Direct stereological estimation of three-dimensional connectivity in rat vertebrae: Effect of estrogen, etidronate and risedronate following ovariectomy

Boyce, Rogely; Wronski, Thomas J.; Ebert, D.C.; Stevens, M.L.; Paddock, Carol L.; Youngs, T.A.; Gundersen, H.J.G.

doi:10.1016/8756-3282(94)00031-t

Cited by 37 publications

(22 citation statements)

References 23 publications

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“…The results indicate that ovariectomy decreased lumbar vertebra bone strength by altering bone mass, bone microarchitecture, and intrinsic bone tissue quality, which is consistent with previously published findings observed in this model [16][17][18][24][25][26][27][28][29][30]. In contrast, in the conditions of this study, there were no reductions in the mechanical strength of the midshaft femur in OVX control rats 52 weeks after ovariectomy when compared to SHAM control rats (mean maximum load±SD=182.64±17.85 N for OVX vs. 171.41±17.47 N for SHAM).…”

Section: Discussionsupporting

confidence: 82%

Strontium ranelate improves bone strength in ovariectomized rat by positively influencing bone resistance determinants

Bain

Jerome²,

Shen³

et al. 2008

Osteoporos Int

126

111

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Section: Discussionsupporting

confidence: 82%

Strontium ranelate improves bone strength in ovariectomized rat by positively influencing bone resistance determinants

Bain

Jerome²,

Shen³

et al. 2008

Osteoporos Int

126

111

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“…In addition, the higher dose of Cenestin appeared to maintain trabecular bone strength in the presence of estrogen deficiency. Many currently available anti-resorptive agents approved by the US FDA have been found to maintain trabecular bone mass and bone strength in the rat model for estrogen deficiency bone loss [3,[25][26][27][28][29][30]. However, most of these studies used standard histomorphometry, which provides only two-dimensional information about trabecular bone mass and structure and bone turnover.…”

Section: Discussionmentioning

confidence: 99%

“…Other investigators have similarly reported that treatment of acutely estrogen-deficient rats with other antiresorptive agents can maintain trabecular bone mass, structure, and strength [3,11,12,[25][26][27][28][29][30][31]. Lane et al [3], using three-dimensional high-resolution microscopy to visualize trabecular bone, reported that estrogen treatment, initiated 13 days following ovariectomy after 20% of trabecular bone volume and trabecular connections had been lost, restored trabecular bone volume by increasing trabecular thickness [3].…”

Section: Discussionmentioning

confidence: 99%

The Effects of Synthetic Conjugated Estrogens, A (Cenestin) on Trabecular Bone Structure and Strength in the Ovariectomized Rat Model

Lane

Kumer

Majumdar

et al. 2002

Osteoporosis International

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This study evaluated the effect of Cenestin, a synthetic conjugated estrogens product, on the maintenance of trabecular bone microarchitecture, bone strength, and of bone turnover in the ovariectomized (ovx) rat model. Two doses of Cenestin were chosen in an attempt to approximate the equivalent human oral doses of 0.3 mg and 0.625 mg. Forty-nine 6-month-old retired female breeder Sprague-Dawley rats were randomly assigned to one of four groups: (1) sham-operated + vehicle; (2) ovx + vehicle; (3) ovx + day 1 post-ovariectomy Cenestin (8.12 mg/kg); (4) ovx + day 1 post-ovariectomy Cenestin (16.24 mg/kg) for 8 weeks. Trabecular structure of the right proximal tibia of each rat was imaged noninvasively by microCT. A compression test to induce a tibial plateau fracture was performed to determine the mechanical properties of the proximal tibia. Urine was collected on days 0, 14, 28, 42 and 56 and serum on days 0, 28 and 56 to assess biochemical markers of bone turnover including deoxypyridinoline crosslinks and osteocalcin. Both biochemical markers of bone turnover were analyzed by ELISA. Trabecular bone mass, structure, and connectivity density in the Cenestin-treated groups did not differ statistically ( p>0.05) from those of the sham-operated + vehicle-treated rats, but all were significantly higher ( p<0.05) than in the ovx + vehicle-treated rats. Structure Model Index, a measure of trabecular plate morphometry, in Cenestin-treated rats maintained a more equal mix of plate- and rod-like structures similar to the sham group, whereas the ovx group had predominantly rod-like trabeculae. Fracture load in the Cenestin (16.24 mg/kg) treated group was 31% ( p<0.01) higher than in the sham + vehicle-treated group and 61% ( p<0.05) higher than in the ovx + vehicle-treated group. Both the sham-operated + vehicle-treated and Cenestin-treated groups showed significantly lower urinary deoxypyridinoline crosslink excretion at all timepoints and serum osteocalcin at day 56 compared with the ovx + vehicle-treated group. In summary, Cenestin maintained trabecular bone microarchitecture and strength in an ovariectomized rat model of estrogen deficiency.

