2012
DOI: 10.1111/j.1750-3841.2012.02777.x
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Direct Quantification and Distribution of Tetracycline‐Resistant Genes in Meat Samples by Real‐Time Polymerase Chain Reaction

Abstract: :  The evolution of antimicrobial‐resistant bacteria has become a threat to food safety and methods to control them are necessary. Counts of tetracycline‐resistant (TR) bacteria by microbiological methods were compared with those obtained by quantitative PCR (qPCR) in 80 meat samples. TR Enterobacteriaceae counts were similar between the count plate method and qPCR (P= 0.24), whereas TR aerobic mesophilic bacteria counts were significantly higher by the microbiological method (P < 0.001). The distribution of t… Show more

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Cited by 5 publications
(3 citation statements)
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“…This result is in accordance with other authors who also found the presence of tet (A) more frequently than tet (B) in porcine E. coli [27,28], E. coli isolated from foods of animal origin [29], E. coli isolated from food animals [30] and Gram-negative clinical isolates [31]. Guarddon et al [32] also detected more tet (A) genes that tet (B) in samples of meat. However, it is necessary to highlight that the data presented in this work were obtained from the total microbiota in the baby food samples rather than from isolates, which offers a complete perspective on the amount of bacteria that harbor these genes.…”
Section: Resultssupporting
confidence: 89%
“…This result is in accordance with other authors who also found the presence of tet (A) more frequently than tet (B) in porcine E. coli [27,28], E. coli isolated from foods of animal origin [29], E. coli isolated from food animals [30] and Gram-negative clinical isolates [31]. Guarddon et al [32] also detected more tet (A) genes that tet (B) in samples of meat. However, it is necessary to highlight that the data presented in this work were obtained from the total microbiota in the baby food samples rather than from isolates, which offers a complete perspective on the amount of bacteria that harbor these genes.…”
Section: Resultssupporting
confidence: 89%
“…Meat samples Aarestrup, 2003) or gram-negative clinical isolates (Fan, Hamilton, Webster-Sesay, Nikolich, & Lindler, 2007). Guarddon, Miranda, Vázquez, Cepeda, and Franco (2012) also detected more tet(A) genes than tet(B) in conventional meat. Nevertheless, these rates changed regarding the LOD of the genes.…”
Section: Production Methodsmentioning
confidence: 98%
“…Additionally, with the aid of qPCR, resistance genes have been investigated both in goat and sheep meat as well as in the slaughterhouse environment. Researchers confirmed the presence of tetA and tetB genes in several areas of the slaughterhouse as well as in meat products [ 62 ]. Using qPCR, it was possible for researchers to identify the presence of resistance genes in other types of meat product; tetA and tetB genes were found in these different samples [ 37 ].…”
Section: Molecular Methodsmentioning
confidence: 99%