Direct shoot regeneration and genetic fidelity analysis in finger millet using ISSR markers

Babu, G. Atul; Vinoth, A.; Ravindhran, R.

doi:10.1007/s11240-017-1319-z

Cited by 31 publications

(9 citation statements)

References 43 publications

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“…At the molecular level, the most efficient are molecular markers based on DNA (Tiwari et al, 2013). Among the molecular markers, one of the most used is the Inter Simple Sequence Repeat (ISSR) analysis which allows detecting the variation that occurs in microsatellite regions scattered in the genome (Sharma et al, 2014;Akdemir et al, 2016;Martinez-Estrada et al, 2017;Babu et al, 2018;Rohela et al, 2020).…”

Section: Introduction and Objectivesmentioning

confidence: 99%

Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona officinalis

2021

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Cinchona officinalis is an important species from the Andean cloud forest that has a low regeneration rate in natural populations. In vitro regeneration of C. officinalis has been successfully established but somaclonal variation was not evaluated. The regeneration pathway and the number of subcultures on somaclonal variation were evaluated using six ISSR primers that amplified 58 loci of Inter Simple Sequence Repeats (ISSR). A dendrogram based on Jaccard´s genetic distance between the subcultures and the donor plant was produced. The results show that indirect shoot regeneration induces somaclonal variation, in the presence 2,4-Dichlorophenoxyacetic acid (2,4-D) in combination with kinetin and 6-Benzylaminopurine (BAP). In combination with 1-Naphthaleneacetic acid (NAA) or with Indole-3-butyric acid (IBA), BAP produces genetically stable explants. The highest proliferation rate was achieved using BAP and IBA. The present research study suggests avoiding the use of 2,4-D when C. officinalis is propagated for reintroduction and restoration projects.

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Section: Introduction and Objectivesmentioning

confidence: 99%

Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona officinalis

2021

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“…& Chalermglin [48], Abutilon indicum [59], Fritillaria dagana [60], Eleusine coracana (L.) Gaertn. [61], Smallanthus sonchifolius (Poepp. and Endl.)…”

Section: Discussionmentioning

confidence: 99%

In Vitro Regeneration and ISSR-Based Genetic Fidelity Analysis of Orthosiphon stamineus Benth

et al. 2019

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Orthosiphon stamineus has been widely used as traditional remedy for various illnesses and diseases, such as cardiovascular diseases and epileptic seizures. In this study, direct regeneration through nodal segment of this species was attempted using Kinetin (6-Furfurylaminopurine) and IAA (indole-3-acetic acid). Optimum regeneration media was identified as MS media supplemented with 2.0 mg L−1 Kin plus 0.5 mg L−1 IAA. This yielded the highest number of shoots (5.57 ± 0.42) and leaves (20.53 ± 1.91) per explant. Acclimatization of the resulting in vitro regenerants was successful in all potting mixtures tested. However, potting mixture PF (1:1:1 ratio of black soil/red soil/compost) was identified as the best medium for acclimatization of this species, as it yielded 100% survival percentage after 90 days of acclimatization. Ten in vitro regenerants of O. stamineus were randomly collected after the third subculture and subjected to genetic variation analysis using inter-simple sequence repeat (ISSR) markers. Out of 20 ISSR markers tested, 10 working primers were observed to produce satisfactory amplification of bands, with an average of 7.11 bands per primer. A total of 610 bands were produced by the 10 primers. The percentage of polymorphism was observed to be very low, yielding only 7.32% polymorphism among all samples. Jaccard dissimilarity analysis was also conducted and very low genetic distance (about 0.1) was found among the in vitro regenerants and between the regenerants with the mother plant, thus ascertaining the clonal nature of the plantlets produced in this study.

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“…Previous studies on finger millet have identified several inherent challenges associated with in vitro regeneration, such as the severe recalcitrant nature, polyploidy, and genotypic dependence, which singly or collectively frustrate the plant tissue culture work and, consecutively, the crop improvement systems through transgenesis (Dosad and Chawla, 2016). Plant regeneration in finger millet using different explants in different genotypes has been reported, such as epicotyl (Patil et al, 2009), shoot apical meristem (Babu et al, 2018;Ngetich et al, 2018), mature seeds (Bayer et al, 2014;Pande et al, 2015), and mature embryos (Satish et al, 2016). These protocols provide an opportunity to improve in vitro plant regeneration studies in finger millet, although optimization is required for each genotype.…”

Section: Transgenesis For Blast Resistancementioning

confidence: 99%

Breeding Strategies and Challenges in the Improvement of Blast Disease Resistance in Finger Millet. A Current Review

Mbinda

Masaki

2021

Front. Plant Sci.

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Climate change has significantly altered the biodiversity of crop pests and pathogens, posing a major challenge to sustainable crop production. At the same time, with the increasing global population, there is growing pressure on plant breeders to secure the projected food demand by improving the prevailing yield of major food crops. Finger millet is an important cereal crop in southern Asia and eastern Africa, with excellent nutraceutical properties, long storage period, and a unique ability to grow under arid and semi-arid environmental conditions. Finger millet blast disease caused by the filamentous ascomycetous fungus Magnaporthe oryzae is the most devastating disease affecting the growth and yield of this crop in all its growing regions. The frequent breakdown of blast resistance because of the susceptibility to rapidly evolving virulent genes of the pathogen causes yield instability in all finger millet-growing areas. The deployment of novel and efficient strategies that provide dynamic and durable resistance against many biotypes of the pathogen and across a wide range of agro-ecological zones guarantees future sustainable production of finger millet. Here, we analyze the breeding strategies currently being used for improving resistance to disease and discuss potential future directions toward the development of new blast-resistant finger millet varieties, providing a comprehensive understanding of promising concepts for finger millet breeding. The review also includes empirical examples of how advanced molecular tools have been used in breeding durably blast-resistant cultivars. The techniques highlighted are cost-effective high-throughput methods that strongly reduce the generation cycle and accelerate both breeding and research programs, providing an alternative to conventional breeding methods for rapid introgression of disease resistance genes into favorable, susceptible cultivars. New information and knowledge gathered here will undoubtedly offer new insights into sustainable finger millet disease control and efficient optimization of the crop’s productivity.

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Direct shoot regeneration and genetic fidelity analysis in finger millet using ISSR markers

Cited by 31 publications

References 43 publications

Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona officinalis

Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona officinalis

In Vitro Regeneration and ISSR-Based Genetic Fidelity Analysis of Orthosiphon stamineus Benth

Breeding Strategies and Challenges in the Improvement of Blast Disease Resistance in Finger Millet. A Current Review

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