Direct plant gene delivery with a poly(amidoamine) dendrimer

Pasupathy, Karthikeyan; Lin, Sijie; Hu, Qian; Luo, Hong; Ke, Pu Chun

doi:10.1002/biot.200800021

Cited by 66 publications

(36 citation statements)

References 24 publications

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“…However, protoplast-based transformation methods hold disadvantages in that the viability of the protoplasts and their ability to divide are strongly reduced by the chemicals that are applied to disorganize the cell wall. For this reason, recent studies have focused on intact plant cells; it was found to that they are able to achieve endocytosis to directly internalize single-walled carbon nanotubes (CNTs), CdSe/ZnS QDs, or poly (amidoamine) dendrimer from the extracellular environment [14][15][16][17][18]. Other work showed that multi-walled CNTs with attached cellulose are also able to penetrate the cell wall, and transport intracellularly through cellulose-inducing nanoholes [19].…”

mentioning

confidence: 99%

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Xia

Dong

Zhang

et al. 2012

Sci. China Life Sci.

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The characteristics of the interactions co-cultures of ultrafine mesoporous silica nanoparticles (MSNs) and the Liriodendron hybrid suspension cells were systematically investigated using laser scanning confocal microscope (LSCM) and scanning electron microscopy (SEM). Using fluorescein isothiocyanate (FITC) labeling, the LSCM observations demonstrated that MSNs (size, 5-15 nm) with attached FITC molecules efficiently penetrated walled plant cells through endocytic pathways, but free FITC could not enter the intact plant cells. The SEM measurements indicated that MSNs readily aggregated on the surface of intact plant cells, and also directly confirmed that MSNs could enter intact plant cells; this was achieved by determining the amount of silicon present. After 24 h of incubation with 1.0 mg mL 1 of MSNs, the viability of the plant cells was analyzed using fluorescein diacetate staining; the results showed that these cells retained high viability, and no cell death was observed. Interestingly, after the incubation with MSNs, the Liriodendron hybrid suspension cells retained the capability for plant regeneration via somatic embryogenesis. Our results indicate that ultrafine MSNs hold considerable potential as nano-carriers of extracellular molecules, and can be used to investigate in vitro gene-delivery in plant cells. Along with developments in the application of nanotechnology from animal science and medical research to plant science research, the impact of engineered nanomaterials on plant systems has attracted increasing attention, the areas including (i) the delivery of fertilizers, herbicides, pesticides and exogenous genes, (ii) the improvement of the growth of plants, and (iii) nanotoxicity research for plant cells [1][2][3][4][5][6][7][8][9][10]. However, compared with mammalian cells, the plant cell wall which is composed of cross-linked polysaccharides (cellulose, hemicelluloses, and pectin) represents an extra barrier surrounding the cell membrane that hinders the passage of nanoparticles into plant cells. To avoid the inhibiting effects of the plant cell wall, free protoplasts (which are prepared via the removal of the cell wall using cellulase treatments) have been used previously to study the internalization of various nanoparticles (e.g., CdSe/ZnS quantum dots (QDs), polystyrene nanospheres, poly(phenylene ethynelene) nanoparticles, and carbon nanomaterials) [11][12][13]. However, protoplast-based transformation methods hold disadvantages in that the viability of the protoplasts and their ability to divide are strongly reduced by the chemicals that are applied to disorganize the cell wall. For this reason, recent studies have focused on intact plant cells; it was found to that they are able to achieve endocytosis to directly internalize single-walled carbon nanotubes (CNTs), CdSe/ZnS QDs, or poly (amidoamine) dendrimer from the

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mentioning

confidence: 99%

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Xia

Dong

Zhang

et al. 2012

Sci. China Life Sci.

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“…Such conditions will facilitate the removal of gas hydrate blockages in the oil pipeline. Our study can also be applied to the general case of loading hydrophobic molecules onto branched polymers, such as encapsulating molecules inside a dendrimer for cell trafficking [31,32] and gene delivery [33]. Another extension from this research lies in our fundamental understanding of the diffusion and reptation of nonlinear polymers [34,35].…”

Section: Resultsmentioning

confidence: 94%

Single-Molecule Dendrimer-Hydrocarbon Interaction

Pasupathy¹,

Suek²,

Lyons³

et al. 2009

TONANOJ

Self Cite

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Abstract:We report our single-molecule fluorescence microscopy and molecular dynamics simulation studies on the interaction of poly(amidoamine) dendrimer and squalane hydrocarbon in aqueous solution. Our spectrophotometry measurements indicate that this interaction increases with the pH of the solvent. Our simulations show that squalane resides primarily on the perimeter of the dendrimer at low to neutral pH, but becomes encapsulated by the dendrimer at high pH. Using single-molecule fluorescence microscopy, we have identified that the binding between PAMAM and squalane is reversible. At a pH value of 8, the approaching, binding, and characteristic times of a single fluorescently-labeled dendrimer to squalane are 0.5 s, 7.5 s, and 0.5 s, respectively. Both our spectrophotometry measurements and simulations show that the interaction between PAMAM and squalane is stronger for lower generation dendrimers. This study facilitates our understanding of using dendritic and hyperbranched polymers for gas hydrate prevention in the petroleum industry.

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“…In an another paper Martin-Ortigosa et al [295] reported that the biolistic delivery of mesoporous SiO 2 -based nanomaterials can be improved by increasing the density of mesoporous SiO 2 -based NP through Au plating, and enhancement of the NP-mediated DNA delivery can be obtained using DNA-coating protocol based on calcium chloride and spermidine for mesoporous SiO 2 NPs and Au nanorods. Nanosized poly(amidoamine) dendrimer was used for direct and non-invasive delivery of green fluorescent protein-encoding plasmid DNA to turfgrass cells by Pasupathy et al [296]. Kogure [297] reported about development of a novel artificial gene delivery system -multifunctional envelope-type nano device for gene therapy.…”

Section: Dna and Gene Delivery Using Nanoparticlesmentioning

confidence: 99%

Application Of Nanotechnology In Agriculture And Food Industry, Its Prospects And Risks

Jampílek

Kráľová

2015

Ecological Chemistry and Engineering S

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Nanoagrochemicals, such as nanopesticides, nanofertilizers or plant growth stimulating nanosystems, were primarily designed to increase solubility, enhance bioavailability, targeted delivery, controlled release and/or protection against degradation resulting in the reduced amount of applied active ingredients and finally in a decrease of dose-dependent toxicity/burden. This paper is a comprehensive up-to-date review related to the preparation and the biological activity of nanoformulations enabling gradual release of active ingredient into weeds and the body of pests and controlled release of nutrients to plants. The attention is also devoted to the decrease of direct environmental burden and economic benefits due to application of nanoformulations, where less amount of active ingredient is needed to achieve the same biological effect in comparison with bulk. The application of nanotechnology in the areas such as food packaging, food security, encapsulation of nutrients and development of new functional products is analysed. The use of nanoparticles in biosensors for detection of pathogens and contaminants as well as in DNA and gene delivery is discussed as well. Benefits and health risks of nanoagrochemicals are highlighted, and special attention is given to nanoecotoxicology and guidelines and regulatory documents related to the use of nanoformulations in agriculture and food industry.

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Direct plant gene delivery with a poly(amidoamine) dendrimer

Cited by 66 publications

References 24 publications

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Single-Molecule Dendrimer-Hydrocarbon Interaction

Application Of Nanotechnology In Agriculture And Food Industry, Its Prospects And Risks

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