Direct somatic embryogenesis from explants obtained from<i>in vitro</i>germinated embryonic axes of<i>Camellia sinensis</i>(L.) O. Kuntze

Seran, H. Thayamini; Hirimburegama, K.; Gunasekare, M. T. K.

doi:10.1080/14620316.2006.11512154

Cited by 8 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GLS is other pathway for tissue culture propagation of tea beside organogenesis and somatic embryo; or can be called by "transition regeneration" between them. This regeneration was first reported by Seran et al (2006); called as small succulent leaves (SSL) but from unremoved embryo axis's growth points. This strucuture was also reported later in Camellia nitidissima (Lu et al, 2013) and then called as nodu-lar embryonic structures (NES).…”

Section: Materials and Tissue Culture Conditionsmentioning

confidence: 97%

Protein Profile of Tissue Culture of TRI2025 Tea Clone

et al. 2019

View full text Add to dashboard Cite

Tea is well known as favourite healthy drink for almost all people over the world. Tea propagation using conventional and modern ways are now developing rapidly. However, information regarding the protein profile of tissue culture of tea plant has not been revealed yet. This study aimed to determine the difference of protein profile of tea's tissue culture using SDS-PAGE. This study was conducted using embryonic axes of TRI2025 tea clone cultured on MS media supplemented with 2,4-D for inducing somatic embryogenesis and globular-like structure (GLS) regeneration, and MS media supplemented with BAP for inducing shoot via organogenesis. The results revealed that proteins in the size of 37.69; 54.89; 60.77; 71.35; 87.34; and 92.99 KDa might be involved at somatic embryogenesis, and about 38.69 KDa, 69.27 KDa, and 55.76 KDa respectively for GLS, initiation of shoot, and initiation of GLS derived leaf. Predicted key protein for leaf initiation both directly or through GLS was about 31-33 KDa, while for callusing were about 27.56 and 52.73 KDa, and constitutive protein was about 22.75 KDa. This study provides the first report of protein profile of tea's tissue culture. The information obtained can be beneficial as a marker for explant for somatic embryogenesis, GLS, or organogenesis pathway and as a scientific information for further biotechnology development.

show abstract

Section: Materials and Tissue Culture Conditionsmentioning

confidence: 97%

Protein Profile of Tissue Culture of TRI2025 Tea Clone

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Initial attempts were focused on establishing aseptic in vitro cultures, optimization of explants type, nutrient composition of culture media and further multiplication (Mukhopadhyay et al 2015). Different explant types were used for micropropagation such as nodal segments (Agarwal et al 1992), shoot tips (Banerjee and Agarwal 1990), zygotic embryos (Iddagoda et al 1988;Ranaweera et al 2013;Seran et al 2006), embryogenic axes (Seran et al 2006), epidermal layers of stem segments (Kato 1985), axillary buds (Nakamura 1990) and leaves (Sarwar 1985). Different basal media formulations such as , White's medium (White 1963), Woody Plant Medium (WPM) (Lloyd and McCown 1980) and Schenk and Hildebrandt (1972), supplemented with various concentrations of growth regulators like BAP, Kinetin, NAA, GA3, IBA, Zeatin and TDZ (Das et al 2012b) for multiple shoot proliferation.…”

Section: Micropropagationmentioning

confidence: 99%

Advances in Plant Breeding Strategies: Nut and Beverage Crops

2019

View full text Add to dashboard Cite

show abstract

“…One alternative choice of its vegetative propagation is through tissue culture, both somatic embryogenesis or in vitro organogenesis. There have been many reports that provided information about the success of tea propagation, with different culture conditions, such as differences in plant growth regulators (PGRs), incubation time, and other tissue culture conditions (Seran et al 2006;Kaviani 2013;Gonbad et al 2014;Eskundari et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…One crucial point is the root-apical axis development that only occurs at explants induced through somatic embryogenesis (Bassuner et al 2006). In tea, there were many reports about somatic embryogenesis and in vitro organogenesis using different clones (Gunasekare & Evans 2000;Sandal et al 2005;Seran et al 2006;Kaviani 2013;Gonbad et al 2014), and TRI2025 tea clone has been reported for source clone of various explants; through both techniques (Akula & Dodd 1998;Seran et al 2006;Eskundari et al 2018). Besides specific clone requirements, successful of somatic embryogenesis and in vitro organogenesis also require specific PGRs to promote the explant's growth and development.…”

Section: Introductionmentioning

confidence: 99%

“…Besides specific clone requirements, successful of somatic embryogenesis and in vitro organogenesis also require specific PGRs to promote the explant's growth and development. Several studies in vitro related to somatic embryogenesis and in vitro organogenesis of tea plants were reported using PGR(s), such as auxin and cytokinin (Mondal et al 1998;Gunasekare & Evans 2000;Sandal et al 2005;Seran et al 2006;Kaviani 2013;Gonbad et al 2014).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture

Eskundari

Taryono

Indradewa

et al. 2021

J.Tropical Biodiversity Biotechnology

View full text Add to dashboard Cite

Tissue culture is an alternative choice of plant propagation either through somatic embryogenesis or in vitro organogenesis techniques. TRI2025 tea clone has been cultured successfully, however, the scientific information related to morphology, histology, and protein profile at an early event of culturing time has not been reported yet. This study aimed to determine the differences between those pathways, in the context of morphology, histology, and protein profile. The explants were the embryo axis of TRI2025 tea clone cultured on two different induction mediums; somatic embryogenesis and in vitro organogenesis induction medium. The results showed that most of the explants cultured on A medium developed to be a globular-like structure at 11-day after culture (DAC), while all explants cultured on B medium showed the initiation stage of in vitro organogenesis. Histological analysis showed meristem reconstruction at shoot apical meristem (SAM) and root apical meristem (RAM) at 11-DAC at explants cultured on B medium, while explants cultured on A medium showed callusing at 21-DAC. Protein profile analysis using SDS-PAGE showed protein bands of 54 and 81 KDa that only appeared at explants cultured on A medium start from 14-DAC, and those two protein bands thought to be a differentiator at the early stages of the two tissue culture techniques. Thus, these parameters can be used as early detection for plant tissue culture, especially in tea.

show abstract

Direct somatic embryogenesis from explants obtained fromin vitrogerminated embryonic axes ofCamellia sinensis(L.) O. Kuntze

Cited by 8 publications

References 0 publications

Protein Profile of Tissue Culture of TRI2025 Tea Clone

Protein Profile of Tissue Culture of TRI2025 Tea Clone

Advances in Plant Breeding Strategies: Nut and Beverage Crops

Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture

Contact Info

Product

Resources

About