Direct or DNA Extraction-Free Amplification and Quantification of Foodborne Pathogens

Williams, Maggie R.; Hashsham, Syed A.

doi:10.1007/978-1-4939-9000-9_2

Cited by 2 publications

(1 citation statement)

References 39 publications

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“…Bacterial genomes require additional attention in the design of robust oligonucleotide probes. This is due to the low GC content and complexity in sequence composition, as well as common conserved repeats [ 65 , 66 ]. In general, the drawbacks associated with DNA or PCR probes include uncontrolled cross-hybridization on repeats, unpredicted secondary structures, or partial homology among regions of PCR probes.…”

Section: Dna Probe Designmentioning

confidence: 99%

Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

Vidić

Vizzini

Manzano

et al. 2019

Sensors

104

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Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production. This review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification.

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Section: Dna Probe Designmentioning

confidence: 99%

Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

Vidić

Vizzini

Manzano

et al. 2019

Sensors

104

View full text Add to dashboard Cite

show abstract

Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications

Zhuang,

Gong,

Zhang

et al. 2024

Discover Nano

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Salmonella , the prevailing zoonotic pathogen within the Enterobacteriaceae family, holds the foremost position in global bacterial poisoning incidents, thereby signifying its paramount importance in public health. Consequently, the imperative for expeditious and uncomplicated detection techniques for Salmonella in food is underscored. After more than two decades of development, loop-mediated isothermal amplification (LAMP) has emerged as a potent adjunct to the polymerase chain reaction, demonstrating significant advantages in the realm of isothermal amplification. Its growing prominence is evident in the increasing number of reports on its application in the rapid detection of Salmonella . This paper provides a systematic exposition of the technical principles and characteristics of LAMP, along with an overview of the research progress made in the rapid detection of Salmonella using LAMP and its derivatives. Additionally, the target genes reported in various levels, including Salmonella genus, species, serogroup, and serotype, are summarized, aiming to offer a valuable reference for the advancement of LAMP application in Salmonella detection. Finally, we look forward to the development direction of LAMP and expect more competitive methods to provide strong support for food safety applications.

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Direct or DNA Extraction-Free Amplification and Quantification of Foodborne Pathogens

Cited by 2 publications

References 39 publications

Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications

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