Direct sequencing of HPV DNA detected in gynaecologic outpatients in Rome, Italy

Verteramo, Rosita; Pierangeli, Alessandra; Calzolari, E; Patella, Alfredo; Recine, Nadia; Mancini, Emanuela; Marcone, Valentina; Masciangelo, Raffaele; Bucci, Mauro; Antonelli, Guido; Degener, Anna Marta

doi:10.1016/j.micinf.2006.07.001

Cited by 28 publications

(23 citation statements)

References 22 publications

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“…The prevalences reported in literature ranged from 20% to 50% of the infected subjects [Franco et al, 1999;Liaw et al, 2001;Nielsen et al, 2008]. The corresponding rate of 2.3% observed in women with normal cytology is consistent with the findings reported in previous Italian studies (1-2%) [Ronco et al, 2005;Verteramo et al, 2006]. To date it is not clear whether co-infection with multiple high-risk HPV types determines an increased risk of cervical neoplasia [Bosch et al, 2002;Van der Graaf et al, 2002].…”

Section: Discussionsupporting

confidence: 85%

“…Previous Italian studies conducted in selected areas of the country have shown rates of positivity ranging from 6.6% to 11% [De Francesco et al, 2005;Verteramo et al, 2006]. In a randomized controlled trial on more than 24,000 women aged 25-60 years from nine organized cervical screening programs, Ronco et al [2008] found an overall HPV positivity rate of 7.8%, with a rate of [Cuzick et al, 2006;de Sanjosè et al, 2007].…”

Section: Discussionmentioning

confidence: 95%

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Prevalence and type distribution of high‐risk human papillomavirus infection in women undergoing voluntary cervical cancer screening in Italy

Agarossi

Ferrazzi

Parazzini

et al. 2009

Journal of Medical Virology

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The aim of this survey was to assess the prevalence and distribution of oncogenic human papillomavirus (HPV) genotypes in women who underwent screening for cervical cancer in Italy. The correlation of genotypes with the cytological results was also evaluated. Cervical samples were collected from 9,947 self-referring women for cervical cancer screening. Participants were screened by liquid-based cytology and high-risk HPV testing using the Hybrid Capture 2 test. Positive samples were genotyped by PCR. Samples (1,474; 14.8%) were positive for high-risk HPV. The prevalence was 29.4% in the 15-19 years-group, decreasing progressively to 6.1% at 50-54 years of age and increasing to 12.2% in those aged over 65 years. HPV 16 was the genotype detected most frequently followed by HPV 31, HPV 18, HPV 56, and HPV 51. HPV 16 or 18 were present in 4% of women with normal cytology and both were detected contemporarily in only 14 women. Twenty-two percent of atypical squamous cells, 26% of low-grade and 56% of high-grade squamous intraepithelial lesions at cytology were positive for HPV 16 and/or 18. The prevalence of HPV infection in Italy is in agreement with that reported worldwide. HPV 16 was the prevalent genotype. The concomitant infection with HPV 16 and HPV 18 (vaccine targets) was found rarely. Apart from HPV 16 and 18, there was a substantial presence of HPV genotypes against which the vaccines available currently have shown cross-protection efficacy. The findings of this study may contribute to reliable predictions on the potential efficacy of an HPV vaccine in clinical practice.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 95%

Prevalence and type distribution of high‐risk human papillomavirus infection in women undergoing voluntary cervical cancer screening in Italy

Agarossi

Ferrazzi

Parazzini

et al. 2009

Journal of Medical Virology

View full text Add to dashboard Cite

show abstract

“…6 In fact, in another study, based on the same primers used in the present study, a similar prevalence of HPV types was observed. 21 Among HPV coinfections, association of HPV16 with other HPV types belonging to the same A9 species (i.e. HPV31) was observed.…”

Section: Discussionmentioning

confidence: 96%

Frequency and multiplicity of human papillomavirus infection in HIV-1 positive women in Italy

Garbuglia

Piselli

Lapa

et al. 2012

Journal of Clinical Virology

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“…HPV 16 pre va lans ora nı İngil tere' de %31.5 ve Çin' de %31.6 olup bi zim %33.3'lük bul gu mu za ben zer, 22,25 İtal ya' da ki ça lış ma da bil diri len %14.1 ve İspan ya' da ça lış ma po pü las yo nunda ki %20.7'lik oran lar ise dü şük tür. 28,29 Yi ne ça lış ma mız da %4.2 bu lu nan HPV 18 ora nı İtal ya' -dan bil di ri len %3.5'lik ora na ben zer dir. 29 Spe si fik HR HPV tip le ri nin pre va lan sı da ül ke ler ara sın da fark lı lık gös ter mek te dir.…”

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“…28,29 Yi ne ça lış ma mız da %4.2 bu lu nan HPV 18 ora nı İtal ya' -dan bil di ri len %3.5'lik ora na ben zer dir. 29 Spe si fik HR HPV tip le ri nin pre va lan sı da ül ke ler ara sın da fark lı lık gös ter mek te dir. İspan ya' da en yay gın HPV ti pi HPV 16 olup bu nu HPV 31 iz le mek te dir.…”

