Direct visualization of carbon nanotube degradation in primary cells by photothermal imaging

Russier, Julie; Oudjedi, Laura; Piponnier, Martin; Bussy, Cyrill; Prato, Maurizio; Kostarelos, Kostas; Lounis, Brahim; Bianco, Alberto; Cognet, Laurent

doi:10.1039/c6nr09795b

Cited by 29 publications

(19 citation statements)

References 35 publications

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“…In particular, the measurement of the integrated intensity ratio between the D and G bands (A D /A G ) is a way to semi-quantify the degree of structural defects in the nanotube sample (Table 1). 33,34,36,45,55,56 Fig. 1b displays representative Raman spectra of p-MWCNT, 2 and 9 h pre-oxidized MWCNT samples showing the characteristic D and G bands at ca.…”

Section: Resultsmentioning

confidence: 99%

“…Their lysosomes, apart from displaying a pH of 4.5, contain a large variety of enzymes (acid-hydrolases and peroxidases) that are able to gradually digest CNTs. [27][28][29][30][31][32][33] Macrophages, 32 microglia, [33][34][35][36] neutrophils 37,38 and eosinophils 31 have all been confirmed to degrade CNTs in culture after different previous surface treatments. Paradoxically, phagocytes are more susceptible than other cells to CNT-toxicity, at least in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Biodegradable multi-walled carbon nanotubes trigger anti-tumoral effects

et al. 2018

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Carbon nanotubes are of huge biotechnological interest because they can penetrate most biological barriers and, inside cells, can biomimetically interact with the cytoskeletal filaments, triggering anti-proliferative and cytotoxic effects in highly dividing cells. Unfortunately, their intrinsic properties and bio-persistence represent a putative hazard that relapses their application as therapies against cancer. Here we investigate mild oxidation treatments to improve the intracellular enzymatic digestion of MWCNTs, but preserving their morphology, responsible for their intrinsic cytotoxic properties. Cell imaging techniques and confocal Raman spectroscopic signature analysis revealed that cultured macrophages can degrade bundles of oxidized MWCNTs (o-MWCNTs) in a few days. The isolation of nanotubes from these phagocytes 96 hours after exposure confirmed a significant reduction of approximately 30% in the total length of these filaments compared to the control o-MWCNTs extracted from the cell culture medium, or the intracellular pristine MWCNTs. More interestingly, in vivo single intratumoral injections of o-MWCNTs triggered ca. 30% solid melanoma tumour growth-inhibitory effects while displaying significant signs of biodegradation at the tumoral/peri-tumoral tissues a week after the therapy has had the effect. These results support the potential use of o-MWCNTs as antitumoral agents and reveal interesting clues of how to enhance the efficient clearance of in vivo carbon nanotubes.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biodegradable multi-walled carbon nanotubes trigger anti-tumoral effects

et al. 2018

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“…The understanding of the pathways leading to the removal of nanomaterials from the organism is of major importance for their final application and is a required issue for the clearance of use in clinical trials. Nanocomposites, firstly considered as structurally persistent, were shown to be degraded by oxidative enzymes, among others, peroxidases [54][55][56][57]. It was reported that single layer and few layers graphene is degraded by human MPO, which is released by neutrophils [58].…”

Section: Discussionmentioning

confidence: 99%

Graphene Oxide Nanosheets for Localized Hyperthermia—Physicochemical Characterization, Biocompatibility, and Induction of Tumor Cell Death

Podolska

Barras

Frey

et al. 2020

Cells

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Background: The main goals of cancer treatment are not only to eradicate the tumor itself but also to elicit a specific immune response that overcomes the resistance of tumor cells against chemo- and radiotherapies. Hyperthermia was demonstrated to chemo- and radio-sensitize cancerous cells. Many reports have confirmed the immunostimulatory effect of such multi-modal routines. Methods: We evaluated the interaction of graphene oxide (GO) nanosheets; its derivatives reduced GO and PEGylated rGO, with components of peripheral blood and evaluated its thermal conductivity to induce cell death by localized hyperthermia. Results: We confirmed the sterility and biocompatibility of the graphene nanomaterials and demonstrated that hyperthermia applied alone or in the combination with radiotherapy induced much more cell death in tumor cells than irradiation alone. Cell death was confirmed by the release of lactate dehydrogenase from dead and dying tumor cells. Conclusion: Biocompatible GO and its derivatives can be successfully used in graphene-induced hyperthermia to elicit tumor cell death.

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“…peroxidases) are able to catalyse the degradation of graphene oxide or carbon nanotubes in test tubes, in vitro and in vivo. [45][46][47][48] In addition, our very recent review carefully evaluated the role of the material properties including the number of layers, the lateral dimension and the C/O ratio in the degradation ability of each specific GBM (Fig. 4).…”

Section: Biodegradabilitymentioning

confidence: 99%