Directing phenotype of vascular smooth muscle cells using electrically stimulated conducting polymer

Rowlands, Andrew; Cooper-White, Justin J.

doi:10.1016/j.biomaterials.2008.07.052

Cited by 76 publications

(45 citation statements)

References 54 publications

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“…All of these results suggest that eGel has adjustable electroactivity, and their conductivity range may be exactly sufficient for in vivo applications due to the microcurrent characteristics. 12,15,35 Fluid uptake capacity of egels…”

Section: Electrochemical Properties and Conductivity Of Hydrogelsmentioning

confidence: 99%

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Wang¹,

Wang²,

Teng³

2016

IJN

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Injectable electroactive hydrogels (eGels) are promising in regenerative medicine and drug delivery, however, it is still a challenge to obtain such hydrogels simultaneously possessing other properties including uniform structure, degradability, robustness, and biocompatibility. An emerging strategy to endow hydrogels with desirable properties is to incorporate functional nanoparticles in their network. Herein, we report the synthesis and characterization of an injectable hydrogel based on oxidized alginate (OA) crosslinking gelatin reinforced by electroactive tetraaniline-graft-OA nanoparticles (nEOAs), where nEOAs are expected to impart electroactivity besides reinforcement without significantly degrading the other properties of hydrogels. Assays of transmission electron microscopy, 1 H nuclear magnetic resonance, and dynamic light scattering reveal that EOA can spontaneously and quickly self-assemble into robust nanoparticles in water, and this nanoparticle structure can be kept at pH 3~9. Measurement of the gel time by rheometer and the stir bar method confirms the formation of the eGels, and their gel time is dependent on the weight content of nEOAs. As expected, adding nEOAs to hydrogels does not cause the phase separation (scanning electron microscopy observation), but it improves mechanical strength up to ~8 kPa and conductivity up to ~10 −6 S/cm in our studied range. Incubating eGels in phosphate-buffered saline leads to their further swelling with an increase of water content <6% and gradual degradation. When growing mesenchymal stem cells on eGels with nEOA content ≤14%, the growth curves and morphology of cells were found to be similar to that on tissue culture plastic; when implanting these eGels on a chick chorioallantoic membrane for 1 week, mild inflammation response appeared without any other structural changes, indicating their good in vitro and in vivo biocompatibility. With injectability, uniformity, degradability, electroactivity, relative robustness, and biocompatibility, these eGels may have a huge potential as scaffolds for tissue regeneration and matrix for stimuli responsive drug release.

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Section: Electrochemical Properties and Conductivity Of Hydrogelsmentioning

confidence: 99%

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Wang¹,

Wang²,

Teng³

2016

IJN

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show abstract

“…[19][20][21] ES has also been successfully applied via PPy for different cell types resulting in increased cell proliferation and significant changes in other cell functions. [22][23][24] This article is the first to report the effects of novel conductive poly-96L/4D-lactide/PPy (PLA-PPy) scaffolds and their combined effects with the ES on hASC viability, proliferation, and early osteogenic differentiation studied in a threedimensional (3D) culture system.…”

mentioning

confidence: 99%

Novel Polypyrrole-Coated Polylactide Scaffolds Enhance Adipose Stem Cell Proliferation and Early Osteogenic Differentiation

Pelto

Björninen

Pälli

et al. 2013

Tissue Engineering Part A

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“…Polypyrrole (PPy) is a commonly used conducting polymer due to its good stability, ease of synthesis, and greater conductivity compared to other conductive polymers [32]. It is used for a wide range of biological applications due to its ability to support growth of electrically responsive cells such as neuronal cells [33], bone cells, and smooth muscle cells [34]. Li et al [32] had synthesized stable, conductive NPs using a copolymer of PPy and 4-sulfonic diphenylamine (SD).…”

Section: Electric and Magnetic Field-responsive Polymersmentioning

confidence: 99%

Development and Characterization of Stimulus-Sensitive Nano/Microparticles for Medical Applications

Menon

Nguyen

2016

Handbook of Nanoparticles

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Directing phenotype of vascular smooth muscle cells using electrically stimulated conducting polymer

Cited by 76 publications

References 54 publications

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Novel Polypyrrole-Coated Polylactide Scaffolds Enhance Adipose Stem Cell Proliferation and Early Osteogenic Differentiation

Development and Characterization of Stimulus-Sensitive Nano/Microparticles for Medical Applications

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