Disappearance of the Surface Sheath from Dictyostelium discoideum Sorus during Late Culmination.

The Formation of Actin Rods Composed of Actin Tubules in Dictyostelium discoideum Spores

“…To examine the relative orientation of actin filaments on a wall of an actin tubule to that of neighboring tubules by image averaging, the image of each tubule was rotated and overlaid once to four times at an angle of 180°, 120°, 90°, or 72°, respectively, using Adobe Photoshop (Adobe Systems Inc., San Jose, CA). For scanning electron microscopy, developing cells on dialysis membranes were exposed to acrolein vapor (Sameshima, 1985(Sameshima, , 1993. After 5 min of exposure, membranes were immersed in PB containing 4% paraformaldehyde (pH 6.8) for 1 h and then fixed with 1.5% OsO 4 for 30 min on ice.…”

Novel Actin Rods Appeared in Spores of Dictyostelium discoideum.

“…Fruiting bodies were formed after 3 days. For synchronous development, growing cells were freed from bacteria with low speed centrifugation, suspended in Bonner's salt solution (BSS) containing ll mMKC1, ll mMNaCl, and 3 mMCaCl2, and then spread on non-nutrient agar plates (13). After 24 h fruiting bodies appeared.…”

S‐Adenosyl‐L‐homocysteine hydrolase is sequestered into actin rods in Dictyostelium discoideum spores

“…The slime mold, Dictyostelium discoideum , is a primitive eukaryote that shows a well‐defined developmental process upon depletion of food sources [8]. The amoebae migrate toward cAMP, which acts as a chemoattractant, and undergo multicellular development to form a fruiting body carrying a mass of spores into the air; the suspended mass of spores is known as the sorus [9]. The spores have large bundles of actin filaments called actin rods in both the nucleus and cytoplasm (Fig.…”