrogenase and rabbit muscle pyruvate kinase [22]. However, the use of ESMS is usually limited to mass measurements below 150 kDa due to the difficulty of resolving multiply charged ions at higher mass.…”
Section: Introductionmentioning
confidence: 99%
“…In a 10 mM NH4OAc solution, I by itself predominantly gave quadruplex ions in the ESI-MS spectra (data not shown). Intact quadruplexes have been previously studied by ESI-MS (41), and the GGGG at the middle of the sequence has been suggested to be responsible for the formation of the quadruplex (42). The oligonucleotide was denatured by heating at 80°C for 5 min followed by quick cooling and the resultant ESI-MS spectrum showed only monomeric ions (Fig.…”
mentioning
confidence: 99%