Direct Sensitivity Test of the MB/BacT System

Evaluation of indirect susceptibility testing of Mycobacterium tuberculosis to the first- and second-line, and alternative drugs by the newer MB/BacT system

“…Drugs and concentrations used -The concentrations of these drugs in the LJ (Canetti et al 1969) and the MB/ BacT followed the recommendations of Beer et al (1997b) and Barreto et al (2002) in the proportions indicated in Table I. Except for RIF, which was obtained from Pharmacia (Spa, Italy), all the drugs were obtained from Sigma Chemical Co. (St. Louis, Missouri, US).…”

Standardization of first and second-line antitubercular susceptibility testing using BacT Alert 3D system: a report from a tertiary care centre in India

“…AST was performed by the conventional proportion method using LJ medium and automation in the BacT Alert 3D system [6,9,10,11,12,13,14,15,16]. All the LJ subcultures of Mycobacteria were ≤3 weeks old and ≤36 hours, in the BacT/ Alert MP bottle.…”

“…The stock solutions were stored in small aliquots at -70°C. Working solution (1000µg/mL) was prepared on the day of the tests and added to the LJ medium and MB Bact medium to achieve the critical concentration (Table 1) [2,6,9,[11][12][13][14][15]. At least 3 critical concentration of each of the drugs were tested in the BacT Alert 3D system to determine the ideal concentration for the strains prevailing in the local environment Pfyffer et al 1999).…”

“…BacT Alert 3D Proportion Method [6,[9][10][11][12][13][14][15] The BacT/Alert MP bottle containing the growth of Mycobacterium tuberculosis (≤36 hours, subculture, then the growth was diluted 1:1 in sterile distilled water). This formed the direct growth control of approximately Mc Farland no.2 (DGC).…”

“…[6][7][8]. The radiometric and non-radiometric systems have been evaluated extensively for isolation and susceptibility to first line anti tubercular agents [9,10,11,12,13,14]. Very few studies have been conducted to evaluate the second line antitubercular drug susceptibility testing [2,14,15].…”