2021
DOI: 10.1186/s13068-021-02073-y
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Directed evolution and secretory expression of xylose isomerase for improved utilisation of xylose in Saccharomyces cerevisiae

Abstract: Background Xylose contained in lignocellulosic biomass is an attractive carbon substrate for economically viable conversion to bioethanol. Extensive research has been conducted on xylose fermentation using recombinant Saccharomyces cerevisiae expressing xylose isomerase (XI) and xylose reductase/xylitol dehydrogenase (XR/XDH) pathways along with the introduction of a xylose transporter and amplification of the downstream pathway. However, the low utilization of xylose in the presence of glucose… Show more

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Cited by 12 publications
(5 citation statements)
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“…We have recently discussed the various engineering approaches used to rebuild these pathways for the generation of xylose-fermenting S. cerevisiae strain and the different strategies applied to improve xylose utilization, such as eliminating cofactor specificities, development of pentose transporters, and native metabolic pathway engineering in detail elsewhere (Yusuf and Gaur, 2017). More developments on the subject can also be found in the recently published articles (Bae et al, 2021;Cunha et al, 2020b;Hou et al, 2017). Instead, in this review, we have focussed on studies that have engineered S. cerevisiae for utilizing xylose and other non-cellulosic sugars to produce bioethanol in a mixed sugar state for CBP applications.…”
Section: Engineering Yeast To Produce Ethanol From Hemicellulosic Fee...mentioning
confidence: 99%
“…We have recently discussed the various engineering approaches used to rebuild these pathways for the generation of xylose-fermenting S. cerevisiae strain and the different strategies applied to improve xylose utilization, such as eliminating cofactor specificities, development of pentose transporters, and native metabolic pathway engineering in detail elsewhere (Yusuf and Gaur, 2017). More developments on the subject can also be found in the recently published articles (Bae et al, 2021;Cunha et al, 2020b;Hou et al, 2017). Instead, in this review, we have focussed on studies that have engineered S. cerevisiae for utilizing xylose and other non-cellulosic sugars to produce bioethanol in a mixed sugar state for CBP applications.…”
Section: Engineering Yeast To Produce Ethanol From Hemicellulosic Fee...mentioning
confidence: 99%
“…Was improved by directed evolution and then introduced into S. cerevisiae for secretory expression. In fermentation with xylose or glucose/xylose, the xylose consumption and ethanol production of the engineered S. cerevisiae were improved markedly ( Bae et al, 2021 ).…”
Section: Synthesis Of Value-added Bioproducts From Xylose Through Met...mentioning
confidence: 99%
“…In particular, endocellulase, exocellulase, and β-glucosidase are secreted by yeast for the utilization of cellulosic biomass [ 15 , 16 ]. In addition, engineered yeast secretes xylose isomerase and xylanase to utilize hemicellulose [ 17 , 18 ]. However, relatively little research has been conducted on laccase or peroxidase secretion in yeast.…”
Section: Introductionmentioning
confidence: 99%