Direct spectrophotometric determination of quercetin in the presence of ascorbic acid

Pejić, Nataša; Kuntić, Vesna; Vujić, Zorica; Mićić, Svetlana

doi:10.1016/j.farmac.2003.07.013

Cited by 51 publications

(23 citation statements)

References 9 publications

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“…The spectrofluorimetric method exhibits the same advantages over the spectrophotometric methods suggested for the determination of quercetin in bulk drug and pharmaceutical formulations as well [5,18,19].…”

Section: T a B L Ementioning

confidence: 81%

“…Several methods were proposed in the literature to determine quercetin in the samples of apple and tomato juice and fruits, wines, teas, serums and pharmaceutical preparations. These include HPLC [13][14][15], HPTLC [16] LC-MS [17], spectroscopic methods [5,18,19], adsorptive stripping voltammetry [20], electrochemical analysis [21] and fluorimetric methods [22]. , CAS number 6151-25-3, Fluka AG, Buchs, Germany), acetonitrile (J.T.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms

Pavun

Đurđević

Jelikić-Stankov

et al. 2014

Maced. J. Chem. Chem. Eng.

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A simple, accurate and precise method based on the fluorescence properties of the aluminum(III)-quercetin complex, for the determination of quercetin, has been developed and validated. The complex has strong emission at pH 3.30,  em = 480 nm, with  ex = 420 nm. The linearity range of quercetin determination was 1.5-60.5 ng ml -1 with LOD 0.09 ng ml -1 and LOQ 0.27 ng ml -1. Recovery values in the range of 99.9-100.2% indicate a good accuracy of the method. The established method was applied for the determination of quercetin in capsules, with a recovery value of 98.3%, standard deviation of 0.22% and coefficient of variation of 0.09%.The reliability of the method was checked by the newly developed RP-HPLC/UV method for capsules with the direct determination of quercetin after separation. The good agreement between the two methods indicates the applicability of the proposed spectrofluorimetric method for quercetin determination in pharmaceutical dosage forms, with high reproducibility, and enables the direct and simple determination without its prior extraction from samples.The proposed spectrofluorimetric method has much better sensitivity and LOD and LOQ values that are about 1000 times lower than data reported in the literature.Keywords: quercetin; flavonoids; spectrofluorimetric determination; RP-HPLC; capsules СПЕКТРОФЛУОРОМЕТРИСКО ОПРЕДЕЛУВАЊЕ НА КВЕРЦЕТИН ВО ФАРМАЦЕВТСКИ ДОЗИРАНИ ФОРМИРазвиен и валидиран е едноставен, точен и прецизен метод за определување на кверцетин заснован на флуоресцентните својства на комплексот кверцетин-алуминиум(III). Комплексот покажува силна емисија при pH 3,30,  em = 480 nm и  ex = 420 nm. Линеарниот опсег за определување на кверцетин е 1,5-60,5 ng ml -1 со LOD 0,09 ng ml -1 и LOQ 0,27 ng ml -1. Вредностите на аналитичкиот принос од 99,9-100,2% покажуваат добра точност на методот. Воспоставениот метод е применет за определување на кверцетин во капсули со вредност на аналитичкиот принос од 98.3%, стандардна девијација од 0,22% и коефициент на варијација од 0,09%.Веродостојноста на методот е проверена со новововедениот RP-HPLC/UV метод за капсули со директно определување на кверцетин по негова сепарација. Доброто согласување на двата метода укажува на применливост на предложениот спектрофлуорометриски метод за опреде-лување на кверцетин во фармацевтски дозирани форми со висока репродуцибилност и овозможува директно и едноставно определување без претходна екстракција од примероците.Предложениот спектрофлуорометриски метод има многу подобра осетливост и вредностите LOD и LOQ се за околу 1000 пати пониски од оние дадени во литературата.

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Section: T a B L Ementioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms

Pavun

Đurđević

Jelikić-Stankov

et al. 2014

Maced. J. Chem. Chem. Eng.

