Discordant expression and variable numbers of neighboring GGA- and GAA-rich triplet repeats in the 3' untranslated regions of two groups of messenger RNAs encoded by the rat polymeric immunoglobulin receptor gene

Abstract

ABSTRACT

Characterization of the Human Polymeric Immunoglobulin Receptor <i>(PIGR)</i> 3&prime;UTR and Differential Expression of <i>PIGR</i> mRNA during Colon Tumorigenesis

“…Following the AluSx element is a direct repeat of the terminal 13-bp of the MIR element (not found at the orthologous position of the bovine PIGR gene), which may have amplified prior to or as a consequence of insertion of the Alu elements. Interestingly, the 3)UTR of the rat PIGR gene contains variable numbers of neighboring GGA-and GAA-rich triplet repeats at a position orthologous to the Alu elements in the human PIGR 3)UTR [20]. The presence of multiple contiguous repetitive elements in the PIGR 3)UTR suggests that this region may have represented a 'hotspot' for DNA recombination during mammalian evolution.…”

“…Second, none of the reported cDNA sequences contained a consensus polyadenylation signal in the predicted 3)UTR. Third, the complete 3)UTRs for bovine, rat, mouse and rabbit PIGR mRNAs had been shown to range in length from 1,072 to 1,434 nt [20,26,27,32]. Despite considerable effort on the part of several groups, technical problems prevented the isolation of full-length cDNA clones for human PIGR by screening of cDNA libraries.…”

Intramolecular Higher Order Packing of Parallel Quadruplexes Comprising a G:G:G:G Tetrad and a G(:A):G(:A):G(:A):G Heptad of GGA Triplet Repeat DNA

“…The GGA repeat has been identified in portions of human and mouse cellular DNA that cross-hybridize with the internal direct repeat (IR3) repetitive region of Epstein-Barr virus (8). The GGA repeat, together with the GAA repeat, has also been found in micro satellite DNA belonging to the rat polymeric immunoglobulin receptor gene (9). A fragment of the microsatellite DNA containing both the repeats was suggested to attenuate gene expression at the transcriptional and post-transcriptional levels (10).…”

Hepatic Progenitor Cell Lines from Allyl Alcohol‐Treated Adult Rats Are Derived from γ‐Irradiated Mouse STO Cells

“…The combined observations indicate that STO and LPC cells were & and necessitated identification of species of origin. Therefore, DNA from authentic rat liver, STO cells, and several LPCs (3(8)#21-EGFP P22 and P56, 3(8)#21 P22, and PC4 [subcloned 3(8)#21-EGFP P28]) were analyzed for 3′ untranslated region triplet repeat microsatellite polymorphisms that were encoded by rat [19] and mouse Pigr genes [20] located on chromosomes 13 and 1, respectively [15]. Except for rat liver, which generated an expected ~320-to 330-bp product, all other samples generated larger products of ~350-to 360-bp.…”