Discovery and Biosynthesis of the Amodesmycins, Aromatic Polyketide-Siderophore Hybrid Conjugates

Abstract

A type II polyketide synthase biosynthetic gene cluster (amd) containing three P450 genes was identified from a soil metagenomic library, and novel benz[h]isoquinoline-desferrioxamine B conjugated compound amodesmycins were isolated from Streptomyces albus J1074 harboring the amd gene cluster. Genetic evidence showed that the benz[h]isoquinoline part and desferrioxamine B part in amodesmycins were derived from the amd gene cluster and S. albus J1074, respectively, while P450 enzymes played critical roles in the conjunction of these two parts.

Siderophore conjugates to combat antibiotic-resistant bacteria

“…In addition to this, a new family of sideromycins called the amodesmycins were recently isolated and identified from Streptomyces albus J1074, however, these compounds displayed no significant activity against the test panel of bacteria so will not be discussed any further. 40…”

“…In addition to this, a new family of sideromycins called the amodesmycins were recently isolated and identified from Streptomyces albus J1074, however, these compounds displayed no significant activity against the test panel of bacteria so will not be discussed any further. 40 Albomycin (1) (Fig. 3) (originally known as grisein) is a siderophore-antibiotic conjugate that was first isolated from Streptomyces griseus in the 1940s and is the most highly studied of the sideromycins.…”