Direct single-molecule detection of CoA-SH and ATP by the membrane proteins TMEM120A and TMEM120B

Zhao, Changjian; Chen, Mutian; Liu, Xiaofeng; Yuan, Weidan; Li, Kaiju; Wang, Yu; Chen, Chen; Zhang, Ming; Dong, Yuhan; Xiao, Yuling; Deng, Dong; Geng, Jia

doi:10.1039/d3nr05054h

Cited by 1 publication

(1 citation statement)

References 36 publications

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“…The regression equation estimated is given as I pa = −0.0579x + 6.7055 with a regression coefficient of R 2 = 0.9919. The prepared device's diagnostic properties were analyzed through a limit of detection (LOD) of 0.23 µM, and it was calculated by using LOD = 3 × (standard deviation of low concentration)/(slope of the calibration plot) [32][33][34] in the wide linear ranges of 2.05-629 µM, shown in Table S3. The results from this study demonstrate that the synthesized CePO 4 @f-CNF does not simply have a smaller detection limit, but also has a larger linear range for HXA sensing.…”

Section: Determination Of Calibration Plot and Limit Of Detectionmentioning

confidence: 99%

Hydrothermally Synthesized Cerium Phosphate with Functionalized Carbon Nanofiber Nanocomposite for Enhanced Electrochemical Detection of Hypoxanthine

Kasare,

Wang

2024

Chemosensors

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This work presents the detection of hypoxanthine (HXA), a purine derivative that is similar to nucleic acids who overconsumption can cause health issues, by using hydrothermally synthesized cerium phosphate (CePO4) followed by a sonochemical approach for CePO4 decorated with a functionalized carbon nanofiber (CePO4@f-CNF) nanocomposite. The formation of the nanocomposite was confirmed with X-ray powder diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive spectroscopy (EDS). A CePO4@f-CNF nanocomposite is used to modify a glassy carbon electrode (GCE) to analyze the electrochemical detection of HXA. Cyclic voltammetry (CV), Electrochemical impedance spectroscopy (EIS), and Differential pulse voltammetry (DPV) were used to examine the electrochemical properties of the composite. As a result, the modified electrode exhibits a larger active surface area (A = 1.39 cm2), a low limit of detection (LOD) at 0.23 µM, a wide linear range (2.05–629 µM), and significant sensitivity. Therefore, the CePO4@f-CNF nanocomposite was used to study the real-time detection in chicken and fish samples, and it depicted significant results.

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Section: Determination Of Calibration Plot and Limit Of Detectionmentioning

confidence: 99%

Hydrothermally Synthesized Cerium Phosphate with Functionalized Carbon Nanofiber Nanocomposite for Enhanced Electrochemical Detection of Hypoxanthine

Kasare,

Wang

2024

Chemosensors

View full text Add to dashboard Cite

show abstract

Direct single-molecule detection of CoA-SH and ATP by the membrane proteins TMEM120A and TMEM120B

Abstract: Membrane proteins are vital resources for developing biosensors. TMEM120A is a membrane protein associated with human pain transmission and lipid metabolism, and recent studies have demonstrated its ability to transport...

Cited by 1 publication

References 36 publications

Hydrothermally Synthesized Cerium Phosphate with Functionalized Carbon Nanofiber Nanocomposite for Enhanced Electrochemical Detection of Hypoxanthine

Hydrothermally Synthesized Cerium Phosphate with Functionalized Carbon Nanofiber Nanocomposite for Enhanced Electrochemical Detection of Hypoxanthine

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