DIRECT RENAL ACTION OF A PURIFIED PARATHYROID EXTRACT<sup>1</sup><sup>2</sup>

Pullman, Theodore N.; Lavender, A. R.; Aho, Impi; Rasmussen, Howard

doi:10.1210/endo-67-5-570

Cited by 89 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in those studies a possible contributory role by other major determinants of phosphorus excretion either was not evaluated or the studies were not designed in such a way as to penriit an adequate evaluation of their potential contribution. Factors known to increase urine phosphorus excretion which might become operative during acute hypercapnia include an increase in the release of parathyroid hormone (PTH)l (13)(14)(15) an enhancement of PTH effect on the kidney (16)(17)(18), and extracellular fluid (ECF) volume expansion (19)(20)(21). Furthermore, changes in either the blood or urine pH (22)(23)(24)(25)(26) or an increase in the blood bicarbonate concentration or renal reabsorption of bicarbonate (23,24,27,28) might also influence renal phosphate reabsorption.…”

Section: Introductionmentioning

confidence: 99%

Relationship between Phosphaturia and Acute Hypercapnia in the Rat

Webb¹,

Woodhall²,

Tisher³

et al. 1977

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Relationship between Phosphaturia and Acute Hypercapnia in the Rat

Webb¹,

Woodhall²,

Tisher³

et al. 1977

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…The mechanism of its phosphaturic effect is probably related to an inhibitory action on tubular phosphate reabsorption (17,18). An effect of PTH also upon urinary excretion of sodium has been periodically observed since 1935 (19) but, as many preparations of PTH have had variable effects on renal blood flow and GFR (20), the physiological mechanism of the observed rise in sodium excretion and its relationship to the phosphaturia is unclear.…”

Section: Introductionmentioning

confidence: 99%

Mode of action of parathyroid hormone and cyclic adenosine 3′,5′-monophosphate on renal tubular phosphate reabsorption in the dog

Agus¹,

Puschett²,

Senesky³

et al. 1971

J. Clin. Invest.

257

108

View full text Add to dashboard Cite

ABSTRA CT To evaluate the effects of parathyroid hormone and cyclic adenosine monophosphate on proximal tubular sodium and phosphate reabsorption, micropuncture studies were performed on dogs that received a highly purified preparation of parathyroid hormone (PTH), dibutyryl cyclic 3',5'-adenosine monophosphate (cyclic AMP), 5'-AMP, and saline. PTH resulted in a 30-40% inhibition of sodium and phosphate reabsorption in the proximal tubule unassociated with a rise in either total kidney or single nephron glomerular filtration rate (GFR). The bulk of the phosphate rejected proximally was excreted in the final urine while sodium excretion rose minimally despite the marked proximal inhibition, consistent with the presence of reabsorptive sites in the distal nephron for sodium but not phosphate. The infusion of dibutyryl cyclic AMP either systemically or directly into the renal artery inhibited proximal sodium and phosphate reabsorption in the absence of changes in either total kidney or single nephron GFR, resembling the effects of PTH quantitatively and qualitatively. In contrast, another adenine nucleotide, 5'-AMP, did not inhibit the reabsorption of either sodium or phosphate. These observations support the thesis that renal effects of PTH are mediated via stimulation of renal cortical adenyl cyclase. The infusion of a moderate saline load, 25 ml/kg, also produced a similar inhibition of proximal tubular fractional sodium and phosphate reabsorption with a marked phosphaturia but only minimal natriuresis. Thus, changes in An abstract of this work was published previously (1970. J. Clin. Invest. 49: 77a.).

show abstract

“…The primary actions of PTH occur in kidney and bone tissues. In kidney, PTH promotes renal tubular calcium resorption and synthesis of 1,25-dihydroxyvitamin D 3 but prevents resorption of phosphate (8). In bone, PTH increases osteoclastic bone resorption as part of calcium homeostasis, and the stimulatory effects of PTH on osteoblasts increase bone mass (9).…”

mentioning

confidence: 99%

Molecular recognition of parathyroid hormone by its G protein-coupled receptor

Pioszak

2008

Proc. Natl. Acad. Sci. U.S.A.

238

313

View full text Add to dashboard Cite

Parathyroid hormone (PTH) is central to calcium homeostasis and bone maintenance in vertebrates, and as such it has been used for treating osteoporosis. It acts primarily by binding to its receptor, PTH1R, a member of the class B G protein-coupled receptor (GPCR) family that also includes receptors for glucagon, calcitonin, and other therapeutically important peptide hormones. Despite considerable interest and much research, determining the structure of the receptor-hormone complex has been hindered by difficulties in purifying the receptor and obtaining diffraction-quality crystals. Here, we present a method for expression and purification of the extracellular domain (ECD) of human PTH1R engineered as a maltose-binding protein (MBP) fusion that readily crystallizes. The 1.95-Å structure of PTH bound to the MBP-PTH1R-ECD fusion reveals that PTH docks as an amphipathic helix into a central hydrophobic groove formed by a three-layer ␣-␤-␤␣ fold of the PTH1R ECD, resembling a hot dog in a bun. Conservation in the ECD scaffold and the helical structure of peptide hormones emphasizes this hot dog model as a general mechanism of hormone recognition common to class B GPCRs. Our findings reveal critical insights into PTH actions and provide a rational template for drug design that targets this hormone signaling pathway.calcitonin ͉ crystal structure ͉ glucagon ͉ maltose-binding protein ͉ osteoporosis

show abstract

DIRECT RENAL ACTION OF A PURIFIED PARATHYROID EXTRACT¹²

Cited by 89 publications

References 0 publications

Relationship between Phosphaturia and Acute Hypercapnia in the Rat

Relationship between Phosphaturia and Acute Hypercapnia in the Rat

Mode of action of parathyroid hormone and cyclic adenosine 3′,5′-monophosphate on renal tubular phosphate reabsorption in the dog

Molecular recognition of parathyroid hormone by its G protein-coupled receptor

Contact Info

Product

Resources

About

DIRECT RENAL ACTION OF A PURIFIED PARATHYROID EXTRACT12

Cited by 89 publications

References 0 publications

Relationship between Phosphaturia and Acute Hypercapnia in the Rat

Relationship between Phosphaturia and Acute Hypercapnia in the Rat

Mode of action of parathyroid hormone and cyclic adenosine 3′,5′-monophosphate on renal tubular phosphate reabsorption in the dog

Molecular recognition of parathyroid hormone by its G protein-coupled receptor

Contact Info

Product

Resources

About

DIRECT RENAL ACTION OF A PURIFIED PARATHYROID EXTRACT¹²