Directed migration of cancer cells guided by the graded texture of the underlying matrix

Park, Jin Seok; Kim, Deok Ho; Kim, Hong Nam; Wang, Chiaochun Joanne; Kwak, Moon Kyu; Hur, Eun Mi; Suh, Kahp Y.; An, Steven S.; Levchenko, Andre

doi:10.1038/nmat4586

Cited by 173 publications

(174 citation statements)

References 47 publications

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“…Various components of the extracellular matrix (ECM), such as laminin have also been implicated in modulation of cell migration (Friedl and Wolf, 2009, Petrie et al, 2009, Wirtz et al, 2011, Anton et al, 1999, Porcionatto, 2006). Directional cell migration can be guided by a variety of mechanical cues presented by ECM structures ranging in size from nanometers (nm) to microns (µm) (Kim et al, 2009a, Kim et al, 2012, Park et al, 2016). Therefore, a challenge is to develop an experimental platform that will model the mechano-chemical cellular milieu, yet remain simple and accessible to allow practical, high-throughput use.…”

Section: Introductionmentioning

confidence: 99%

Migration Phenotype of Brain-Cancer Cells Predicts Patient Outcomes

et al. 2016

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SUMMARY Glioblastoma multiforme is a heterogeneous and infiltrative cancer with dismal prognosis. Studying the migratory behavior of tumor-derived cell populations can be informative but places a high premium on the precision of in vitro methods and their relevance of in vivo conditions. In particular, the analysis of 2D cell migration may not reflect invasion into 3D extracellular matrices in vivo. Here we describe a method that allows time-resolved studies of primary cell migration with single-cell resolution on a fibrillar surface that closely mimics in vivo 3D migration. We used this platform to screen 14 patient-derived glioblastoma samples. We observed that the migratory phenotype of a subset of cells in response to Platelet-derived growth factor was highly predictive of tumor location and recurrence in the clinic. Therefore, migratory phenotypic classifiers analyzed at the single-cell level in a patient-specific way can provide high diagnostic and prognostic value for invasive cancers.

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Section: Introductionmentioning

confidence: 99%

Migration Phenotype of Brain-Cancer Cells Predicts Patient Outcomes

et al. 2016

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“…Interestingly, the asymmetric micro‐ and nanostructures also cause cell adhesion asymmetry when they are in contact with cells, causing cell‐cytoskeletal polarization and indirectly inducing the generation of different cellular behaviours . Previous surfaces designed for generating anisotropic cell migration have usually been constructed with gradient factors including gradient arrays or gradient Young's modulus substrates . The driving forces of cell migration triggered by this type of microstructure are mainly derived from the gradient effect factor of the surface.…”

Section: Figurementioning

confidence: 99%

Adhesion Anisotropy Substrate with Janus Micropillar Arrays Guides Cell Polarized Migration and Division Cycle

et al. 2019

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Cell migration is a ubiquitous and important cell behaviour. Construction of an interface that can guide and induce cell migration is of significance for studying cell behaviour and carrying out the transplantation of biological materials. Herein, we prepare a micropillar array by precise modification of two different functional groups on each micropillar unit to obtain a “Janus” structure with anisotropic cell‐adhesion properties. This anisotropic structure was capable of influencing the focal adhesion pathway of melanoma cells as shown by transcriptome sequencing and bioinformatics analysis. This the anisotropic substrate can considerably affect the migration ability of highly invasive melanoma cells. The anisotropic structure with Janus micropillar arrays could also affect the cell division cycle. The experiments showed that the anisotropic microstructures regulate cell migration and are thus of use in the research of cell migration without drug stimulation.

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“…[123] Recently, Levchenko and colleagues described the guided migration of invasive and non-invasive melanoma cells according to the gradient in matrix nanotopograpy. [124] They showed that invasive (non-invasive) cells migrated towards sparser (denser) areas and concluded that the topotactic response depends on both the density and structure of the ECM, and the stiffness of the cell itself.…”

Section: Spatial Patterns Of Topographymentioning

confidence: 99%

Mechanotransduction and Growth Factor Signalling to Engineer Cellular Microenvironments

Cipitria

Salmerón-Sánchez

2017

Adv Healthcare Materials

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Engineering cellular microenvironments involves biochemical factors, the extracellular matrix (ECM) and the interaction with neighbouring cells. This progress report provides a critical overview of key studies that incorporate growth factor (GF) signalling and mechanotransduction into the design of advanced microenvironments. Materials systems have been developed for surface‐bound presentation of GFs, either covalently tethered or sequestered through physico‐chemical affinity to the matrix, as an alternative to soluble GFs. Furthermore, some materials contain both GF and integrin binding regions and thereby enable synergistic signalling between the two. Mechanotransduction refers to the ability of the cells to sense physical properties of the ECM and to transduce them into biochemical signals. Various aspects of the physics of the ECM, i.e. stiffness, geometry and ligand spacing, as well as time‐dependent properties, such as matrix stiffening, degradability, viscoelasticity, surface mobility as well as spatial patterns and gradients of physical cues are discussed. To conclude, various examples illustrate the potential for cooperative signalling of growth factors and the physical properties of the microenvironment for potential applications in regenerative medicine, cancer research and drug testing.

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Directed migration of cancer cells guided by the graded texture of the underlying matrix

Cited by 173 publications

References 47 publications

Migration Phenotype of Brain-Cancer Cells Predicts Patient Outcomes

Migration Phenotype of Brain-Cancer Cells Predicts Patient Outcomes

Adhesion Anisotropy Substrate with Janus Micropillar Arrays Guides Cell Polarized Migration and Division Cycle

Mechanotransduction and Growth Factor Signalling to Engineer Cellular Microenvironments

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