The occurrence of sexual assault is common and problematic, especially among those with disabilities. While many other characteristics of the victim, perpetrator, and situation involving a sexual assault have been shown to affect perceptions, only one study has examined the impact of a hypothetical victim with a physical disability. Therefore, the purpose of this study was to explore the effect that a victim and/or perpetrator’s physical disability status has on individuals’ classification of encounters as sexual assault. University students over the age of 18 ( n = 207) completed an anonymous online survey which included reading an ambiguous scenario involving a sexual assault in which either the victim, perpetrator or neither was in a wheelchair. Participants classified the scenario as either representing a sexual assault or not and completed the Illinois Rape Myth Acceptance scale and demographic information. A binary logistic regression model was conducted to examine the effects of conditions, participant gender and RMA score on sexual assault classification. In the scenario with the victim in a wheelchair, 71.6% of participants agreed sexual assault occurred; when the perpetrator was in a wheelchair 58.6% classified the scenario as sexual assault. In the control condition 61.4% agreed sexual assault occurred. Condition was not associated with classification at a statistically significant level; however, the effect sizes indicate participants were more likely to classify sexual assault when the victim was in a wheelchair (OR = 1.41), but less likely to blame a perpetrator in a wheelchair (OR = 0.69) compared to the control condition. Despite a lack of statistical significance, the data show a clear trend away from blaming individuals with disabilities in sexual assault scenarios. These findings can have implications within the legal system where incorrect decisions may be made due to bias based on disability status.