Directing cell function and fate via micropatterning: Role of cell patterning size, shape, and interconnectivity

Poudel, Ishwari; Menter, Daniel E.; Lim, Jung Yul

doi:10.1007/s13534-012-0045-z

Cited by 12 publications

(8 citation statements)

References 50 publications

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“…Finally, a broad spectrum of in vitro cell confinement models have been proposed with the aim to reproduce cell constraints by controlling cell shape, area and spreading ( Poudel et al, 2012 ). Cell body confinement on micropatterned surfaces has been shown to control the commitment of stem cells to a specific lineage.…”

Section: Biological Responses To Cellular Mechanosensingmentioning

confidence: 99%

Cellular Mechanotransduction: From Tension to Function

et al. 2018

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Living cells are constantly exposed to mechanical stimuli arising from the surrounding extracellular matrix (ECM) or from neighboring cells. The intracellular molecular processes through which such physical cues are transformed into a biological response are collectively dubbed as mechanotransduction and are of fundamental importance to help the cell timely adapt to the continuous dynamic modifications of the microenvironment. Local changes in ECM composition and mechanics are driven by a feed forward interplay between the cell and the matrix itself, with the first depositing ECM proteins that in turn will impact on the surrounding cells. As such, these changes occur regularly during tissue development and are a hallmark of the pathologies of aging. Only lately, though, the importance of mechanical cues in controlling cell function (e.g., proliferation, differentiation, migration) has been acknowledged. Here we provide a critical review of the recent insights into the molecular basis of cellular mechanotransduction, by analyzing how mechanical stimuli get transformed into a given biological response through the activation of a peculiar genetic program. Specifically, by recapitulating the processes involved in the interpretation of ECM remodeling by Focal Adhesions at cell-matrix interphase, we revise the role of cytoskeleton tension as the second messenger of the mechanotransduction process and the action of mechano-responsive shuttling proteins converging on stage and cell-specific transcription factors. Finally, we give few paradigmatic examples highlighting the emerging role of malfunctions in cell mechanosensing apparatus in the onset and progression of pathologies.

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Section: Biological Responses To Cellular Mechanosensingmentioning

confidence: 99%

Cellular Mechanotransduction: From Tension to Function

et al. 2018

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“…Patterning cells via controlling cell confinement within predetermined size, shape, and interconnectivity enables testing of cell morphology dependent fate decision, cytoskeletal tension, cell-cell interaction, etc. (Poudel et al, 2012). Also, when cells are seeded on anisotropic or isotropic topography patterns, cells display contact guided orientation to the anisotropic direction or functional behavior changes depending on the scale of the isotropic topography (Lim and Donahue, 2007).…”

Section: Substrate Surface Patternsmentioning

confidence: 99%

The Role of Microenvironmental Cues and Mechanical Loading Milieus in Breast Cancer Cell Progression and Metastasis

Riehl

Kim

Bouzid

et al. 2021

Front. Bioeng. Biotechnol.

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Cancer can disrupt the microenvironments and mechanical homeostatic actions in multiple scales from large tissue modification to altered cellular signaling pathway in mechanotransduction. In this review, we highlight recent progresses in breast cancer cell mechanobiology focusing on cell-microenvironment interaction and mechanical loading regulation of cells. First, the effects of microenvironmental cues on breast cancer cell progression and metastasis will be reviewed with respect to substrate stiffness, chemical/topographic substrate patterning, and 2D vs. 3D cultures. Then, the role of mechanical loading situations such as tensile stretch, compression, and flow-induced shear will be discussed in relation to breast cancer cell mechanobiology and metastasis prevention. Ultimately, the substrate microenvironment and mechanical signal will work together to control cancer cell progression and metastasis. The discussions on breast cancer cell responsiveness to mechanical signals, from static substrate and dynamic loading, and the mechanotransduction pathways involved will facilitate interdisciplinary knowledge transfer, enabling further insights into prognostic markers, mechanically mediated metastasis pathways for therapeutic targets, and model systems required to advance cancer mechanobiology.

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“…The decreases in adipogenic markers (adiponectin and AP-2) in MSCs by LMS were abolished in the presence of siRNA of SUN. Despite the relatively well-established correlation between MSC adipogenesis vs. osteogenesis in response to mechanical environments [44, 49–51], the LINC complex regulation of MSC osteogenesis by mechanical loading has not been studied intensively. For myogenesis, while the LINC complex has been shown to mediate the mechanical myogenic direction for various myogenic cell lines, there are limited studies on the LINC regulation of the mechanical induction of MSC myogenesis.…”

Section: Linc Complex Regulation Of Msc Function and Fatementioning

confidence: 99%

The LINC complex, mechanotransduction, and mesenchymal stem cell function and fate

et al. 2019

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Mesenchymal stem cells (MSCs) show tremendous promise as a cell source for tissue engineering and regenerative medicine, and are understood to be mechanosensitive to external mechanical environments. In recent years, increasing evidence points to nuclear envelope proteins as a key player in sensing and relaying mechanical signals in MSCs to modulate cellular form, function, and differentiation. Of particular interest is the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex that includes nesprin and SUN. In this review, the way in which cells can sense external mechanical environments through an intact nuclear envelope and LINC complex proteins will be briefly described. Then, we will highlight the current body of literature on the role of the LINC complex in regulating MSC function and fate decision, without and with external mechanical loading conditions. Our review and suggested future perspective may provide a new insight into the understanding of MSC mechanobiology and related functional tissue engineering applications.

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Directing cell function and fate via micropatterning: Role of cell patterning size, shape, and interconnectivity

Cited by 12 publications

References 50 publications

Cellular Mechanotransduction: From Tension to Function

Cellular Mechanotransduction: From Tension to Function

The Role of Microenvironmental Cues and Mechanical Loading Milieus in Breast Cancer Cell Progression and Metastasis

The LINC complex, mechanotransduction, and mesenchymal stem cell function and fate

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