Direct somatic embryogenesis from leaf and petiole explants of Spathiphyllum ‘Supreme’ and analysis of regenerants using flow cytometry

Zhao, Jietang; Chen, Jin; Liu, Juanxu; Feixiong, Liao; Henny, Richard J.; Chen, Jianjun

doi:10.1007/s11240-012-0146-5

Cited by 23 publications

(5 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Flow cytometry allows for rapid and accurate estimation of the nuclear DNA content in thousands of cells and has been widely used for rapid screening of the ploidy levels of regenerated plants like Centaurea ultreiae (Mallón et al 2010), Vitis vinifera (Prado et al 2010) or Spathiphyllum (Zhao et al 2012). Similarly, the analyses of the chromosome numbers and their structural aberrations by means of cytogenetic methods have been successfully employed for detecting the somaclonal variation in plants regenerated in vitro (Do et al 1999;MolnarLang et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Chromosome variations in regenerants of Arabidopsis thaliana derived from 2- and 6-week-old callus detected using flow cytometry and FISH analyses

Orzechowska

Stępień

Kamińska

et al. 2012

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Shoot organogenesis was induced from 2-and 6-week-old callus derived from the leaves of Arabidopsis thaliana ecotype Columbia (2n = 10). Regenerated plants were evaluated for chromosomal variations by means of flow cytometry and fluorescent in situ hybridization (FISH). Flow cytometric measurements revealed the occurrence of diploid, tetraploid, and octoploid plants among the regenerants of 2-week-old calli, whereas only diploid and tetraploid plants were regenerated from the 6-week-old calli. Chromosome counting showed that plants developed from the 2-week-old calli exhibited mixoploidy and a high frequency of aneuploid cells. These plants were infertile and displayed altered morphology. FISH with 5S and 25S rDNA probes allowed to detect some structural chromosomal rearrangements in regenerated plants. Along with cells which exhibited correct localisation of rDNA loci, also cells bearing chromosomal translocations, deletions or duplications were found. The type of structural aberrations varied between diploid and tetraploid regenerants.

show abstract

Section: Introductionmentioning

confidence: 99%

Chromosome variations in regenerants of Arabidopsis thaliana derived from 2- and 6-week-old callus detected using flow cytometry and FISH analyses

Orzechowska

Stępień

Kamińska

et al. 2012

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…However, direct somatic embryogenesis of cultured plant tissue may be an acceptable approach for the generation of genetically stable regenerated plants (Vasil et al 1988;Pedroso and Pais 1995;Zhang et al 2006). Moreover, somatic embryogenesis also reduces the time required for plant propagation which may reduce secondary changes occurring during the process of dedifferentiation (Denchev et al 1991;Zhao et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Direct somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Anthurium andraeanum Linden cv. Fantasia

Bhattacharya

Dam

Karmakar

et al. 2016

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

We present here a method for the in vitro propagation of Anthurium andraeanum cv. Fantasia through direct somatic embryogenesis. The embryos were directly formed at the cut end of most of the leaf explants when cultured on MS basal medium supplemented with N 6 -benzyladenine (BA) plus α-naphthalene acetic acid (NAA). MS basal media supplemented with BA (0.27 μM) and NAA (2.68 μM) induced maximum number of somatic embryos per leaf explant (15.33 ± 1.45) after 28 d of continuous culture. The highest numbers of embryos (12.33 ± 0.88) were matured into plantlets in MS basal medium augmented with 0.27 μM BA and 58.4 mM sucrose. Histology showed the presence of scutellum, coleoptile, welldeveloped root, and shoot initials at different stages of somatic embryo (SE) development directly on leaf explants. About 85% of the plantlets were successfully acclimatized, and of these, 80.3% plants survived after secondary hardening under greenhouse conditions. The embryo-derived plants successfully flowered. The presence of monomorphic DNA bands in random amplified polymorphic DNA (RAPD) marker analysis confirmed the genetic homogeneity of direct somatic embryo-derived plantlets in respect to their mother plant.

show abstract

“…Thus, immature embryos have been the most frequently used explants in somatic embryogenesis [24]. However, many studies have already been reported that plant regeneration is possible through somatic embryogenesis from leaf explants of species such as Phalaenopsis [25], peace lily [26], Citrullus colocynthis [27], Capsicum baccatum [28], Tolumnia species [29], Scaevola sericea [30], and Euryodendron excelsum [31]. In this study, embryogenic cells were successfully induced from the leaf explants of D. genkwa.…”

Section: Discussionmentioning

confidence: 94%

Efficient Plant Regeneration System from Leaf Explant Cultures of Daphne genkwa via Somatic Embryogenesis

Woo

Shin

et al. 2023

Plants

View full text Add to dashboard Cite

This study aimed to establish an efficient plant regeneration system from leaf-derived embryogenic structure cultures of Daphne genkwa. To induce embryogenic structures, fully expanded leaf explants of D. genkwa were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.1, 0.5, 1, 2, and 5 mg·L−1 2,4-dichlorophenoxyacetic acid (2,4-D), respectively. After 8 weeks of incubation, the highest frequency of embryogenic structure formation reached 100% when the leaf explants were cultivated on MS medium supplemented with 0.1 to 1 mg·L−1 2,4-D. At higher concentrations of 2,4-D (over 2 mg·L−1 2,4-D), the frequency of embryogenic structure formation significantly declined. Similar to 2,4-D, indole butyric acid (IBA) and α-naphthaleneacetic acid (NAA) treatments were also able to form embryogenic structures. However, the frequency of embryogenic structure formation was lower than that of 2,4-D. In particular, the yellow embryonic structure (YES) and white embryonic structure (WES) were simultaneously developed from the leaf explants of D. genkwa on culture medium containing 2,4-D, IBA, and NAA, respectively. Embryogenic calluses (ECs) were formed from the YES after subsequent rounds of subculture on MS medium supplemented with 1 mg·L−1 2,4-D. To regenerate whole plants, the embryogenic callus (EC) and the two embryogenic structures (YES and WES) were transferred onto MS medium supplemented with 0.1 mg·L−1 6-benzyl aminopurine (BA). The YES had the highest plant regeneration potential via somatic embryo and shoot development compared to the EC and WES. To our knowledge, this is the first successful report of a plant regeneration system via the somatic embryogenesis of D. genkwa. Thus, the embryogenic structures and plant regeneration system of D. genkwa could be applied to mass proliferation and genetic modification for pharmaceutical metabolite production in D. genkwa.

show abstract

Direct somatic embryogenesis from leaf and petiole explants of Spathiphyllum ‘Supreme’ and analysis of regenerants using flow cytometry

Cited by 23 publications

References 42 publications

Chromosome variations in regenerants of Arabidopsis thaliana derived from 2- and 6-week-old callus detected using flow cytometry and FISH analyses

Chromosome variations in regenerants of Arabidopsis thaliana derived from 2- and 6-week-old callus detected using flow cytometry and FISH analyses

Direct somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Anthurium andraeanum Linden cv. Fantasia

Efficient Plant Regeneration System from Leaf Explant Cultures of Daphne genkwa via Somatic Embryogenesis

Contact Info

Product

Resources

About