Direct Spectrophotometric Determination of Serum and Urinary Oxalate with Oxalate Oxidase

Kohlbecker, G.; Butz, M.

doi:10.1515/cclm.1981.19.11.1103

Cited by 9 publications

(7 citation statements)

References 11 publications

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“…Predialysis oxalic acid and total homocysteine levels also were determined. Plasma oxalic acid was determined by the oxalate oxidase enzymatic method (12) by Cerba Laboratories (Ile‐de‐France, France). For these determinations, the whole blood was immediately centrifuged and frozen at −70°C.…”

Section: Methodsmentioning

confidence: 99%

Water‐Soluble Vitamin Levels in Patients Undergoing High‐Flux Hemodialysis and Receiving Long‐Term Oral Postdialysis Vitamin Supplementation

Descombes¹,

Boulat²,

Perriard

et al. 2000

Artificial Organs

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The prescription of multivitamin supplements for dialysis patients is routine practice, but the doses prescribed differ greatly from one dialysis center to another. Few data are available concerning long-term vitamin supplementation and its effects on patients either on high-flux hemodialysis or receiving postdialysis supplementation. For several years, we have systematically prescribed to our patients an oral postdialysis multivitamin supplement containing thiamine hydrochloride 100 mg, riboflavin 20 mg, pyridoxine hydrochloride 50 mg, folic acid 6 mg, and ascorbic acid 500 mg. The aim of this study was to perform a cross-sectional long-term evaluation of the vitamin levels in patients who received this vitamin supplement for at least 12 months. We also were interested in investigating the plasma oxalic acid and total homocysteine levels associated with the long-term prescription of these vitamin supplements. Thirty-three patients on high-flux dialysis were studied. Vitamin levels and/or vitamin-dependent enzymatic activities were within the normal range (N) in all patients. The mean results (+/-SD) were plasma ascorbic acid 13.6 +/- 6.4 mg/L (N > 4), plasma folate 14.1 +/- 1.1 microg/L (N > 3), for vitamin B1, alpha-ETK 1.02 +/- 0.02 (N < 1.18) and ETKo 100 +/- 13 U/L (N > 70), for vitamin B2, alpha-EGR 1.00 +/- 0.07 (N < 1.52) and EGRo 1282 +/- 213 U/L (N > 672), and for vitamin B6, alpha-EGOT 1.34 +/- 0.10 (N < 1.8) and EGOTo 380 +/- 84 U/L (N > 228). Plasma oxalic acid was higher than normal in all patients (mean = 61 +/- 15 micromol/L, N < 33). However, all patients had oxalic acid levels within the range reported in the literature for patients not taking extra ascorbic acid. Mean total homocysteine was 24 +/- 8 micromol/L with only 4 patients (12%) having normal levels (N < 15). In conclusion, the postdialysis supplement given provides adequate vitamin levels in almost all patients in the long term. Postdialysis prescription allows an optimal compliance with the treatment, is well accepted by the patients, and is cost-effective.

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Section: Methodsmentioning

confidence: 99%

Water‐Soluble Vitamin Levels in Patients Undergoing High‐Flux Hemodialysis and Receiving Long‐Term Oral Postdialysis Vitamin Supplementation

Descombes¹,

Boulat²,

Perriard

et al. 2000

Artificial Organs

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“…Analytical Procedures. Oxalate was analyzed by a new enzymatic method recently described (17,18). Ascorbate was determined with a modified method according to Zannoni et al (25).…”

Section: Methodsmentioning

confidence: 99%

Dietary Influence on Serum and Urinary Oxalate in Healthy Subjects and Oxalate Stone Formers

Butz

Hoffmann

Kohlbecker³

1980

Urol Int

Self Cite

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With a new enzymatic method, the dietary influence of oxalate, glycine, protein, and ascorbic acid on serum and urinary oxalate has been examined. Healthy and oxalate stone-forming subjects were compared. Two doses of sodium oxalate (130 and 400 mg daily) were administered. The high dose induced significant hyperoxaluria. No changes of serum oxalate were seen. Neither glycine (4.5 g daily) nor protein (50 g daily, 50% animal protein) had any effect on serum or urinary oxalate. Urinary oxalate excretion did not increase upon ingestion of large amounts of ascorbic acid (1–6 g daily), but serum oxalate levels were significantly elevated. The value of severe dietary restrictions concerning the compounds examined here seems to be questionable, as a significant increase of urinary oxalate excretion is lacking.

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“…Oxalate oxidase converts oxalate and oxygen to carbon dioxide and hydrogen peroxide (Chiriboga 1966), and such activity has been detected in various plants (Pundir and Pundir 1993). The enzyme has attracted attention as a reagent for the specific measurement of oxalate in urine (Kohlbecker and Butz 1981;Pundir 1991), although the reaction mechanism and its possible metal or other cofactor dependence is currently unknown (Sugiura et al 1979;Lane 1994).…”

Section: Introductionmentioning

confidence: 99%

Modeling based on the structure of vicilins predicts a histidine cluster in the active site of oxalate oxidase

1998

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It is known that germin, which is a marker of the onset of growth in germinating wheat, is an oxalate oxidase, and also that germins possess sequence similarity with legumin and vicilin seed storage proteins. These two pieces of information have been combined in order to generate a 3D model of germin based on the structure of vicilin and to examine the model with regard to a potential oxalate oxidase active site. A cluster of three histidine residues has been located within the conserved beta-barrel structure. While there is a relatively low level of overall sequence similarity between the model and the vicilin structures, the conservation of amino acids important in maintaining the scaffold of the beta-barrel lends confidence to the juxtaposition of the histidine residues. The cluster is similar structurally to those found in copper amine oxidase and other proteins, leading to the suggestion that it defines a metal-binding location within the oxalate oxidase active site. It is also proposed that the structural elements involved in intermolecular interactions in vicilins may play a role in oligomer formation in germin/oxalate oxidase.

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Direct Spectrophotometric Determination of Serum and Urinary Oxalate with Oxalate Oxidase

Cited by 9 publications

References 11 publications

Water‐Soluble Vitamin Levels in Patients Undergoing High‐Flux Hemodialysis and Receiving Long‐Term Oral Postdialysis Vitamin Supplementation

Water‐Soluble Vitamin Levels in Patients Undergoing High‐Flux Hemodialysis and Receiving Long‐Term Oral Postdialysis Vitamin Supplementation

Dietary Influence on Serum and Urinary Oxalate in Healthy Subjects and Oxalate Stone Formers

Modeling based on the structure of vicilins predicts a histidine cluster in the active site of oxalate oxidase

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