Direct toxicity assessment of copper (II) ions to activated sludge process using a p-benzoquinone-mediated amperometric biosensor

Wang, Xuejiang; Zhang, Jing; Bu, Yunjie; Yan, Xiangbo; Chen, Jie; Huang, Jiayu; Zhao, Jianfu

doi:10.1016/j.snb.2014.11.089

Cited by 12 publications

(4 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the approaches to the development of a sensitive express system is the use of a stable microbial association that is sensitive to various kinds of toxicants (heavy metals, organic substances). With the luminescent type of toxicity detection, it is quite difficult to vary the biomaterial, so special attention is paid to the electrochemical biosensors [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. Unlike luminescent biosensors, the amperometric type of detection applies to a significant number of single microorganisms and forms of bioreceptors based on consortia.…”

Section: Introductionmentioning

confidence: 99%

“…Unlike luminescent biosensors, the amperometric type of detection applies to a significant number of single microorganisms and forms of bioreceptors based on consortia. Quite often, the main biomaterials used to assess toxicity are E. coli bacteria [ 19 , 23 , 24 ], S. cerevisiae yeast [ 19 , 25 ], and activated sludge [ 21 , 26 ]. Thus, the possibility of creating a whole-cell electrochemical biosensor based on the mediator p-benzoquinone for detecting heavy metal ions present in wastewater was investigated [ 17 ]; the biomaterial included an association of microbial strains such as E. coli , B. subtilis , and S. cerevisiae , which allowed for increasing the sensitivity of registration compared with the use of single E. coli microorganisms and the mediator [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mediator Microbial Biosensor Analyzers for Rapid Determination of Surface Water Toxicity

Kharkova

Arlyapov

Medvedeva

et al. 2022

Sensors

View full text Add to dashboard Cite

Microbial mediator biosensors for surface water toxicity determination make it possible to carry out an early assessment of the environmental object’s quality without time-consuming standard procedures based on standard test-organisms, and provide broad opportunities for receptor element modifying depending on the required operational parameters analyzer. Four microorganisms with broad substrate specificity and nine electron acceptors were used to form a receptor system for toxicity assessment. Ferrocene was the most effective mediator according to its high rate constant of interaction with the microorganisms (0.33 ± 0.01 dm3/(g × s) for yeast Saccharomyces cerevisiae). Biosensors were tested on samples containing four heavy metal ions (Cu2+, Zn2+, Pb2+, Cd2+), two phenols (phenol and p-nitrophenol), and three natural water samples. The «ferrocene- Escherichia coli» and «ferrocene-Paracoccus yeei, E. coli association» systems showed good operational stability with a relative standard deviation of 6.9 and 7.3% (14 measurements) and a reproducibility of 7 and 5.2% using copper (II) ions as a reference toxicant. Biosensor analysis with these systems was shown to highly correlate with the results of the standard method using Chlorella algae as a test object. Developed biosensors allow for a valuation of the polluted natural water’s impact on the ecosystem via an assessment of the influence on bacteria and yeast in the receptor system. The systems could be used in toxicological monitoring of natural waters.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mediator Microbial Biosensor Analyzers for Rapid Determination of Surface Water Toxicity

Kharkova

Arlyapov

Medvedeva

et al. 2022

Sensors

View full text Add to dashboard Cite

show abstract

“…It is reported that the spill of cyanide and other illegal chemical dumping can cause the shutdown of WWTPs and consequently release contaminants to the surrounding environment [14]. Toxicity assessment methods require development for monitoring the change of influent toxicity and guiding the rapid response strategies to protect WWTPs from the shock load [15].…”

Section: Introductionmentioning

confidence: 99%

Monitoring the Activated Sludge Activities Affected by Industrial Toxins via an Early-Warning System Based on the Relative Oxygen Uptake Rate (ROUR) Index

Chen²,

Zou

et al. 2019

Applied Sciences

View full text Add to dashboard Cite

Shock load from industrial wastewater is known to harm the microbial activities of the activated sludge in wastewater treatment plants (WWTPs) and disturb their performance. This study developed a system monitoring the activated sludge activities based on the relative oxygen uptake rate (ROUR) and explored the influential factors with wastewater and the activated sludge samples collected from a typical WWTP in the Taihu Lake of southern Jiangsu province, China. The ROUR was affected by the concentration of toxic substances, mixed liquid suspended solids (MLSS), hydraulic retention time (HRT) and pH. Higher toxin contents significantly decreased the ROUR and the EC50 value of Zn2+, Ni2+, Cr(VI), Cu2+, and Cd2+ was 13.40, 15.54, 97.56, 12.01, and 14.65 mg/L, respectively. The ROUR declined with the increasing HRT and MLSS above 2000 mg/L had buffering capacities for the impacts of toxic substances to some extent. The ROUR remained stable within a broad range pH (6–10), covering most of the operational pH in WWTPs and behaving as an appropriate indicator for monitoring the shock load. A toxicity model assessing and predicting the ROUR was developed and fitted well with experimental data. Coupling the ROUR monitoring system and toxicity model, an online early-warning system was assembled and successfully used for predicting the toxicity of different potential toxic metals. This study provides a new universal toxicity model and an online early-warning system for monitoring the shock load from industrial wastewater, which is useful for improving the performance of WWTPs.

show abstract

“…18 The use of BQ-mediated recognition elements in toxicity biosensor systems has become common. Wang et al 19 successfully developed a simple, rapid, and reliable amperometric-mediated biosensor, which adopted BQ as a mediator, to determine the toxicity of heavy metal pollutants to the activated sludge environment. Zhi et al 16 proved that BQ was more efficient for biotoxicity assay than K 3 [Fe(CN) 6 ] in a simple microbial BQmediated biosensor fabricated by immobilizing E. coli on a glassy carbon electrode with gelatin solutions.…”

mentioning

confidence: 99%

Colorimetric and Electrochemical Dual-Signal Method for Water Toxicity Detection Based on Escherichia coli and p-Benzoquinone

Sun

et al. 2021

ACS Sens.

View full text Add to dashboard Cite

The development of simple and rapid toxicity detection methods has important practical significance. In this work, a dual-signal method with colorimetric and electrochemical properties for water toxicity detection was proposed for the first time based on a rapid color reaction between Escherichia coli (E. coli) and p-benzoquinone (BQ). Here, E. coli was used as a biocatalyst and BQ was used as a mediator. An IC50 value of 0.75 mg L–1 for Cu2+ was obtained using a two-step electrochemical detection method. Strikingly, toxicity could also be estimated visually by the naked eye, and the minimum detection limit was 3.2 mg L–1 for Cu2+. The dual-signal toxicity detection method extends the function of BQ, and the result is more reliable than the traditional single-signal method. This simple and rapid toxicity detection method shows certain application prospects.

show abstract

Direct toxicity assessment of copper (II) ions to activated sludge process using a p-benzoquinone-mediated amperometric biosensor

Cited by 12 publications

References 28 publications

Mediator Microbial Biosensor Analyzers for Rapid Determination of Surface Water Toxicity

Mediator Microbial Biosensor Analyzers for Rapid Determination of Surface Water Toxicity

Monitoring the Activated Sludge Activities Affected by Industrial Toxins via an Early-Warning System Based on the Relative Oxygen Uptake Rate (ROUR) Index

Colorimetric and Electrochemical Dual-Signal Method for Water Toxicity Detection Based on Escherichia coli and p-Benzoquinone

Contact Info

Product

Resources

About