Direct regeneration and genetic fidelity analysis of regenerated plants of Andrographis echioides (L.) - An important medicinal plant

Savitikadi, Pandarinath; Jogam, Phanikanth; Rohela, Gulab Khan; Ellendula, Raghu; Sandhya, Dulam; Allini, Venkateswar Rao; Abbagani, Sadanandam

doi:10.1016/j.indcrop.2020.112766

Cited by 43 publications

(17 citation statements)

References 21 publications

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“…Morphogenic responses, such as shoot regeneration, are observed by the application of cytokinins either alone (Razaq et al, 2012;Saha et al, 2014;Sadeghi et al, 2015), or in combination with auxins (Upadhyay et al, 2015;Savitikadi et al, 2020). Both PGRs have a synergistic effect on morphogenic responses, which has been reported in other medicinal species such as Rhodiolarosea (Dimitrov et al, 2003).…”

Section: In Vitro Culture Shoot Development and Multiplication Indexmentioning

confidence: 90%

Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona officinalis

2021

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Cinchona officinalis is an important species from the Andean cloud forest that has a low regeneration rate in natural populations. In vitro regeneration of C. officinalis has been successfully established but somaclonal variation was not evaluated. The regeneration pathway and the number of subcultures on somaclonal variation were evaluated using six ISSR primers that amplified 58 loci of Inter Simple Sequence Repeats (ISSR). A dendrogram based on Jaccard´s genetic distance between the subcultures and the donor plant was produced. The results show that indirect shoot regeneration induces somaclonal variation, in the presence 2,4-Dichlorophenoxyacetic acid (2,4-D) in combination with kinetin and 6-Benzylaminopurine (BAP). In combination with 1-Naphthaleneacetic acid (NAA) or with Indole-3-butyric acid (IBA), BAP produces genetically stable explants. The highest proliferation rate was achieved using BAP and IBA. The present research study suggests avoiding the use of 2,4-D when C. officinalis is propagated for reintroduction and restoration projects.

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Section: In Vitro Culture Shoot Development and Multiplication Indexmentioning

confidence: 90%

Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona officinalis

2021

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“…Similar genetic fidelity analysis using trnH-psbA and rbcL barcode primes was reported in Dioscorea spp. (Adeniran et al 2018), Artemisia vulgaris L. (Jogam et al 2020), and Andrographis echioides, an Acanthaceae member (Savitikadi et al 2020). DNA barcode primers are used in differentiating species identity and genetic diversity.…”

Section: Total Phenolics Flavonoids and Antioxidant Potentialmentioning

confidence: 99%

In vitro regeneration, antioxidant potential, and genetic fidelity analysis of Asystasia gangetica (L.) T.Anderson

Dilkalal

Akula

Umesh

2021

In Vitro Cell.Dev.Biol.-Plant

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An effective protocol for the plant regeneration via direct and indirect organogenesis has been developed from leaf explants of Asystasia gangetica (L.), cultured on Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of auxin and cytokinins. Approximately 86% of explants produced direct shoots on MS medium containing 0.5 mg L −1 6-benzyladenine (BA) and 10 μg L −1 Triacontanol (TRIA) with a maximum of 4.82 ± 0.29 shoots per leaf segment. For production of callus-mediated plantlets (indirect), primarily callus was induced on MS medium containing 2 mg L −1 2,4dichlorophenoxyacetic acid (2,4-D), which was then subcultured on medium with 0.1 mg L −1 naphthaleneacetic acid (NAA), 0.5 mg L −1 BA, and 1 to 8 mg L −1 2-isopentenyl adenine (2iP) in order to develop organogenic callus and subsequent shoot induction. A maximum of 6.84 ± 0.05 shoots per callus clump was obtained on MS media supplemented with 4 mg L −1 2iP, 0.5 mg L −1 BA, and 0.1 mg L −1 NAA. The shootlets produced roots when cultured on half-strength MS media supplemented with 2 mg L −1 indole-3-butyric acid (IBA). In vitro propagated plantlets were hardened on soil rite and acclimatized to field condition with 85% survivability. The chlorophyll content of acclimatized plants was comparable with that of the mother plant, while stomatal micromorphology of regenerated plants exhibited no abnormalities. The radical scavenging and antioxidant activity of methanolic extract of leaves were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing ability of plasma (FRAP), and phosphomolybdenum test. In all experiments, regenerated plants exhibited enhanced antioxidant potential indicating micropropagated plants could be exploited for isolation of novel biomolecules. Further, the genetic homogeneity of acclimatized plants was confirmed by PCR-based start codon targeted (SCoT) markers and ycf1b DNA barcoding primers which exhibited monomorphic bands identical to the normal mother plant and no variations were observed.

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“…However, these earlier reports involved different cultivars with different physiological conditions and may be due to that the findings of Chapman [ 37 ] are not in match with the present study which could not establish the rooting with the IBA and IAA. A similar type of higher number of root induction on combination of IAA and IBA was achieved in Mirabillis jalapa [ 38 ] and Andrographis echioides [ 39 ].…”

Section: Discussionmentioning

confidence: 62%

Effect of Growth Regulators on In Vitro Micropropagation of Potato (Solanum tuberosum L.) Gudiene and Belete Varieties from Ethiopia

Hajare

Chauhan

Kassa

2021

The Scientific World Journal

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Aim. Potato (Solanum tuberosum L.) is one of the important crops in Ethiopia which has a crucial role in nutritional security, poverty alleviation, and income generation. The aim of the present investigation is to develop an efficient in vitro propagation protocol for Belete and Gudiene potato varieties by using lateral bud as explants. Materials and Methods. Shoot initiation was achieved by inoculating buds on full-strength MS Murashige and Skoog medium (MS) fortified with variable concentrations of BAP and NAA. Basal MS was used as control throughout the experiment. Results. Results of our study showed that best shoot initiation was obtained on MS medium supplemented with 1.5 mg/l BAP + 3.0 mg/l NAA for Gudiene variety, whereas 1.0 mg/l BAP and 2.0 mg/l NAA produced more shoots in Belete variety. The initiated shoots increased two- to three-fold upon subculture on the MS medium fortified with varying concentrations of BAP and Kinetin. The highest numbers of multiple shoots were obtained in the MS medium containing 2.5 mg/l Kinetin. The combined effect of BAP and Kinetin did not produce any additional positive effect for shoot multiplication. Rooting percentage and number of roots/shoot were found best on the MS medium fortified with 1.0 mg/l IBA + 0.5 IAA. Conclusions. The variety Gudiene was found best for shoot initiation and root formation, while Belete variety proved its superiority for multiple shoot formation. A total number of 82.66% of plantlets were acclimatized under field conditions. This work indicates the practical applicability of plant tissue culture using lateral bud as explants is effective for micropropagation of potato in vitro.

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Direct regeneration and genetic fidelity analysis of regenerated plants of Andrographis echioides (L.) - An important medicinal plant

Cited by 43 publications

References 21 publications

Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona officinalis

Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona officinalis

In vitro regeneration, antioxidant potential, and genetic fidelity analysis of Asystasia gangetica (L.) T.Anderson

Effect of Growth Regulators on In Vitro Micropropagation of Potato (Solanum tuberosum L.) Gudiene and Belete Varieties from Ethiopia

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