Abstract:The direct reagentless electrochemical detection of recombinant firefly luciferase binding with gold electrode modified with nickel ions has been carried out. Self-assembly of molecules of functional disulfide containing chelating moieties on the gold electrode was monitored by contact angle measurements and confirmed by electrochemical data as three times decrease of electric double layer capacitance. Succeeding nickel ions chelating followed by selective binding of target protein were registered by reagentle… Show more
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