Disappearance of 2,4-Dinitrotoluene and 2-Amino,4,6-Dinitrotoluene by Phanerochaete chrysosporium Under Non-Ligninolytic Conditions

Jackson, Marcus; Hou, Li; Banerjee, Hirendra Nath; Sridhar, R.; Dutta, S. K.

doi:10.1007/s001289900887

Cited by 17 publications

(10 citation statements)

References 14 publications

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“…The importance of the white-rot fungus (Phanerochaete chrysosporium strain BKM-F-1767) lies in the presence of an extra-cellular ligninolytic system which is involved in the degradation of lignin and a wide variety of halogenated and non-halogenated organic compounds to CO 2 [13] under low nitrogen (ligninolytic) conditions. New findings in our laboratory indicate that this fungus can also degrade toxic chemicals under nitrogen-rich (nonligninolytic) conditions [14][15][16]. It was apparent that there must be another mechanism of PCB degradation by this fungus.…”

Section: Introductionmentioning

confidence: 96%

Nitrate reductase gene involvement in hexachlorobiphenyl dechlorination by Phanerochaete chrysosporium

Perkins

Dutta

2006

Journal of Hazardous Materials

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Section: Introductionmentioning

confidence: 96%

Nitrate reductase gene involvement in hexachlorobiphenyl dechlorination by Phanerochaete chrysosporium

Perkins

Dutta

2006

Journal of Hazardous Materials

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“…94% the 2,4-DNT concentration, whereas there was evidently a 58% reduction produced by the parent strain USDA 1936. A previous report also showed the use of HPLC (Jackson et al, 1999) to determine the ability of soil microorganisms to degrade nitroaromatic compounds.…”

Section: Discussionmentioning

confidence: 99%

“…The samples were then spun at 5049g for 5 min. The test procedure for nitrite release is essentially as described by Jackson et al (1999). Supernatant samples were assayed for the presence of nitrite by combining 0.5 ml of each supernatant to 0.5 ml of 1% (w/v) sulfanilamide in 1.5N HCl and 0.5 ml of 0.02% (w/v) N-1-naphthyl ethylenediamine (Daniels et al, 1994).…”

Section: Nitrite Assaymentioning

confidence: 99%

Enhanced bioremediation of soil containing 2,4-dinitrotoluene by a genetically modified Sinorhizobium meliloti

Dutta

Hollowell

Hashem

et al. 2003

Soil Biology and Biochemistry

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Hollowell, Gail P.; Hashem, Fawzy M.; and Kuykendall, L. David, "Enhanced bioremediation of soil containing 2,4-dinitrotoluene by a genetically modified Sinorhizobium meliloti" (2003 AbstractThe symbiotic nitrogen-fixing soil bacterium, Sinorhizobium meliloti, is well known for its ability to interact with the leguminous plant Medicago sativa L. It has, however, not been reported that this species possesses the capability to degrade toxic nitroaromatic compounds, such as 2,4-dinitrotoluene (DNT) which is commonly associated with the degradation of the explosive trinitrotoluene (TNT). In this study, the pJS1 DNT-biodegradative plasmid was genetically transferred to S. meliloti strain USDA 1936, which was confirmed by plasmid profile analysis. Several standard analytical and chemical tests including high performance liquid chromatography (HPLC), nitrite (NO 2 ) release assays, rhizosphere population and plant greenhouse studies were conducted to test the ability of S. meliloti to degrade 2,4-DNT. The possible presence of 2,4-DNT remaining in the treated soil was tested, and no 2,4-DNT had been absorbed by the soil. The pJS1-carrying recombinant strain DHK1 produced 'ARC' alfalfa plants that were almost 2-fold higher in shoot dry weight than that produced by the parent strain on soil containing 0.14 mM 2,4-DNT. The transconjugant strain DHK1 reduced significantly one-third more 2,4-DNT in both 0.14 and 0.28 mM contaminated soil, and in 0.55 mM contaminated soil it degraded 94% of the 2,4-DNT present. In liquid cultures, however, only about 4% reduction in 2,4-DNT concentrations was obtained in 10 days. We interpret the results as clearly establishing that genetic modification was successfully used, for the first time, to improve the capability of the symbiotic nitrogen-fixing soil bacterium S. meliloti DHK1 to bioremediate in situ 2,4-DNT-contaminated soil in the presence of alfalfa plants. q

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“…Donnelly et al 1997 demonstrated that several white-rot fungi are able to transform TNT to DNTs. Jackson et al (1999) reported degradation of TNT by non-ligninolytic strains of P. chrysosporium. Significantly, only white-rot fungi have been shown as capable of DNT degradation and mineralization to CO 2 (Hodgson et al 2000).…”

Section: Tntmentioning

confidence: 99%

White-Rot Fungi in Bioremediation

2012

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Disappearance of 2,4-Dinitrotoluene and 2-Amino,4,6-Dinitrotoluene by Phanerochaete chrysosporium Under Non-Ligninolytic Conditions

Cited by 17 publications

References 14 publications

Nitrate reductase gene involvement in hexachlorobiphenyl dechlorination by Phanerochaete chrysosporium

Nitrate reductase gene involvement in hexachlorobiphenyl dechlorination by Phanerochaete chrysosporium

Enhanced bioremediation of soil containing 2,4-dinitrotoluene by a genetically modified Sinorhizobium meliloti

White-Rot Fungi in Bioremediation

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