Direct somatic embryogenesis and plant regeneration from leaf explants of Limonium sinensis (Girard) Kuntze

Dam, Anandamoy; Paul, Santanu; Bandyopadhyay, Tapas Kumar

doi:10.1016/j.scienta.2010.06.016

Cited by 28 publications

(20 citation statements)

References 21 publications

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“…The choice of PGRs determine both the ability of the explant to respond and the mode of the morphogenic reaction. Usually, somatic embryogenesis is induced in the presence of auxin alone or in combination with cytokinins (Gaj 2004;Dam et al 2010), but in a few systems, cytokinins alone were found to be effective in somatic embryogenesis induction and maturation (Nanda and Rout 2003;Carra et al 2006). Cytokinin activity depends on the property of two classes of compounds; the naturally occurring N 6 -substituted adenine derivatives (Shaw 1994) and the synthetic phenylurea derivatives (Shudo 1994).…”

Section: Discussionmentioning

confidence: 99%

An efficient regeneration system via direct and indirect somatic embryogenesis for the medicinal tree Murraya koenigii

Paul

Dam

Bhattacharyya

et al. 2010

Plant Cell Tiss Organ Cult

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A reproducible protocol for direct and indirect somatic embryogenesis was established in a small aromatic tree, Murraya koenigii. Embryogenic callus was obtained from 90% zygotic embryonic axis (ZE) and 70% cotyledon (COT) explants in Murashige and Skoog (MS) basal medium supplemented with 8.88 lM 6-benzyladenine (BA) and 2.675 lM a-naphthaleneacetic acid (NAA). Globular somatic embryos were induced and further matured from such embryogenic callus by subsequent culture on the same basal media containing thidiazuron (TDZ) (2.27-9.08 lM). The highest frequency of somatic embryos (14.58 ± 0.42) was recovered from ZE-derived callus after 6 weeks. The age and type of explant and concentration of TDZ played an important role in the development of somatic embryos. Explants excised from 60-day-old seed differentiated from 96.67% of ZE explants and 86.67% from COT explants when cultured on MS basal medium supplemented with 4.54 and 9.08 lM TDZ, respectively, after 4 weeks. The best result obtained for the average frequency of somatic embryos (11.28 ± 0.32) was from ZE explants, which was significantly higher than COT explants (7.34 ± 0.97). Most of the somatic embryos (above 95%), irrespective of their origin, germinated after 4 weeks in 1/2 MS basal media containing 2.32 lM kinetin (KN) and 1.07 lM NAA. Well-rooted plantlets were successfully acclimatized.Histological analysis and scanning electron micrographs confirmed the initiation, development, and germination of somatic embryos from both explants.

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Section: Discussionmentioning

confidence: 99%

An efficient regeneration system via direct and indirect somatic embryogenesis for the medicinal tree Murraya koenigii

Paul

Dam

Bhattacharyya

et al. 2010

Plant Cell Tiss Organ Cult

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“…however, induction of embryogenesis by cytokinins alone has been reported in a number of plant species (3,7,10). in this experiment, it was found that the frequency of indirect somatic embryogenesis was regulated by both cytokinins and auxin, while the cytokinins individually were associated with a high frequency of direct somatic embryogenesis.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Plant Regeneration Via Somatic Embryogenesis from Leaf Explants ofMuscari Armeniacum

Wang

FengMei

LiJun

et al. 2013

Biotechnology & Biotechnological Equipment

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“…This pretreatment triggers the morphogenetic competency of the explant, leading to the reception of the signals for embryo development as reported in other species (Franklin et al 2006;Dam et al 2010).…”

Section: Pretreatment Of Explantsmentioning

confidence: 72%

Direct and Indirect Somatic Embryogenesis in Mango Ginger (Curcuma amada Roxb.)

Shajahan

Raju

Thilip

et al. 2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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Somatic embryogenesis is a developmental restructuring of somatic cells in the embryogenic pathway and forms a basis for the concept of totipotency in plant cells. Understanding the process of transition from vegetative to embryogenic cells and factors involved in the somatic embryogenesis and subsequent plant development represents a challenge for plant tissue culture studies in any selected species. Although several reviews have been published dealing somatic embryogenesis in several plant species, there is no recent information on somatic embryogenesis in mango ginger (Curcuma amada). Two different types of somatic embryogenetic pathways are available in mango ginger. They are indirect somatic embryogenesis (IDSE) and direct somatic embryogenesis (DSE). Indirect somatic embryogenesis occurs in friable embryogenic callus from leaf sheath explants that undergo an extreme proliferation before the development of somatic embryos, whereas in the direct somatic embryogenesis, two-step system of culture was followed. Initially, leaf sheath explants were subjected to a pretreatment under dark condition before they were transferred to the subculture medium for embryo development. Histological and scanning microscopic studies showed that the formation of translucent globular embryogenic cell suspension is an important visible stage during early somatic embryogenesis through indirect method. In the direct pathway, upon pretreatment, epidermal and subepidermal cells of the leaf sheath explants formed globular and elongated structures. The presence of clear protoderm in the globular embryos and procambial structures are crucial visible stages.

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Direct somatic embryogenesis and plant regeneration from leaf explants of Limonium sinensis (Girard) Kuntze

Cited by 28 publications

References 21 publications

An efficient regeneration system via direct and indirect somatic embryogenesis for the medicinal tree Murraya koenigii

An efficient regeneration system via direct and indirect somatic embryogenesis for the medicinal tree Murraya koenigii

Plant Regeneration Via Somatic Embryogenesis from Leaf Explants ofMuscari Armeniacum

Direct and Indirect Somatic Embryogenesis in Mango Ginger (Curcuma amada Roxb.)

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