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“…I t seems that connectivity may be a n important parameter for monitoring structural changes under pathological conditions, such as osteoporosis. Investigation based on stereological technique lias shown that tlie reduction in tlie connectivity of vertebral cancellous bone is a longterm consequence of ovariectomy in tlie rat [2].…”

Section: Discussionmentioning

confidence: 99%

Age‐related variations in the microstructure of human tibial cancellous bone

Ding

Odgaard

Linde

et al. 2002

Journal Orthopaedic Research

168

129

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A thorough understanding of the microstructure of cancellous bone is crucial for diagnosis, prophylaxis, and treatment of agerelated skeletal diseases. Until now, little has been known about age-related variations in the microstructure of peripheral cancellous bone. This study quantified age-related changes in the tliree-dimensioiial (3D) microstructure of huiiian tibial cancellous bone. One hundred and sixty cylindricul cnncellous bone specimens were produced from 40 normal proxinial tibiae froiii 40 donors, aged 16-85 years. These speciiiiciis were micro-computed tomography (inicro-CT) scanned, and microstructural propei-tics werc dctcrinincd. The specimens were h e n tested in compression to obtain Young's iiioduliis.The degree of anisotropy, mean marrow space volume, and bone surface-to-volume ratio increased significantly with age. Bone volunie fraction, mean trabecular volume, and bone surface density decreased significantly with age. Connectivity did not have a general relationship with age. Bone voltiine fraction together with anisotropy best predicted Young's modiilus. Age-related changes in the microstructural properties had the same trends for both iiiedial and lateral condyles of the tibia. The observed increase of anisotropy and constant connectivity suggest a bone remodeling mechanism that may reorient trabecular volume orientation in aging tibial cancellous bone. The aging trabeculae align more strongly to the priinary direction -parallel to the tibial longitudinal loading axis. 0 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved. liitroductioiiTlie incidence of age-related skeletal diseases, such as osteoartlirosis [ 171 and osteoporosis [ 19,301 has increased in the past two decades, as a result of an increase in tlie elderly population and a change in lifestyle. These diseases are major health care problems in t e r m of socio-econoinic costs. A tlioi-ougli understanding of agerelated changes in tlie microstructure of caticellous bone is crucial for diagnosis, prophylaxis, a i d treatmcnt of these age-related skeletal diseases, and for the design, fixation and durability of joint prostheses.Previous investigations 011 the microstructure of cancellous bone have demonslrated significant agere la ted changes i ii ca ~icellous bone micros t r tic t u ra 1 parameters, such as bone volume fraction and bone [I ,29,3 1,341. but one study includes femoral cancellous bone [20]. However, tlie method used in this latter study [10] has been recognized to bc subject to serious limitations [9,24]. Therefore, no gencrnl coticlusions for age-related variations in the microstructure of human peripheral cancellous bone are available.

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Direct stereological estimation of three-dimensional connectivity in rat vertebrae: Effect of estrogen, etidronate and risedronate following ovariectomy

Cited by 37 publications

References 23 publications

Strontium ranelate improves bone strength in ovariectomized rat by positively influencing bone resistance determinants

Strontium ranelate improves bone strength in ovariectomized rat by positively influencing bone resistance determinants

The Effects of Synthetic Conjugated Estrogens, A (Cenestin) on Trabecular Bone Structure and Strength in the Ovariectomized Rat Model

Age‐related variations in the microstructure of human tibial cancellous bone

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