Dirofilaria immitis: Fate and immunogenicity of irradiated infective stage larvae in beagles

Wong, Ming M.; Guest, Mary F.; Lavoipierre, M. M. J.

doi:10.1016/0014-4894(74)90052-6

Cited by 66 publications

(20 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Larvae irradiated with 20 kilorads and subsequently inoculated do not survive beyond 66 days p.i., are not found in the heart or lungs after infection, and do not induce patent infections (145).…”

Section: Dirofilaria Immitismentioning

confidence: 99%

“…4) (143, 144). Autopsy studies 136 days after subcutaneous inoculation demonstrated that 25 to 50% of L3 larvae administered by subcutaneous inoculation develop into adult worms (145). Dogs develop chronic microfilaremia starting at 6 to 7 months p.i.…”

Section: Dirofilaria Immitismentioning

confidence: 99%

“…The best results are obtained when the vaccine is separated from infection by at least 3 months (145). The protective effect appears to be mediated within the first 41 days of challenge infection (145) and is associated with immune responses to specific worm antigens (150,151).…”

Section: Dirofilaria Immitismentioning

confidence: 99%

See 2 more Smart Citations

A Comprehensive, Model-Based Review of Vaccine and Repeat Infection Trials for Filariasis

et al. 2013

View full text Add to dashboard Cite

SUMMARY Filarial worms cause highly morbid diseases such as elephantiasis and river blindness. Since the 1940s, researchers have conducted vaccine trials in 27 different animal models of filariasis. Although no vaccine trial in a permissive model of filariasis has provided sterilizing immunity, great strides have been made toward developing vaccines that could block transmission, decrease pathological sequelae, or decrease susceptibility to infection. In this review, we have organized, to the best of our ability, all published filaria vaccine trials and reviewed them in the context of the animal models used. Additionally, we provide information on the life cycle, disease phenotype, concomitant immunity, and natural immunity during primary and secondary infections for 24 different filaria models.

show abstract

Section: Dirofilaria Immitismentioning

confidence: 99%

Section: Dirofilaria Immitismentioning

confidence: 99%

See 1 more Smart Citation

A Comprehensive, Model-Based Review of Vaccine and Repeat Infection Trials for Filariasis

et al. 2013

View full text Add to dashboard Cite

show abstract

“…This problem has been particularly acute for the study of the infectious form of the parasite, the third stage larva, or L3. Antigens from this stage may be especially important, since it has been shown in other filarial infections that irradiated infective larvae can induce protective immunity (5,6).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of expression cDNA clones encoding antigens of Onchocerca volvulus infective larvae.

Unnasch

Gallin²,

Soboslay³

et al. 1988

J. Clin. Invest.

View full text Add to dashboard Cite

The isolation of recombinant cDNA clones expressing antigens found in Onchocerca volvulus infective larvae is described. To isolate such clones, an expression cDNA library constructed from adult 0. volvulus RNA was screened with antiserum raised against infective larvae. One clone, designated XRAL-1 was characterized further. The recombinant antigen produced by XRAL-1 stimulates proliferation of peripheral blood mononuclear cells from 0. volvulus infected humans. XRAL-1 is derived from a 1450 bases message that encodes a protein with an apparent molecular weight of 42,000 in adult 0. volvulus. The inserted DNA of XRAL-1 contains an open reading frame of 1008 bp. The amino acid sequence predicted by this open reading frame contains three repeats of the sequence KKPEDWD. The identification of clones such as XRAL-1 will provide quantities of purified antigens sufficient to begin to study the immune response to and explore the development of immunity against the infectious form of the parasite.

show abstract

“…These animals were immunized with irradiated L3 because this form of immunization was proven effective elsewhere in several filarial host-parasite systems (3,5,41,46,54). One out of four chimpanzees immunized with irradiated O. volvulus L3 and then infected with untreated L3 demonstrated protective immunity based on the number of microfilariae found in the skin (44).…”

mentioning

confidence: 99%

Development of a Recombinant Antigen Vaccine against Infection with the Filarial WormOnchocerca volvulus

Abraham

Leon

et al. 2001

Infect Immun

View full text Add to dashboard Cite

Efforts to control Onchocerca volvulus, the etiologic agent of river blindness, have been limited to vector control and drug treatment to eliminate microfilariae, with no means available to prevent infection. The goal of this study was to develop a vaccine against this infection using recombinant antigens that are expressed in the early larval stages of the parasite. Five recombinant antigens, Ov7, Ov64, OvB8, Ov9M, and Ov73k, were identified by screening adult and larval cDNA libraries with antibodies from immune humans, chimpanzees, or rabbits. When mice were immunized with the five individual recombinant antigens, statistically significant reductions in parasite survival were induced in mice immunized with Ov7, OvB8, or Ov64, when administered in alum but not when injected in Freund's complete adjuvant (FCA). Live larvae recovered from control and immunized mice were analyzed to determine their developmental stages. A decrease in the percentage of larvae molting from the third stage to the fourth stage was observed with mice immunized with Ov7, Ov64, or OvB8 in alum but not with mice immunized with Ov9 and Ov73k or with mice immunized with any of the five antigens in FCA.

show abstract

Dirofilaria immitis: Fate and immunogenicity of irradiated infective stage larvae in beagles

Cited by 66 publications

References 7 publications

A Comprehensive, Model-Based Review of Vaccine and Repeat Infection Trials for Filariasis

A Comprehensive, Model-Based Review of Vaccine and Repeat Infection Trials for Filariasis

Isolation and characterization of expression cDNA clones encoding antigens of Onchocerca volvulus infective larvae.

Development of a Recombinant Antigen Vaccine against Infection with the Filarial WormOnchocerca volvulus

Contact Info

Product

Resources

About