Disaccharide Analysis of Skin Glycosaminoglycan in Localized Scleroderma

Abstract

Background: Altered metabolism of skin glycosaminoglycan has rarely been investigated in localized scleroderma in contrast to systemic sclerosis. Objective: The aim of this investigation is to elucidate the change in skin glycosaminoglycan of localized scleroderma. Methods: We analyzed 5 skin samples of localized scleroderma and 10 site-matched control skin samples using high performance liquid chromatography after 1-phenyl-3-methyl-5-pyrasolone labeling. Results: The involved skin constantly showed an increased amount of ΔDi-4S(DS), the main disaccharide unit of dermatan sulfate, a decreased amount of ΔDi-HA, the main disaccharide unit of hyaluronic acid, and an elevated ratio of ΔDi-4S(DS)/ΔDi-HA as compared with the uninvolved skin or the site-matched control skin. Conclusion: These results correlate well with those findings in systemic sclerosis, indicating that the alteration in skin GAG may be closely related to the fibrotic process.

Dermatan sulfate activates nuclear factor-κb and induces endothelial and circulating intercellular adhesion molecule-1

“…We demonstrate here that the soluble form of this material stimulates expression of ICAM-1. ICAM-1 has been found to be abundantly expressed in cutaneous disease such as scleroderma (18)(19)(20), which is also characterized by an increase in DS (35)(36)(37). It is attractive to speculate that DS released from the dermis may contribute to such disease processes by inducing ICAM-1.…”

Localized scleroderma/morphea

“…3), complemented by an inflammatory infiltrate in the mid‐dermis. Yokoyama et al 47 . studied disaccharide analysis of skin glycosaminoglycan in localized scleroderma, and demonstrated its altered pattern.…”

Disaccharide analysis of skin glycosaminoglycan in lichen myxedematosus

“…1,2 The procedures for RNA extraction and polymerase chain reactions (PCR) are described before. 1,2 The procedures for RNA extraction and polymerase chain reactions (PCR) are described before.…”

“…We report a 74-yearold male patient who had an immediate reaction and suffered angioedema and dyspnoea after having eaten approx. 2,3,5 Specific IgE diagnostics are not reliable. Following emergency medical attention, the patient had to be monitored in hospital.…”