Direct testing of the biasing effect of manipulations of endolymphatic pressure on cochlear mechanical function

Abstract

Abstract. The history of cochlear mechanical investigations has been carried out in two largely separate sets of endeavours; those interested in auditory processing in animal models and those interested in the origin of adverse vestibular symptoms in humans. In respect of the first, mechanical vibratory data is considered pathological and not representative of pristine behaviour if it departs from the reigning model of sharp tuning and high hearing sensitivity. Conversely, when the description of the pathological behaviour is the focus, fluid movements responsible for hearing loss and vestibular symptoms dominate. Yet both extensive sets of data possess a common factor now being reconsidered for its potential to shed light on the mechanisms in general. The common factor is a mechanical bias -the departure of cochlear epithelial membranes from their usual resting position. In both cases the bias modulates hearing sensitivity and distorts tuning characteristics. Indeed several early sets of guinea pig mechanical data were dismissed as "pathological" when in hindsight, the primary effect influencing the data was not loss of outer hair cell function per se, but a mechanical bias unknowingly introduced in process of making the measurement. Such biases in the displacement of the basilar membrane from its position are common, and may be caused by low-frequency sounds (topically including infrasound) or by variations in fluid volume in the chambers particularly applying the case of endolymphatic hydrops. Most biases are quantified in terms of visualisation of fluid volume change, electric potential changes and otoacoustic emissions. Notably many previous studies have also searched for raised pressures with negative results. Yet these repeated findings are contrary to the widespread notion that, at least when homeostasis is lost, it is a rise in endolymphatic pressure which is responsible for membrane rupture and Meniere's attacks. This current investigation in Mongolian gerbils is aimed at quantifying hydrostatic pressures in cochlear chambers by direct measurement using a null-flow micropipette pressure measurement system, while simultaneously quantifying electric potentials and distortion products to provide indirect measures of displacement bias and hair cell integrity. We now suspect that during any experiment obtaining of good pressure seals is critical. Secondary penetrations, such as occur in neural recordings, are contra-indicated. When we address the issue of seals we see raised pressures in response to manipulations known to disturb homeostasis, viz. diuretics and hypoxia.

A role for the otoliths in the mechanics of cochlear homeostasis?

“…A new computational model is here presented for the function of an otolith organ, following interest in homeostatic regulation mechanisms centred on the endolymphatic system [5,13]. Any regulatory mechanism for pressure should reasonably possess a pressure sensor, so where, within the membranous labyrinth is one located?…”

“…This duplication is puzzling considering cochlear low-frequency reception is generally less vulnerable than high. Unexplained is how pressures within the labyrinth are regulated [5]. A hypothesis is developed that the saccule's low-frequency pressure sensitivity may represent another function entirely, connected with the mechanics of cochlear homeostasis.…”