Direct sequencing and analysis of the genomes of newly emerging GII.17 norovirus strains in South China

Xue, L.; Cai, Weiping; Wu, Qingping; Zhang, Jumei; Guo, Weipeng

doi:10.1111/jam.13052

Cited by 12 publications

(9 citation statements)

References 23 publications

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“…In the past decades, NoV GII.4 was identified as the most predominant genotype involved in numerous epidemic outbreaks, for example, in 2002,2004,2006,2009 and 2012 [37]. Recently, a novel NoV GII.17 variant has emerged and is responsible for multiple disease outbreaks mainly in China and Japan [9,[11][12][13][14][15][16][17][18][19][20][21][22]. GII.17 appeared as a dominant strain replacing the GII.4 strain in these regions during 2014-2015 [23].…”

Section: Discussionmentioning

confidence: 99%

“…In the winter of 2014-2015, a rare NoVs genotype, GII.17, emerged in most of the East Asian regions including China (Guangdong [9][10][11][12][13], Jiangsu [14], Zhejiang [15], Hebei [16], Hong Kong [17], Taiwan [18], Beijing [19], and Shanghai [20], Japan [21] and South Korea [22]). Soon after, the novel GII.17 strain became the most predominant NoV strain, replacing the pandemic strain GII.4 Sydney 2012, responsible for the majority of gastroenteritis outbreaks in this region [23].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Norovirus GII.17: The Emergence and Global Prevalence of a Novel Variant

Yu¹,

Pan²,

Yan³

et al. 2018

Genotyping

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A rare norovirus (NoV) genotype GII.17 has recently emerged and rapidly became predominant in most East Asian countries in the winters of 2014-2015. In this study, we report the diversity of NoV GII.17 in detail; a total of 646 GII.17 sequences obtained during 1978-2015 were analyzed and subjected to meta-analysis. At least five major recombinant GII.17 clusters were identified. Each recombinant variant group appeared to have emerged following the time order: GII.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Norovirus GII.17: The Emergence and Global Prevalence of a Novel Variant

Yu¹,

Pan²,

Yan³

et al. 2018

Genotyping

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“…Due to the lack of a suitable in vitro validation system, genetic information of different NoV strains is necessary to identify their genetic diversity and evolution characteristics. In our previous studies, a “4 + 1 + 1” amplification strategy was used to obtain GII.4 and GII.17 genome sequences, which could be completed in one working day 25, 26 . The next-generation sequencing was employed for assembling of NoV genome recently 8, 27 .…”

Section: Discussionmentioning

confidence: 99%

Comparative phylogenetic analyses of recombinant noroviruses based on different protein-encoding regions show the recombination-associated evolution pattern

Xue

Dong

et al. 2017

Sci Rep

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Noroviruses are the major cause of acute gastroenteritis worldwide, and recombination is recognized as the important mechanism for its continuous emergence. In this study, for the common GII.P12 and GII.3 recombinants, phylogenetic relationships based on different proteins in three ORFs were comparatively analyzed, focusing on the influence of intergenic recombination. By using newly designed primers, genomes of two GII.P12/GII.3 Guangzhou recombinants were firstly amplified. Combined with other reported sequences of GII.P12_ORF1 (n = 20), GII.3_ORF2 (n = 131), GII.3_ORF3 (n = 36), all GII.P12 and GII.3 strains could be divided into 6, 8, and 7 clusters based on different ORFs, which showed an obvious recombination-associated and temporally sequential evolution pattern (with the exception of GII.P12/GII.13 recombinants). Based on multiple alignments, 126 informative sites were identified in three ORFs (44, 54, and 28), and four proteins (p48, p22, VP1, and VP2) were found under positive selection. Furthermore, by using homology modeling, predicted epitopes were mapped on the P proteins of seven GII.3 representative strains, without one (Epi: 353–361) specific to the GII.4 VA387 strain. In summary, via the genome analyses, phylogenetic relationships of GII.P12 and GII.3 recombinants based on the different proteins presented a special temporally sequential evolution process associated with their recombinant types.

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“…It attacked Beijing, Guang--dong, Hebei, Jiangsu, Zhejiang, Taiwan and Hongkong [20][21][22][23][24][25][26][27][28][29][30][31][32] . Besides, other rare prevalent genotypes were also confirmed, including GII.6 [33,34] and GIV.1 [35] .…”

Section: Gii4 Syndey As the Course Of Outbreaks Inmentioning

confidence: 99%

“…China [15,[20][21][22][23][24][25][26][27][28][29][30][31][32] . It attacked Beijing, Guang--dong, Hebei, Jiangsu, Zhejiang, Taiwan and Hongkong [20][21][22][23][24][25][26][27][28][29][30][31][32] .…”

Section: Gii4 Syndey As the Course Of Outbreaks Inmentioning

confidence: 99%

Norovirus, the Next Target for Vaccine Development in China

Du¹,

Guo²

2017

J. Appl. Virol.

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Enteric virus-induced disease, even death, greatly affects the life quality of children <5 years of age in China, and imposes a heavy sociometric burden on family and government. The infectious diarrhea diseases and hand-foot-mouth disease (HFMD) have been enrolled in the list of notifiable infectious diseases in China. Fortunately, the highly valued rotavirus-induced diarrhea and enterovirus 71-induced HFMD can be prevented by the commercial vaccines. However, the underrated norovirus vaccine is still in progress, even norovirus was considered as the leading cause of acute gastroenteritis in children <5 years of age in the world, partially due to the inability to cultivate norovirus. Besides, the broad genetic and serotypic diversity of norovirus also complicates selection of candidate vaccine strain(s). Therefore, the primary objective of this article is to review norovirus epidemics in China recur to the data published in recent five years.

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Direct sequencing and analysis of the genomes of newly emerging GII.17 norovirus strains in South China

Abstract: The newly developed method can be used as an important tool to collect genetic information of GII.17 noroviruses, and new obtained viral genomes in Guangzhou also provide reference data for norovirus research in future.

Cited by 12 publications

References 23 publications

Norovirus GII.17: The Emergence and Global Prevalence of a Novel Variant

Norovirus GII.17: The Emergence and Global Prevalence of a Novel Variant

Comparative phylogenetic analyses of recombinant noroviruses based on different protein-encoding regions show the recombination-associated evolution pattern

Norovirus, the Next Target for Vaccine Development in China

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