Three groups of composite material layers are used in this work to measure the mechanical properties of ankle disarticulation prosthetic socket. The polymer matrix acrylic (Lamination 80:20) and reinforced with different materials, the first type is (4Perlon) layers, second type (2 Perlon and 4 Glass fiber and 2 Perlon) layers and third type is including (2 Perlon, 4 Carbon fiber and 2 Perlon) layers used in this study. Tests Results showed that the mechanical properties of all groups, ultimate strength, yield stress and Young modulus for the first group; were 10.12MPa, 38.8MPa and 1.16GPa respectively, for second group were73.8MPa, 146MPa and 1.45GPa respectively and for the third group were 106.6MPa, 209.4MPa and 1.9GPa respectively. The results of the bacteria test growth show inability of bacteria (Staphylococcus aureus) to analyze of the surfaces of materials available and suggested for feeding it therefore bacteria can grow on them. By using (F-socket) the pressure between stump and socket measured and the values are (236kPa) and (275kPa) for the lateral and posterior reign. From fatigue test for all groups and ANSYS 14.5 software, the safety factors for composite material (2Perlon, 4carbon fiber,2perlon) layers is about (1.57) which are safe and acceptable in design applications.