In concrete structures, replacing conventional steel bars with high-strength steel reinforcement (HSSR) can effectively save the use of materials. However, the deformation properties and strength dispersion of HSSR are different from those of conventional steel reinforcement, which restricts or conservatively uses them in practical applications. For example, the partial safety factor of HRB500 grade steel bars (the yield strength is 500 MPa) in guideline GB50010-2010 is larger than that of conventional steel bars, and there is no relevant guidance for HRB600 grade steel bars (the yield strength is 600 MPa). Based on this, this paper will propose the limit state design method of high-strength steel reinforced concrete beam (HSSRCB) based on reliability analysis, which is convenient for the popularization and use of HSSR. Firstly, the flexural performance test of HSSRCBs was introduced, and the flexural capacity of HSSRCB was analyzed based on the existing prediction model. Second, a sectional numerical analysis model was established, where the section was discretized into several points, and then the curvature was gradually increased to obtain the corresponding bending moment through integration. A large number of samples were calculated to obtain statistical characteristics of the error of prediction model. Then, the limit state functions were established for two kinds of format, including partial safety factor format (PSSF) and resistance reduction factor format (RRFF), respectively, and the reliability of HSSRCBs was analyzed based on Monte Carlo simulation. Finally, the recommended values of partial safety factor of material and reduction factor of bearing capacity were proposed, in which the design strength of HRB500 and HRB600 reinforcement was 454 MPa and 545 MPa for PSSF, respectively, and the resistance reduction factor for the flexural capacity of HSSRCB was 0.8 and 0.75 for RRFF, respectively.