Direct reaction of taurine with malondialdehyde: evidence for taurine as a scavenger of reactive carbonyl species

Li, Guolin; Tang, Ting; Peng, Mijun; He, Hua; Yin, Dong

doi:10.1179/135100010x12826446921743

Cited by 14 publications

(10 citation statements)

References 32 publications

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“…This process is associated with aging and age‐related diseases, especially in the formation of lipofuscin and AGEs 18,31 . Our previous studies determined that a variety of amino compounds (such as pyridoxamine, glucosamine, and taurine) can directly capture MDA and inhibit the formation of lipofuscin‐like fluorophores in proteins modified by MDA 32‐35 …”

Section: Discussionmentioning

confidence: 99%

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Metformin scavenges formaldehyde and attenuates formaldehyde‐induced bovine serum albumin crosslinking and cellular DNA damage

Mai

Zhou

Lin

et al. 2020

Environmental Toxicology

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Formaldehyde (FA) can be produced in the environment and by cell metabolism and has been classified as a carcinogen in animals and humans. Metformin is the most commonly used drug for the treatment of type 2 diabetes. Metformin also has potential benefit in cancer prevention and treatment. The aim of this study was to determine whether metformin can directly react with FA and attenuate its toxicity in vitro. Metformin was incubated at pH 7.4 and 37 C in the presence of FA, and the reaction mixture was analyzed by UV spectrophotometry, high-performance liquid chromatography (HPLC), and mass spectrometry. Fluorescence spectrophotometry, immunofluorescence, and western blot were used to measure FA-induced bovine serum albumin (BSA) crosslinking and DNA damage in HepG2 cells treated with or without metformin. According to the HPLC and mass spectrometry data, we speculate that the reaction of metformin with FA (1:1) initially results in the formation of a conjugated intermediate followed by the subsequent generation of a stable six-membered ring structure. Correspondingly, metformin attenuated FA-induced fluorescence in BSA as well as the aggregation of γH2AX in HepG2 cells. These results suggest that metformin can protect protein and DNA damage induced by FA at least partly through a direct reaction process.

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Section: Discussionmentioning

confidence: 99%

“…Second step, a stable six-ringed product (final product) was obtained slowly by dehydration MDA. [32][33][34][35] In this study, we used UV spectrophotometry and HPLC to show that metformin can react with FA. Further mass spectrometry results…”

Section: Immunofluorescencementioning

confidence: 99%

Metformin scavenges formaldehyde and attenuates formaldehyde‐induced bovine serum albumin crosslinking and cellular DNA damage

Mai

Zhou

Lin

et al. 2020

Environmental Toxicology

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“…Being an antioxidant, taurine also has the ability to scavenge reactive oxygen particles (Huxtable ), attenuate the production of MDA (the most abundant individual aldehydic decomposition product of autoxidized polyunsaturated fatty acid, an indicator of lipid peroxidation) (Li et al ; Fang et al ), and consequently stabilize the biological membrane (Wu et al ). It was reported that taurine could reduce an animal's response to oxidative stress, such as metal‐induced toxicosis (Gürer et al ; Sinha et al ), aging (Yildirim and Kilic ) and other diseases (Schaffer et al ).…”

Section: Discussionmentioning

confidence: 99%

Effects of Taurine Supplementation on Growth Performance and Antioxidative Capacity of Chinese Soft‐shelled Turtles, Pelodiscus sinensis, Fed a Diet of Low Fish Meal Content

JinLiang

Jia

Yang

et al. 2013

J World Aquaculture Soc

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This experiment examined the effects of taurine supplementation on the growth performance and antioxidative capacity of Chinese soft-shelled turtle, Pelodiscus sinensis, fed diets of low fish meal content. The base diet (with 0.31% taurine) consisted of low fish meal level and had an animal : plant protein ratio of 2:1. Six experimental diets were supplemented with various taurine levels (0, 0.25, 0.5, 0.75, 1.0, and 1.25%) for 8 wk. There were significant quadratic regression relationships between the taurine supplementation level and specific growth rate (SGR), feed conversion ratio (FCR), and protein efficiency ratio (PER). All taruine supplemented groups showed higher SGR and PER and lower FCR than the group fed diet without taurine supplementation. Significant quadratic regressions were also showed between the supplemented taurine level and the total superoxide dismutase activity, malondialdehyde content, and total antioxidant capacity. The results indicate that taurine supplementation in a diet with low fish meal level can improve the growth of turtles. With comprehensive analysis on the effects of taurine supplementation on the growth performance and antioxidative capacity of turtles, the optimal taurine supplementation level was suggested to be 0.9% in the diet with an animal : plant protein ratio of 2:1.

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“…There are some reports regarding short-term antioxidant treatments able to reduce lipid peroxidation after AMI and reperfusion [3,29]; moreover, antioxidants seem to have a cardioprotective action in patients with chronic coronary artery disease [19]. As regards protein oxidation, there are data about the carbonyl scavenger activity played by some molecules [10,15,20]; in particular L-arginine significantly reduced the level of protein oxidation in ischemic heart disease [27,28].…”

Section: Conclusive Considerationsmentioning

confidence: 99%

Behaviour of protein carbonyl groups in juvenile myocardial infarction

Caimi

Canino

Incalcaterra

et al. 2013

Clinical Hemorheology and Microcirculation

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Acute myocardial infarction (AMI) is accompanied by oxidative stress, and protein oxidation is among the consequences of oxidative stress. We examined the plasma concentration of protein carbonyl groups (PC), a marker of protein oxidation, in a group of young subjects with AMI (45 men and 5 women; mean age 40.4 ± 4.8 yrs). We found a significant increase of PC (p < 0.001) in comparison with normal controls. No difference was observed between patients with AMI characterized by elevated ST segment and those without elevation of ST segment. There was no correlation between the ejection fraction and PC in the whole group nor in the subgroups of STEMI and non-STEMI patients. Subdividing the whole group of AMI patients according to the number of risk factors and the number of stenosed coronary vessels, the difference in PC level was not statistically significant among the subgroups. This study showed an increased protein oxidation in young subjects with recent AMI. Further investigation is needed to ascertain whether this can be a target of therapeutic intervention.

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Direct reaction of taurine with malondialdehyde: evidence for taurine as a scavenger of reactive carbonyl species

Cited by 14 publications

References 32 publications

Metformin scavenges formaldehyde and attenuates formaldehyde‐induced bovine serum albumin crosslinking and cellular DNA damage

Metformin scavenges formaldehyde and attenuates formaldehyde‐induced bovine serum albumin crosslinking and cellular DNA damage

Effects of Taurine Supplementation on Growth Performance and Antioxidative Capacity of Chinese Soft‐shelled Turtles, Pelodiscus sinensis, Fed a Diet of Low Fish Meal Content

Behaviour of protein carbonyl groups in juvenile myocardial infarction

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