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“…Several methods were proposed in the literature to determine QRC in the samples of apple and tomato juice and fruits, wines, teas, serums and pharmaceutical preparations. These include HPLC [8][9][10], HPTLC [11] LC-MS [12], spectroscopic methods [13,14], adsorptive stripping voltammetry [15], electro-chemical analysis [16] and fluorimetric methods [17]. The majority of methods required some pre-treatment of real samples, such as solid phase extraction [18] and molecularly imprinted polymers (MIPs).…”

Section: Introductionmentioning

confidence: 99%

Normal Flow and Stopped Flow Injection Spectrophotometric Determination of Quercetin Dihydrate Dietary Supplements

2017

IJSR

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Simple and sensitive normal flow injection(nFI) and stopped-FI spectrophotometric methods for the determination of quercetin dehydrate (QRC) in pure form were proposed, The methods were based on diazotization and coupling reaction between QRC and diazotized metoclopramide in alkaline medium to form orange-water soluble dye. The dye is stable and has a maximum absorbance at 438nm. A calibration graph shows that a Beers law is obeyed over the concentration range of 1-60µg.ml -1 and 0.8-50µg.ml -1 for (QRC) with a detection limit is 0.4166 µg.ml

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“…One of the major problems in the determination of rutin by electrochemical methodology is interference. As flavonoids, rutin can often be found together with ascorbic acid (AA) 9) and may hydrolyze to quercetin in pharmaceutical preparations if not well stored. Rutin has the similar oxidation potential on the bare electrodes with AA or quercetin.…”

mentioning

confidence: 99%

Determination of Rutin on the Poly(p-aminobenzene sulfonic acid) Modified Glassy Carbon Electrode

et al. 2010

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475Rutin (3Ј,4Ј,5,7-tetrahydroxyflavone-3b-D-rutinoside) is one of the most bioactive flavonoids, also known as vitamin P and was thought to be an activating factor for Vitamin C. 1) Rutin has been shown to act as a scavenger of various oxidizing species, superoxide anion (O 2 Ϫ ), hydroxyl radical and peroxyl radicals.2) As a result of this, its several pharmacological activities have been widely exploited including antibacterial, antiinflammatory, antitumor, antiallergic, antiviral and antiprotozoal properties. 3,4) Hence, the determination of rutin is of considerable interest. Capillary electrophoresis, 5) HPLC,6) flow injection analysis, 7) spectrophotometry 8) and electrochemical techniques have been used to determine rutin.Electrochemical methods for the determination of rutin are more sensitive, less expensive and less time-consuming than the other methods. One of the major problems in the determination of rutin by electrochemical methodology is interference. As flavonoids, rutin can often be found together with ascorbic acid (AA) 9) and may hydrolyze to quercetin in pharmaceutical preparations if not well stored. Rutin has the similar oxidation potential on the bare electrodes with AA or quercetin. Electrodes modified with b-cyclodextrin incorporated carbon nanotube, 10) gilo peroxidase, 11) CeO 2 nanoparticle, 12) ionic liquid 13) have been used for the determination of rutin in the presense of AA or quercetin. However, these modified electrodes are tedious to prepare and costly or lack of stability. It is necessary to detect rutin in convenient, sensitive and high selective methods till date.Conducting polymers have been widely used as electrode modifiers for their good stability, reproducibility, more active sites and homogeneity in the past two decades. Polymers based on aniline and aniline derivatives have been of particular interest due to their many desirable features such as facile preparation, environmental stability, high conductivity, etc. Recently, poly(p-aminobenzene sulfonic acid) modified electrodes have been reported for the electrochemical study of several compounds, including dopamine, 14) uric acid, In this work, a poly( p-aminobenzene sulfonic acid) modified glassy carbon electrode (PABSA/GC) was fabricated by electropolymerization and the electrochemical behavior of rutin was investigated at it. It was found PABSA/GC showed excellent electrocatalytic activity for the oxidation of rutin. A method for the determination of rutin with simple, stable, sensitive and selective characteristics was developed. The proposed method was further used for the determination of rutin tablet samples with satisfactory results. ExperimentalReagents Rutin (C 27 H 30 O 16 · 3H 2 O, Mϭ664.56) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) and its stock solution (1.0ϫ10 Ϫ3 M) was prepared with ethanol. p-Aminobenzene sulfonic acid (p-ABSA) was of analytical-reagent grade from Tianjin BASF Chemical Co. The supporting electrolyte was a 0.1 M phosphate buffer saline (PBS) (pH 4.0). O...

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Direct spectrophotometric determination of quercetin in the presence of ascorbic acid

Cited by 51 publications

References 9 publications

Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms

Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms

Normal Flow and Stopped Flow Injection Spectrophotometric Determination of Quercetin Dihydrate Dietary Supplements

Determination of Rutin on the Poly(p-aminobenzene sulfonic acid) Modified Glassy Carbon Electrode

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