Direct-on-Target Microdroplet Growth Assay Applications for Clinical Antimicrobial Susceptibility Testing

Li, Rongrong; Tang, Hao; Xu, Huaming; Ren, Yingli; Li, Shujin; Shen, Jilu

doi:10.2147/idr.s303187

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…Recent advances in technology have relied on building devices using microfluidics and lab-on-a-disc systems with capabilities to test the growth of up to 100 bacterial strains within droplets ( 57 – 59 ). Another approach uses advances in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to rapidly screen functional sets of antibiotic resistance by direct-on-target microdroplet growth assays at a scale of 96 samples per assay ( 27 , 60 ). The current challenge these assays face is increasing throughput and accessibility for routine testing.…”

Section: Antibiotic Resistancementioning

confidence: 99%

High-throughput functional trait testing for bacterial pathogens

Stromberg,

Phillips,

Omberg

et al. 2023

mSphere

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Functional traits are characteristics that affect the fitness and metabolic function of a microorganism. There is growing interest in using high-throughput methods to characterize bacterial pathogens based on functional virulence traits. Traditional methods that phenotype a single organism for a single virulence trait can be time consuming and labor intensive. Alternatively, machine learning of whole-genome sequences (WGS) has shown some success in predicting virulence. However, relying solely on WGS can miss functional traits, particularly for organisms lacking classical virulence factors. We propose that high-throughput assays for functional virulence trait identification should become a prominent method of characterizing bacterial pathogens on a population scale. This work is critical as we move from compiling lists of bacterial species associated with disease to pathogen-agnostic approaches capable of detecting novel microbes. We discuss six key areas of functional trait testing and how advancing high-throughput methods could provide a greater understanding of pathogens.

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Section: Antibiotic Resistancementioning

confidence: 99%

High-throughput functional trait testing for bacterial pathogens

Stromberg,

Phillips,

Omberg

et al. 2023

mSphere

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show abstract

“…At varying antibiotic concentrations, the microbial growth in each of the target plates was examined to see if MALDI-TOF MS could successfully identify each microbial spot. Microbes could be classified as sensitive or resistant, and minimal inhibitory concentration (MIC) could be computed based on the antibiotic concentration in each microdroplet [ 26 ].…”

Section: Reviewmentioning

confidence: 99%

Unraveling the Nature of Antibiotics: Is It a Cure or a New Hurdle to the Patient Treatment?

Gude¹,

Gopal²,

Ramesh³

et al. 2022

Cureus

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“…The recently developed DOT-MGA allows for rapid universal antimicrobial susceptibility testing using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). 5,[8][9][10] Preliminary tests have shown the desirability of direct application of this approach in positive blood cultures and its good performance in detection. [11][12][13] The inoculum size of 5 × 10 5 CFU/mL, recommended by the CLSI 15 and ISO 16 guidelines as initial inoculum size for AST, is below the lower detection limit by MS.…”

Section: Introductionmentioning

confidence: 99%