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The Prevalence of Human Papilloma Virus Infection Among Women who Admitted to Çukurova University Faculty of Medicine Hospital

Altun¹,

Yarkın²,

Vardar³

et al. 2011

Turkiye Klinikleri J Med Sci

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Ö ÖZ ZE ET T A Am ma aç ç: : Hu man papillomavirus (HPV) ser vi kal kan ser ile iliş ki si gös te ril miş ma jör et yo lo jik ajan dır. Ser vi kal kan ser tüm dün ya da ka dın lar ara sın da en yay gın ikin ci kan ser dir. Bu ça lış ma da Çu-ku ro va Üni ver si te si Tıp Fa kül te si Has ta ne si ne baş vu ran ka dın lar da hu man papillomavirus enfeksiyonu nun pre va lan sı nın araş tı rıl ma sı amaç lan mış tır. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Top lam 460 ser vi kal si to lo ji ör ne ği 20-68 yaş ara sı ka dın lar dan top lan mış tır. HPV DNA tes pi ti için MY09/11 ve GP5+/6+ pri mer le ri ile ya pı lan kon sen sus PCR (poly me ra se cha in re ac ti on) tes ti kul la nıl mış tır. HPV DNA pozi tif ör nek ler HPVpU-1M/pU-2R ve HPVpU-31B/pU-2R pri mer le ri ile sı ra sıy la yük sek risk li (HR; high risk) ve dü şük risk li (LR; low risk) tip ler ola rak iden ti fi ye edil miş tir. Ay rı ca HPV DNA po zitif bu lu nan ör nek ler ser vi kal kan ser de en yay gın gö rü len HR HPV tip le ri olan HPV 16, 18, 31 ve 45 için tip spe si fik PCR ile ge no tip len di ril miş tir. B Bu ul l g gu u l la ar r: : Top lam 460 ör nek ten 24 (%5.2)'ü HPV DNA için po zi tif bu lun muş tur. HPV DNA po zi tif 24 ka dın dan 14'ün de (%3) tek ve ya mul tip le enfeksiyon şek lin de HR HPV ti pi ve 10'un da (%2.2) ise tek ba şı na LR HPV po zi tif li ği tes pit edil miş -tir. HPV DNA po zi tif 30 yaş ve üs tü olan 24 ka dın da %33.3 ora nı ile en sık gö rü len HR HPV ti pi HPV tip 16 olup bu nu sı ra sıy la HPV 45 (%20.8), HPV 18 (%4.2) ve HPV 31 (%4.2) iz le miş tir. S So o n nu uç ç: : Çu ku ro va Üni ver si te si Tıp Fa kül te si Has ta ne sine baş vu ran ka dın lar da ge ni tal HPV enfeksiyon pre va lan sı %5.2 bu lun muş tur. HPV enfeksiyonu nun epi de mi yo lo jik araş tır ma la rı nın ser vi kal kan ser ta ra ma stra te ji le ri nin ge liş ti ril me si ve HPV aşı la rı nın uy gun lu ğu nun gös te ril me si için ge lecek te de sür dü rül me si öne ril mek te dir.A An na ah h t ta ar r K Ke e l li i m me e l le er r: : İnsan papillomavirusü 16; in san papillomavirusü 18; po li me raz zin cir re ak si yo nu; ute rin ser vi kal tü mör ler; pre va lans A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : Hu man pa pil lo ma virüs (HPV) is the ma jor eti o lo gic agent for cer vi cal cancer. Cer vi cal can cer is the se cond most pre va lent can cer among wo men in the world. In this study, we ai med to in ves ti ga te the pre va len ce of HPV in fec ti on among wo men ad mit ted to Hos pi tal of Çu -ku ro va Uni ver sity Fa culty of Me di ci ne. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : A to tal num ber of 460 cer vi cal smears we re ob ta i ned from wo men aged bet we en 20-68 ye ars. Con sen sus poly me ra se cha in re ac ti on (PCR) as say using MY09/11 and GP5 + /6 + pri mers we re used to de tect HPV DNA. HPV DNA po si ti ve samp les we re furt her stra ti fi ed as high risk (HR) and low risk (LR) by using HPVpU-1M/pU-2R and HPVpU-31B/pU-2R pri mers, res pec ti vely. HPV DNA po ...

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Direct sequencing of HPV DNA detected in gynaecologic outpatients in Rome, Italy

Cited by 28 publications

References 22 publications

Prevalence and type distribution of high‐risk human papillomavirus infection in women undergoing voluntary cervical cancer screening in Italy

Prevalence and type distribution of high‐risk human papillomavirus infection in women undergoing voluntary cervical cancer screening in Italy

Frequency and multiplicity of human papillomavirus infection in HIV-1 positive women in Italy

The Prevalence of Human Papilloma Virus Infection Among Women who Admitted to Çukurova University Faculty of Medicine Hospital

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