Discovery and analysis of Bartonella henselae antigens for use in clinical serologic assays

McCool, Tera L.; Hoey, John; Montileone, Francesca; Goldenberg, Hannah; Mordechai, Eli; Adelson, Martin E.

doi:10.1016/j.diagmicrobio.2007.07.017

Cited by 15 publications

(29 citation statements)

References 14 publications

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“…Both spots are reported (McCool et al , 2008) to be good markers of CSD. However, our results have not completely confirmed their results: firstly, we found a lower rate of CSD patients' sera reactivity to SodB [sensitivity (Se) 28.5%] compared with that reported by McCool et al (2008) (71%) and sera from IE patients (Se 86%), and secondly, a huge rate of cross‐reactivity to GroEL among BD was found [specificity (Sp) 25%] in contrast to that obtained by McCool et al (2008), and GroEL was highly specific (100%) (Table 2). This result is not surprising considering that GroEL is a very well‐conserved protein.…”

Section: Resultsmentioning

confidence: 99%

Identification of candidate proteins for the diagnosis of Bartonella henselae infections using an immunoproteomic approach

Saisongkorh

Kowalczewska

Azza

et al. 2010

FEMS Microbiology Letters

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Bartonella henselae is an emerging gram-negative facultative intracellular pathogen transmitted via Ctenocephalides felis (cat fleas) or cat scratches. Bartonellosis is present mainly in the form of cat scratch disease (CSD), bacillary angiomatosis and infective endocarditis (IE). The methods used to diagnose B. henselae rely on culturing, immunofluorescent assays and molecular techniques. The objective of the present study was to identify candidate proteins for the serodiagnosis of bartonellosis with the differential discrimination of both clinical scenarios: CSD and IE. For this, an immunoproteomic approach combined with 2-DE, immunoblotting and matrix-assisted laser desorption/ionization time-of-flight MS has been developed. Immunoproteomic profiles of sera collected from patients with CSD and IE were compared with those of blood donors. We identified several candidate proteins as phage-encoding Pap31 protein and an outer membrane protein of BH11510 that, in our view, might be useful for the serodiagnosis of bartonellosis.

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Section: Resultsmentioning

confidence: 99%

Identification of candidate proteins for the diagnosis of Bartonella henselae infections using an immunoproteomic approach

Saisongkorh

Kowalczewska

Azza

et al. 2010

FEMS Microbiology Letters

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“…Moreover, the results of IFA testing may be influenced by antigenic variance as some strains are deficient in their expression of the immunodominant Bartonella adheson A (BadA) (Riess et al, 2007) or by interobserver variability. Further attempts to improve the serological algorithms (e.g., by defining immunodominant proteins) are highly desirable and have already led to the identification of several seroreactive potential marker proteins (Eberhardt et al, 2009;Wagner et al, 2008;McCool et al, 2008;Boonjakuakul et al, 2007). Bartonella spp.…”

Section: Bartonella Infections: Diagnostic Proceduresmentioning

confidence: 99%

Bartonella spp.: Throwing light on uncommon human infections

Kaiser

Rieß

O’Rourke

et al. 2011

International Journal of Medical Microbiology

106

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“…The same approach has also been used to identify immunoreactive proteins of Bartonella quintana and Bartonella henselae [21], [22], [23]. In this study, we combined an immunoproteomic method, i.e.…”

Section: Introductionmentioning

confidence: 99%

Identification of Immunogenic Proteins of Waddlia chondrophila

et al. 2012

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Evidence is growing for a role of Waddlia chondrophila as an agent of adverse pregnancy outcomes in both humans and ruminants. This emerging pathogen, member of the order Chlamydiales, is also implicated in bronchiolitis and lower respiratory tract infections. Until now, the serological diagnosis of W. chondrophila infection has mainly relied on manually intensive tests including micro-immunofluorescence and Western blotting. Thus, there is an urgent need to establish reliable high throughput serological assays. Using a combined genomic and proteomic approach, we detected 57 immunogenic proteins of W. chondrophila, of which 17 were analysed by mass spectrometry. Two novel hypothetical proteins, Wim3 and Wim4, were expressed as recombinant proteins in Escherichia coli, purified and used as antigens in an ELISA test. Both proteins were recognized by sera of rabbits immunized with W. chondrophila as well as by human W. chondrophila positive sera but not by rabbit pre-immune sera nor human W. chondrophila negative sera. These results demonstrated that the approach chosen is suitable to identify immunogenic proteins that can be used to develop a serological test. This latter will be a valuable tool to further clarify the pathogenic potential of W. chondrophila.

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Discovery and analysis of Bartonella henselae antigens for use in clinical serologic assays

Cited by 15 publications

References 14 publications

Identification of candidate proteins for the diagnosis of Bartonella henselae infections using an immunoproteomic approach

Identification of candidate proteins for the diagnosis of Bartonella henselae infections using an immunoproteomic approach

Bartonella spp.: Throwing light on uncommon human infections

Identification of Immunogenic Proteins of Waddlia chondrophila

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