Discovery and characterization of a novel splice variant of the GM-CSF receptor α subunit

Pelley, Jennifer L.; Nicholls, Chris D.; Beattie, Tara L.; Brown, Christopher B.

doi:10.1016/j.exphem.2007.06.008

Cited by 9 publications

(4 citation statements)

References 38 publications

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“…Alternative splicing is one of the modifications reported in several genes (Blencowe, 2006). Human neutrophils have been reported to have several alternative splice variants of functional genes such as Fcα receptor (Pleass et al, 1996), caspase-8 (Eckhart et al, 2001), glucocorticoid receptor (Strickland et al, 2001) phospholipase D (Di Fulvio andGomez-Cambronero, 2005), glutaredoxin 1 (Park and Levine, 2005) and GM-CSF receptor α (Pelley et al, 2007). In the present study, we demonstrated that pantetheinase genes expressed in human leukocytes are also subject to such a modification, and that there are several mRNA variants of human GPI-80/VNN2 and VNN3.…”

Section: Discussionmentioning

confidence: 97%

Alternative spliced variants in the pantetheinase family of genes expressed in human neutrophils

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Inoue

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2008

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Section: Discussionmentioning

confidence: 97%

Alternative spliced variants in the pantetheinase family of genes expressed in human neutrophils

Nitto

Inoue

Node

2008

Gene

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“…OCSTAMP promotes the fusion of cells formed by osteoclasts or foreign body giant cells and participates in osteoclast-mediated bone absorption, thus playing an important role in the differentiation and functional maturation of multinucleated osteoclasts ( 39 ). CSF2R and CSF3R, receptors of CSF2 and CSF3, respectively, are considered crucial in the generation, proliferation, differentiation, and functioning of the granulocytes and macrophages ( 40 , 41 ). In arthritis, MMPs and cathepsin secreted from the osteoclast, macrophage, or fibroblast take part in destruction of the joints by degrading the cartilage and extracellular matrix ( 42 ).…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profiles Analyzed Using Integrating RNA Sequencing, and Microarray Reveals Increased Inflammatory Response, Proliferation, and Osteoclastogenesis in Pigmented Villonodular Synovitis

Zhao

Zhang

et al. 2021

Front. Immunol.

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BackgroundPigmented villonodular synovitis (PVNS) is a rare condition that involves benign proliferation of the synovial tissue and is characterized by severe joint destruction and high recurrence even after surgical resection. However, poor understanding of the pathogenesis limits its effective therapy.MethodIn this study, gene expression profiles of six patients with PVNS, 11 patients with osteoarthritis (OA), nine patients with rheumatoid arthritis (RA) (E-MTAB-6141), and three healthy subjects (GSE143514) were analyzed using integrating RNA sequencing (RNA-seq) and microarray to investigate the PVNS transcriptome. Gene ontology, string, and cytoscape were used to determine the gene functional enrichment. Cell functional molecules were detected using flow cytometry or immunohistochemical test to identify the cell subset and function. CD14+ cells were isolated and induced to osteoclast to evaluate the monocyte/macrophage function.ResultsThe most obvious local manifestations of PVNS were inflammation, including increased immune cells infiltration and cytokine secretion, and tumor phenotypes. High proportion of inflammatory cells, including T cells, natural killer (NK) cells, NKT cells, and B cells were recruited from the blood. Th17 and monocytes, especially classical monocytes but not nonclassical monocytes, increased in PVNS synovium. An obvious increase in osteoclastogenesis and macrophage activation was observed locally. Elevated expression of MMP9, SIGLEC 15, and RANK were observed in myeloid cell of PVNS than OA. When compared with RA, osteoclast differentiation and myeloid cell activation are PVNS-specific characters, whereas T cell activation is shared by PVNS and RA.ConclusionThe transcriptional expression characteristics of PVNS showed increased immune response, cell migration, and osteoclastogenesis. Osteoclast differentiation is only observed in PVNS but not RA, whereas T-cell activation is common in inflammatory arthritis.

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“…and CSF3R, receptors of CSF2 and CSF3, respectively, are considered crucial in the generation, proliferation, differentiation, and functioning of the granulocytes and macrophages (40,41). In arthritis, MMPs and cathepsin secreted from the osteoclast, macrophage, or broblast take part in destruction of the joints by degrading the cartilage and extracellular matrix (42).…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profiles Analyzed Using Integrating RNA Sequencing and Microarray Reveals Increased Inflammatory Response, Proliferation, and Osteoclastogenesis in Pigmented Villonodular Synovitis

Zhao

Zhang

et al. 2021

Preprint

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Background: Pigmented villonodular synovitis (PVNS) is a rare condition that involves benign proliferation of the synovial tissue and is characterized by severe joint destruction and high recurrence even after surgical resection. However, poor understanding of the pathogenesis limits its effective therapy.Method: In this study, gene expression profiles of six patients with PVNS and six patients with osteoarthritis (OA) were analyzed using integrating RNA sequencing (RNA-seq) and microarray to investigate the PVNS transcriptome. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, string, and cytoscape were used to determine the gene functional enrichment. Cell surface functional molecules were detected using flow cytometry to identify the cell subset. CD14 positive cells were isolated and induced to differentiate into osteoclast to evaluate the monocyte/macrophage function.Results: RNA-seq and microarray data revealed 195 common differentially expressed genes; expression change trends were consistent in both the methods. The most obvious local manifestations of PVNS were inflammation and tumor phenotypes. Infiltration of a large number of immune cells and increased cytokine secretion induced inflammation. High proportion of CD45+ inflammatory cells, including T cells (CD3+), natural killer cells (CD3-CD56+), NKT cells (CD3+CD56+), and B cells (CD19+), were recruited from the blood. Cell proliferation and migration resulted in manifestation of the tumor phenotype. Tumor checkpoint molecules, such as programmed cell death protein 1, T cell immunoglobulin and mucin domain 3, lymphocyte-activation gene 3, cytotoxic T lymphocyte-associated protein 4, and sialic-acid-binding immunoglobulin-like lectin, were expressed on the surface of CD4+ and CD8+ T cells. In addition, an obvious increase in osteoclastogenesis and macrophage activation were observed locally. Tartrate-resistant acid phosphatase-positive mature osteoclasts that differentiated from CD14+ monocytes were significantly higher in the PVNS synovium than that in OA. Conclusion: The transcriptional expression characteristics of PVNS resulted in increased immune response and cytokine expression, high cell proliferation and migration, and increased osteoclastogenesis and bone injury.

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Discovery and characterization of a novel splice variant of the GM-CSF receptor α subunit

Cited by 9 publications

References 38 publications

Alternative spliced variants in the pantetheinase family of genes expressed in human neutrophils

Alternative spliced variants in the pantetheinase family of genes expressed in human neutrophils

Gene Expression Profiles Analyzed Using Integrating RNA Sequencing, and Microarray Reveals Increased Inflammatory Response, Proliferation, and Osteoclastogenesis in Pigmented Villonodular Synovitis

Gene Expression Profiles Analyzed Using Integrating RNA Sequencing and Microarray Reveals Increased Inflammatory Response, Proliferation, and Osteoclastogenesis in Pigmented Villonodular Synovitis

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